Donna Francy (Former Employee)
Science and Products
Filter Total Items: 46
Use of Spatial Sampling and Microbial Source-Tracking Tools for Understanding Fecal Contamination at Two Lake Erie Beaches
Source-tracking tools were used to identify potential sources of fecal contamination at two Lake Erie bathing beaches: an urban beach (Edgewater in Cleveland, Ohio) and a beach in a small city (Lakeshore in Ashtabula, Ohio). These tools included identifying spatial patterns of Escherichia coli (E. coli) concentrations in each area, determining weather patterns that caused elevated E. coli, and app
Authors
Donna S. Francy, Erin E. Bertke, Dennis P. Finnegan, Christopher M. Kephart, Rodney A. Sheets, John Rhoades, Lester Stumpe
A spatial, multivariable approach for identifying proximate sources of Escherichia coli to Maumee Bay, Lake Erie, Ohio
No abstract available.
Authors
Donna S. Francy, Pamela Struffolino, Amie M. G. Brady, Daryl F. Dwyer
Effects of seeding procedures and water quality on recovery of Cryptosporidium oocysts from stream water by using U.S. Environmental Protection Agency method 1623
U.S. Environmental Protection Agency method 1623 is widely used to monitor source waters and drinking water supplies for Cryptosporidium oocysts. Matrix spikes, used to determine the effect of the environmental matrix on the method's recovery efficiency for the target organism, require the collection and analysis of two environmental samples, one for analysis of endemic oocysts and the other for a
Authors
D.S. Francy, O. D. Simmons, M.W. Ware, E.J. Granger, M.D. Sobsey, F. W. Schaefer
Environmental factors and chemical and microbiological water-quality constituents related to the presence of enteric viruses in ground water from small public water supplies in southeastern Michigan
A study of small public ground-water-supply wells that produce water from discontinuous sand and gravel aquifers was done from July 1999 through July 2001 in southeastern Michigan. Samples were collected to determine the occurrence of viral pathogens and microbiological indicators of fecal contamination (“indicators”), determine whether indicators are adequate predictors of the presence of enteric
Authors
Donna S. Francy, Rebecca N. Bushon, Julie Stopar, Emma J. Luzano, G. Shay Fout
Detection of human enteric viruses in stream water with RT-PCR and cell culture
A multiplex RT-PCR method was used to measure virus occurrence at five stream water sites that span a range of hydroclimatic, water-quality, and land-use characteristics. The performance of the molecular method was evaluated in comparison with traditional cell culture and Escherichia coli membrane filtration assays. The study incorporated multiple quality controls and included a control for virus
Authors
K. Denis-Mize, G.S. Fout, D.R. Dahling, D.S. Francy
Escherichia coli at Ohio bathing beaches—Distribution, sources, wastewater indicators, and predictive modeling
Results of studies during the recreational seasons of 2000 and 2001 strengthen the science that supports monitoring of our Nation's beaches. Water and sediment samples were collected and analyzed for concentrations of Escherichia coli (E. coli). Ancillary water-quality and environmental data were collected or compiled to determine their relation to E. coli concentrations. Data were collected at t
Authors
Donna S. Francy, Amie M. Gifford, Robert A. Darner
Chapter A7. Section 7.3. Protozoan Pathogens
Protozoan pathogens are widely distributed in the aquatic environment. Cryptosporidium and Giardia are the principal protozoan pathogens that are known to affect the acceptability of water supplies for public use within the United States. A sampling program for protozoan pathogens should be conducted over an extended period of time because of cyclical and seasonal variations in their concentration
Authors
Rebecca N. Bushon, Donna S. Francy
Escherichia coli in the swash zone at four Ohio bathing beaches
The zone of the shoreline that is constantly washed by waves or tides, called the swash zone, is an attractive recreational area, especially for children who play in the sand. The swash zone, however, has been suggested as a possible habitat for waterborne disease-causing microorganisms (pathogens). The spaces between the sand grains, or interstices, offer habitats that may support the survival of
Authors
Donna S. Francy, Amie M. Gifford
Forecasting bacteria levels at bathing beaches in Ohio
The U.S. Geological Survey developed models for predicting exceedance of the bathing-water standard for Escherichia coli (E. coli) at three Lake Erie beaches and one inland lake in Ohio. The statistical models were specific to each beach, and the best model for each beach was based on a unique combination of environmental and water-quality variables as explanatory factors. For the Lake Erie beaches
Authors
Donna S. Francy, Robert A. Darner
Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters
To improve surveillance for Cryptosporidium oocysts in water, the US Environmental Protection Agency developed method 1622, which consists of filtration, concentration, immunomagnetic separation, fluorescent antibody and 4, 6-diamidino-2-phenylindole (DAPI) counter-staining, and microscopic evaluation. Two filters were compared for analysis of 11 stream water samples collected throughout the Unite
Authors
O. D. Simmons, M.D. Sobsey, F. W. Schaefer, D.S. Francy, Rebecca A. Nally, C.D. Heaney
Concentration and Detection of Cryptosporidium Oocysts in Surface Water Samples by Method 1622 Using Ultrafiltration and Capsule Filtration
The protozoan parasite Cryptosporidium parvum is known to occur widely in both source and drinking water and has caused waterborne outbreaks of gastroenteritis. To improve monitoring, the U.S. Environmental Protection Agency developed method 1622 for isolation and detection of Cryptosporidium oocysts in water. Method 1622 is performance based and involves filtration, concentration, immunomagnetic
Authors
O. D. Simmons, M.D. Sobsey, C.D. Heaney, F. W. Schaefer, D.S. Francy
Microbiological monitoring for the U.S. Geological Survey National Water-Quality Assessment Program
Data to characterize the microbiological quality of the Nation's fresh, marine, and estuarine waters are usually collected for local purposes, most often to judge compliance with standards for protection of public health in swimmable or drinkable waters. Methods and procedures vary with the objectives and practices of the parties collecting data and are continuously being developed or modified. Th
Authors
Donna S. Francy, Donna N. Myers, Dennis R. Helsel
Science and Products
Filter Total Items: 46
Use of Spatial Sampling and Microbial Source-Tracking Tools for Understanding Fecal Contamination at Two Lake Erie Beaches
Source-tracking tools were used to identify potential sources of fecal contamination at two Lake Erie bathing beaches: an urban beach (Edgewater in Cleveland, Ohio) and a beach in a small city (Lakeshore in Ashtabula, Ohio). These tools included identifying spatial patterns of Escherichia coli (E. coli) concentrations in each area, determining weather patterns that caused elevated E. coli, and app
Authors
Donna S. Francy, Erin E. Bertke, Dennis P. Finnegan, Christopher M. Kephart, Rodney A. Sheets, John Rhoades, Lester Stumpe
A spatial, multivariable approach for identifying proximate sources of Escherichia coli to Maumee Bay, Lake Erie, Ohio
No abstract available.
Authors
Donna S. Francy, Pamela Struffolino, Amie M. G. Brady, Daryl F. Dwyer
Effects of seeding procedures and water quality on recovery of Cryptosporidium oocysts from stream water by using U.S. Environmental Protection Agency method 1623
U.S. Environmental Protection Agency method 1623 is widely used to monitor source waters and drinking water supplies for Cryptosporidium oocysts. Matrix spikes, used to determine the effect of the environmental matrix on the method's recovery efficiency for the target organism, require the collection and analysis of two environmental samples, one for analysis of endemic oocysts and the other for a
Authors
D.S. Francy, O. D. Simmons, M.W. Ware, E.J. Granger, M.D. Sobsey, F. W. Schaefer
Environmental factors and chemical and microbiological water-quality constituents related to the presence of enteric viruses in ground water from small public water supplies in southeastern Michigan
A study of small public ground-water-supply wells that produce water from discontinuous sand and gravel aquifers was done from July 1999 through July 2001 in southeastern Michigan. Samples were collected to determine the occurrence of viral pathogens and microbiological indicators of fecal contamination (“indicators”), determine whether indicators are adequate predictors of the presence of enteric
Authors
Donna S. Francy, Rebecca N. Bushon, Julie Stopar, Emma J. Luzano, G. Shay Fout
Detection of human enteric viruses in stream water with RT-PCR and cell culture
A multiplex RT-PCR method was used to measure virus occurrence at five stream water sites that span a range of hydroclimatic, water-quality, and land-use characteristics. The performance of the molecular method was evaluated in comparison with traditional cell culture and Escherichia coli membrane filtration assays. The study incorporated multiple quality controls and included a control for virus
Authors
K. Denis-Mize, G.S. Fout, D.R. Dahling, D.S. Francy
Escherichia coli at Ohio bathing beaches—Distribution, sources, wastewater indicators, and predictive modeling
Results of studies during the recreational seasons of 2000 and 2001 strengthen the science that supports monitoring of our Nation's beaches. Water and sediment samples were collected and analyzed for concentrations of Escherichia coli (E. coli). Ancillary water-quality and environmental data were collected or compiled to determine their relation to E. coli concentrations. Data were collected at t
Authors
Donna S. Francy, Amie M. Gifford, Robert A. Darner
Chapter A7. Section 7.3. Protozoan Pathogens
Protozoan pathogens are widely distributed in the aquatic environment. Cryptosporidium and Giardia are the principal protozoan pathogens that are known to affect the acceptability of water supplies for public use within the United States. A sampling program for protozoan pathogens should be conducted over an extended period of time because of cyclical and seasonal variations in their concentration
Authors
Rebecca N. Bushon, Donna S. Francy
Escherichia coli in the swash zone at four Ohio bathing beaches
The zone of the shoreline that is constantly washed by waves or tides, called the swash zone, is an attractive recreational area, especially for children who play in the sand. The swash zone, however, has been suggested as a possible habitat for waterborne disease-causing microorganisms (pathogens). The spaces between the sand grains, or interstices, offer habitats that may support the survival of
Authors
Donna S. Francy, Amie M. Gifford
Forecasting bacteria levels at bathing beaches in Ohio
The U.S. Geological Survey developed models for predicting exceedance of the bathing-water standard for Escherichia coli (E. coli) at three Lake Erie beaches and one inland lake in Ohio. The statistical models were specific to each beach, and the best model for each beach was based on a unique combination of environmental and water-quality variables as explanatory factors. For the Lake Erie beaches
Authors
Donna S. Francy, Robert A. Darner
Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters
To improve surveillance for Cryptosporidium oocysts in water, the US Environmental Protection Agency developed method 1622, which consists of filtration, concentration, immunomagnetic separation, fluorescent antibody and 4, 6-diamidino-2-phenylindole (DAPI) counter-staining, and microscopic evaluation. Two filters were compared for analysis of 11 stream water samples collected throughout the Unite
Authors
O. D. Simmons, M.D. Sobsey, F. W. Schaefer, D.S. Francy, Rebecca A. Nally, C.D. Heaney
Concentration and Detection of Cryptosporidium Oocysts in Surface Water Samples by Method 1622 Using Ultrafiltration and Capsule Filtration
The protozoan parasite Cryptosporidium parvum is known to occur widely in both source and drinking water and has caused waterborne outbreaks of gastroenteritis. To improve monitoring, the U.S. Environmental Protection Agency developed method 1622 for isolation and detection of Cryptosporidium oocysts in water. Method 1622 is performance based and involves filtration, concentration, immunomagnetic
Authors
O. D. Simmons, M.D. Sobsey, C.D. Heaney, F. W. Schaefer, D.S. Francy
Microbiological monitoring for the U.S. Geological Survey National Water-Quality Assessment Program
Data to characterize the microbiological quality of the Nation's fresh, marine, and estuarine waters are usually collected for local purposes, most often to judge compliance with standards for protection of public health in swimmable or drinkable waters. Methods and procedures vary with the objectives and practices of the parties collecting data and are continuously being developed or modified. Th
Authors
Donna S. Francy, Donna N. Myers, Dennis R. Helsel