Robert Bonde, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 93
Monitoring oral temperature, heart rate, and respiration rate of West Indian manatees (Trichechus manatus) during capture and handling in the field
West Indian manatees (Trichechus manatus) are captured, handled, and transported to facilitate conservation, research, and rehabilitation efforts. Monitoring manatee oral temperature (OT), heart rate (HR), and respiration rate (RR) during out-of-water handling can assist efforts to maintain animal well-being and improve medical response to evidence of declining health. To determine effects of capt
Authors
Arthur W. Wong, Robert K. Bonde, Jessica Siegal-Willott, M. Andrew Stamper, James Colee, James A. Powell, James P. Reid, Charles J. Deutsch, Kendal E. Harr
Low genetic diversity and minimal population substructure in the endangered Florida manatee: implications for conservation
Species of management concern that have been affected by human activities typically are characterized by low genetic diversity, which can adversely affect their ability to adapt to environmental changes. We used 18 microsatellite markers to genotype 362 Florida manatees (Trichechus manatus latirostris), and investigated genetic diversity, population structure, and estimated genetically effective p
Authors
Kimberly Pause Tucker, Margaret E. Hunter, Robert K. Bonde, James D. Austin, Ann Marie Clark, Cathy A. Beck, Peter M. McGuire, Madan K. Oli
Secretion of anti-Müllerian hormone in the Florida manatee Trichechus manatus latirostris, with implications for assessing conservation status
Environmental and anthropogenic stressors can affect wildlife populations in a number of ways. For marine mammals (e.g. the Florida manatee Trichechus manatus latirostris), certain stressors or conservation risk factors have been identified, but sublethal effects have been very difficult to assess using traditional methods. The development of 'biomarkers' allows us to correlate effects, such as im
Authors
Rhian C. Wilson, John E. Reynolds, Dana L. Wetzel, Leslie Schwierzke-Wade, Robert K. Bonde, Kevin F. Breuel, William E. Roudebush
Seroepidemiology of TmPV1 infection in captive and wild Florida manatees (Trichechus manatus latirostris)
In 1997, cutaneous papillomatosis caused by Florida manatee (Trichechus manatus latirostris [Tm]) papillomavirus 1 (TmPV1) was detected in seven captive manatees at the Homosassa Springs Wildlife State Park, Florida, USA, and, subsequently, in two wild manatees from the adjacent Homosassa River. Since then, papillomatosis has been reported in captive manatees housed in other locations, but not in
Authors
Maria Gabriella Dona, Manuela Rehtanz, Nicole M. Adimey, Gregory D. Bossart, Alfred B. Jenson, Robert K. Bonde, Shin-je Ghim
Evaluation of adrenocortical function in Florida manatees (Trichechus manatus latirostris)
The study objectives were to determine the predominant manatee glucocorticoid; validate assays to measure this glucocorticoid and adrenocorticotropic hormone (ACTH); determine diagnostic thresholds to distinguish physiological vs. pathological concentrations; identify differences associated with sex, age class, female reproductive status, capture time, and lactate; and determine the best methods f
Authors
K.M. Tripp, J.P. Verstegen, C. Deutsch, Robert K. Bonde, M. de Wit, Charles A. Manire, J. Gaspard, K.E. Harr
Serum amyloid A (SAA) as a biomarker of chronic infection due to boat strike trauma in a free-ranging Florida manatee (Trichechus manatus latirostris) with incidental polycystic kidneys
Watercraft-related trauma is the predominant cause of human-induced mortality in manatees (Trichechus manatus latirostris), a federal- and state-listed endangered species. Pyothorax (documented in this case report) and other secondary infections are common sequelae of inhalation of water and the open wounds caused by boat propellers. These secondary infections can lead to the demise of the animal
Authors
Kendal E. Harr, Renee Rember, Pamela E. Ginn, Jessica Lightsey, Martha Keller, James Reid, Robert K. Bonde
Evidence of two genetic clusters of manatees with low genetic diversity in Mexico and implications for their conservation
The Antillean manatee (Trichechus manatus manatus) occupies the tropical coastal waters of the Greater Antilles and Caribbean, extending from Mexico along Central and South America to Brazil. Historically, manatees were abundant in Mexico, but hunting during the pre-Columbian period, the Spanish colonization and throughout the history of Mexico, has resulted in the significantly reduced population
Authors
C. Nourisson, B. Morales-Vela, J. Padilla-Saldivar, K.P. Tucker, A. Clark, L. D. Olivera-Gomez, Robert K. Bonde, P. McGuire
Feeding preferences of West Indian manatees in Florida, Belize, and Puerto Rico as indicated by stable isotope analysis
The endangered West Indian manatee Trichechus manatus has 2 recognized subspecies: the Florida T. m. latirostris and Antillean T. m. manatus manatee, both of which are found in freshwater, estuarine, and marine habitats. A better understanding of manatee feeding preferences and habitat use is essential to establish criteria on which conservation plans can be based. Skin from manatees in Florida, B
Authors
Christy D. Alves-Stanley, Graham A.J. Worthy, Robert K. Bonde
Low genetic variation and evidence of limited dispersal in the regionally important Belize manatee
The Antillean subspecies of the West Indian manatee Trichechus manatus is found throughout Central and South America and the Caribbean. Because of severe hunting pressure during the 17th through 19th centuries, only small populations of the once widespread aquatic mammal remain. Fortunately, protections in Belize reduced hunting in the 1930s and allowed the country's manatee population to become t
Authors
M.E. Hunter, N. E. Auil-Gomez, K.P. Tucker, R. K. Bonde, J. Powell, P.M. McGuire
Physiological response of wild dugongs (Dugong dugon) to out-of-water sampling for health assessment
The dugong (Dugong dugon) is a vulnerable marine mammal with large populations living in urban Queensland waters. A mark-recapture program for wild dugongs has been ongoing in southern Queensland since 2001. This program has involved capture and in-water sampling of more than 700 dugongs where animals have been held at the water surface for 5 min to be gene-tagged, measured, and biopsied. In 2008,
Authors
Janet M. Lanyon, Helen L. Sneath, Trevor Long, Robert K. Bonde
Characterization of highly informative cross-species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers
The Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) are threatened species of aquatic mammals in the order Sirenia. Sirenian conservation and management actions would benefit from a more complete understanding of genetic diversity and population structure. Generally, species-specific microsatellite markers are employed in conservation genetic studies; however,
Authors
Margaret Kellogg Hunter, Damien Broderick, Jennifer R. Ovenden, Kimberly Pause Tucker, Robert K. Bonde, Peter M. McGuire, Janet M. Lanyon
Sexing sirenians: Validation of visual and molecular sex determination in both wild dugongs (Dugong dugon) and Florida manatees (Trichechus manatus latirostris)
Sexing wild marine mammals that show little to no sexual dimorphism is challenging. For sirenians that are difficult to catch or approach closely, molecular sexing from tissue biopsies offers an alternative method to visual discrimination. This paper reports the results of a field study to validate the use of two sexing methods: (1) visual discrimination of sex vs (2) molecular sexing based on a m
Authors
J. Lanyon, H. Sneath, J. Ovenden, D. Broderick, Robert K. Bonde
Science and Products
Filter Total Items: 93
Monitoring oral temperature, heart rate, and respiration rate of West Indian manatees (Trichechus manatus) during capture and handling in the field
West Indian manatees (Trichechus manatus) are captured, handled, and transported to facilitate conservation, research, and rehabilitation efforts. Monitoring manatee oral temperature (OT), heart rate (HR), and respiration rate (RR) during out-of-water handling can assist efforts to maintain animal well-being and improve medical response to evidence of declining health. To determine effects of capt
Authors
Arthur W. Wong, Robert K. Bonde, Jessica Siegal-Willott, M. Andrew Stamper, James Colee, James A. Powell, James P. Reid, Charles J. Deutsch, Kendal E. Harr
Low genetic diversity and minimal population substructure in the endangered Florida manatee: implications for conservation
Species of management concern that have been affected by human activities typically are characterized by low genetic diversity, which can adversely affect their ability to adapt to environmental changes. We used 18 microsatellite markers to genotype 362 Florida manatees (Trichechus manatus latirostris), and investigated genetic diversity, population structure, and estimated genetically effective p
Authors
Kimberly Pause Tucker, Margaret E. Hunter, Robert K. Bonde, James D. Austin, Ann Marie Clark, Cathy A. Beck, Peter M. McGuire, Madan K. Oli
Secretion of anti-Müllerian hormone in the Florida manatee Trichechus manatus latirostris, with implications for assessing conservation status
Environmental and anthropogenic stressors can affect wildlife populations in a number of ways. For marine mammals (e.g. the Florida manatee Trichechus manatus latirostris), certain stressors or conservation risk factors have been identified, but sublethal effects have been very difficult to assess using traditional methods. The development of 'biomarkers' allows us to correlate effects, such as im
Authors
Rhian C. Wilson, John E. Reynolds, Dana L. Wetzel, Leslie Schwierzke-Wade, Robert K. Bonde, Kevin F. Breuel, William E. Roudebush
Seroepidemiology of TmPV1 infection in captive and wild Florida manatees (Trichechus manatus latirostris)
In 1997, cutaneous papillomatosis caused by Florida manatee (Trichechus manatus latirostris [Tm]) papillomavirus 1 (TmPV1) was detected in seven captive manatees at the Homosassa Springs Wildlife State Park, Florida, USA, and, subsequently, in two wild manatees from the adjacent Homosassa River. Since then, papillomatosis has been reported in captive manatees housed in other locations, but not in
Authors
Maria Gabriella Dona, Manuela Rehtanz, Nicole M. Adimey, Gregory D. Bossart, Alfred B. Jenson, Robert K. Bonde, Shin-je Ghim
Evaluation of adrenocortical function in Florida manatees (Trichechus manatus latirostris)
The study objectives were to determine the predominant manatee glucocorticoid; validate assays to measure this glucocorticoid and adrenocorticotropic hormone (ACTH); determine diagnostic thresholds to distinguish physiological vs. pathological concentrations; identify differences associated with sex, age class, female reproductive status, capture time, and lactate; and determine the best methods f
Authors
K.M. Tripp, J.P. Verstegen, C. Deutsch, Robert K. Bonde, M. de Wit, Charles A. Manire, J. Gaspard, K.E. Harr
Serum amyloid A (SAA) as a biomarker of chronic infection due to boat strike trauma in a free-ranging Florida manatee (Trichechus manatus latirostris) with incidental polycystic kidneys
Watercraft-related trauma is the predominant cause of human-induced mortality in manatees (Trichechus manatus latirostris), a federal- and state-listed endangered species. Pyothorax (documented in this case report) and other secondary infections are common sequelae of inhalation of water and the open wounds caused by boat propellers. These secondary infections can lead to the demise of the animal
Authors
Kendal E. Harr, Renee Rember, Pamela E. Ginn, Jessica Lightsey, Martha Keller, James Reid, Robert K. Bonde
Evidence of two genetic clusters of manatees with low genetic diversity in Mexico and implications for their conservation
The Antillean manatee (Trichechus manatus manatus) occupies the tropical coastal waters of the Greater Antilles and Caribbean, extending from Mexico along Central and South America to Brazil. Historically, manatees were abundant in Mexico, but hunting during the pre-Columbian period, the Spanish colonization and throughout the history of Mexico, has resulted in the significantly reduced population
Authors
C. Nourisson, B. Morales-Vela, J. Padilla-Saldivar, K.P. Tucker, A. Clark, L. D. Olivera-Gomez, Robert K. Bonde, P. McGuire
Feeding preferences of West Indian manatees in Florida, Belize, and Puerto Rico as indicated by stable isotope analysis
The endangered West Indian manatee Trichechus manatus has 2 recognized subspecies: the Florida T. m. latirostris and Antillean T. m. manatus manatee, both of which are found in freshwater, estuarine, and marine habitats. A better understanding of manatee feeding preferences and habitat use is essential to establish criteria on which conservation plans can be based. Skin from manatees in Florida, B
Authors
Christy D. Alves-Stanley, Graham A.J. Worthy, Robert K. Bonde
Low genetic variation and evidence of limited dispersal in the regionally important Belize manatee
The Antillean subspecies of the West Indian manatee Trichechus manatus is found throughout Central and South America and the Caribbean. Because of severe hunting pressure during the 17th through 19th centuries, only small populations of the once widespread aquatic mammal remain. Fortunately, protections in Belize reduced hunting in the 1930s and allowed the country's manatee population to become t
Authors
M.E. Hunter, N. E. Auil-Gomez, K.P. Tucker, R. K. Bonde, J. Powell, P.M. McGuire
Physiological response of wild dugongs (Dugong dugon) to out-of-water sampling for health assessment
The dugong (Dugong dugon) is a vulnerable marine mammal with large populations living in urban Queensland waters. A mark-recapture program for wild dugongs has been ongoing in southern Queensland since 2001. This program has involved capture and in-water sampling of more than 700 dugongs where animals have been held at the water surface for 5 min to be gene-tagged, measured, and biopsied. In 2008,
Authors
Janet M. Lanyon, Helen L. Sneath, Trevor Long, Robert K. Bonde
Characterization of highly informative cross-species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers
The Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) are threatened species of aquatic mammals in the order Sirenia. Sirenian conservation and management actions would benefit from a more complete understanding of genetic diversity and population structure. Generally, species-specific microsatellite markers are employed in conservation genetic studies; however,
Authors
Margaret Kellogg Hunter, Damien Broderick, Jennifer R. Ovenden, Kimberly Pause Tucker, Robert K. Bonde, Peter M. McGuire, Janet M. Lanyon
Sexing sirenians: Validation of visual and molecular sex determination in both wild dugongs (Dugong dugon) and Florida manatees (Trichechus manatus latirostris)
Sexing wild marine mammals that show little to no sexual dimorphism is challenging. For sirenians that are difficult to catch or approach closely, molecular sexing from tissue biopsies offers an alternative method to visual discrimination. This paper reports the results of a field study to validate the use of two sexing methods: (1) visual discrimination of sex vs (2) molecular sexing based on a m
Authors
J. Lanyon, H. Sneath, J. Ovenden, D. Broderick, Robert K. Bonde