Robert Bonde, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 93
Hematology of healthy Florida manatees (Trichechus manatus)
Background: Hematologic analysis is an important tool in evaluating the general health status of free-ranging manatees and in the diagnosis and monitoring of rehabilitating animals. Objectives: The purpose of this study was to evaluate diagnostically important hematologic analytes in healthy manatees (Trichechus manatus) and to assess variations with respect to location (free ranging vs captive),
Authors
J. W. Harvey, K.E. Harr, D. Murphy, M.T. Walsh, E.C. Nolan, R. K. Bonde, M.G. Pate, C. J. Deutsch, H.H. Edwards, W.L. Clapp
Conservation: saving Florida's manatees
Robert K. Bonde of the U.S. Geological Survey writes about the protected population of manatees in Crystal River, Florida, including information about the threats they face as they migrate in and out of protected waters. Photographer Carol Grant shares images of "Angel," a newborn manatee she photographed early one winter morning.
Authors
Robert K. Bonde
Validation of a serum immunoassay to measure progesterone and diagnose pregnancy in the West Indian manatee (Trichechus manatus)
The objective was to validate a high-sensitivity chemiluminescent assay of serum progesterone concentrations for pregnancy diagnosis in manatees. Assay analytical sensitivity was 0.1 ng/mL, with mean intra- and inter-assay coefficients of variation of 9.7 and 9.2%, respectively, and accuracy had a mean adjusted R2 of 0.98. Methods comparison (relative to Siemen's Coat-A-Count RIA) demonstrated r =
Authors
K.M. Tripp, J.P. Verstegen, C. J. Deutsch, R. K. Bonde, M. Rodriguez, B. Morales, D.L. Schmitt, K.E. Harr
Concentrations of trace elements in blood and skin of Florida manatees (Trichechus manatus latirostris)
[No abstract available]
Authors
H.-C.W. Stavros, R. K. Bonde, P.A. Fair
Comparison of blood aminotransferase methods for assessment of myopathy and hepatopathy in Florida manatees (Trichechus manatus latirostris)
Muscle injury is common in Florida manatees (Trichechus manatus latirostris). Plasma aspartate amino-transferase (AST) is frequently used to assess muscular damage in capture myopathy and traumatic injury. Therefore, accurate measurement of AST and alanine aminotransferase (ALT) is important in managed, free-ranging animals, as well as in those rehabilitating from injury. Activities of these enzym
Authors
K.E. Harr, K. Allison, R. K. Bonde, D. Murphy, J. W. Harvey
Procedures for the salvage and necropsy of the dugong (Dugong dugon)
Data and specimens collected from dugong carcasses and live stranded individuals provide vital information for research and management agencies. The ability to assign a cause of death (natural and/or human induced) to a carcass assists managers to identify major threats to a population in certain areas and to evaluate and adapt management measures. Data collectedfrom dugong carcasses have contribu
Authors
Carole Eros, Helene Marsh, Robert K. Bonde, Thomas A. O'Shea, Cathy A. Beck, Cheri Recchia, Kirstin Dobbs, Malcolm Turner, Stephanie Lemm, Rachel Pears, Rachel Bowater
Polymorphic microsatellite DNA markers for the Florida manatee (Trichechus manatus latirostris)
Florida manatees (Trichechus manatus latirostris) are marine mammals that inhabit the coastal waters and rivers of the southeastern USA, primarily Florida. Previous studies have shown that Florida manatees have low mitochondrial DNA variability, suggesting that nuclear DNA loci are necessary for discriminatory analyses. Here we report 10 polymorphic microsatellite loci with an average of 4.2 allel
Authors
K.C. Pause, C. Nourisson, A. Clark, M.E. Kellogg, R. K. Bonde, P.M. McGuire
Chromosome painting among Proboscidea, Hyracoidea and Sirenia: Support for Paenungulata (Afrotheria, Mammalia) but not Tethytheria
Despite marked improvements in the interpretation of systematic relationships within Eutheria, particular nodes, including Paenungulata (Hyracoidea, Sirenia and Proboscidea), remain ambiguous. The combination of a rapid radiation, a deep divergence and an extensive morphological diversification has resulted in a limited phylogenetic signal confounding resolution within this clade both at the morph
Authors
A.T. Pardini, P. C. M. O'Brien, B. Fu, R. K. Bonde, F.F.B. Elder, M. A. Ferguson-Smith, F. Yang, T.J. Robinson
Clinical biochemistry in healthy manatees (Trichechus manatus latirostris)
Florida manatees (Trichechus manatus latirostris) are endangered aquatic mammals living in coastal and riverine waterways of Florida and adjacent states. Serum or plasma biochemical analyses are important tools in evaluating the health of free-ranging and captive manatees. The purpose of this study was to measure diagnostically important analytes in the plasma of healthy manatees and to determine
Authors
J. W. Harvey, K.E. Harr, D. Murphy, M.T. Walsh, E.J. Chittick, R. K. Bonde, M.G. Pate, C. J. Deutsch, H.H. Edwards, E.M. Haubold
Right whale mortality: a message from the dead to the living
Abstract not supplied at this time
Authors
M.J. Moore, W.A. McLellan, P.-Y. Daoust, R. K. Bonde, A.R. Knowlton
A comparison of biofouling communities associated with free-ranging and captive Florida manatees (Trichechus manatus manatus)
No abstract available.
Authors
Erin L. Bledsoe, Kendal E. Harr, Mary F. Cichra, Edward J. Philips, Robert K. Bonde, Mark Lowe
Science and Products
Filter Total Items: 93
Hematology of healthy Florida manatees (Trichechus manatus)
Background: Hematologic analysis is an important tool in evaluating the general health status of free-ranging manatees and in the diagnosis and monitoring of rehabilitating animals. Objectives: The purpose of this study was to evaluate diagnostically important hematologic analytes in healthy manatees (Trichechus manatus) and to assess variations with respect to location (free ranging vs captive),
Authors
J. W. Harvey, K.E. Harr, D. Murphy, M.T. Walsh, E.C. Nolan, R. K. Bonde, M.G. Pate, C. J. Deutsch, H.H. Edwards, W.L. Clapp
Conservation: saving Florida's manatees
Robert K. Bonde of the U.S. Geological Survey writes about the protected population of manatees in Crystal River, Florida, including information about the threats they face as they migrate in and out of protected waters. Photographer Carol Grant shares images of "Angel," a newborn manatee she photographed early one winter morning.
Authors
Robert K. Bonde
Validation of a serum immunoassay to measure progesterone and diagnose pregnancy in the West Indian manatee (Trichechus manatus)
The objective was to validate a high-sensitivity chemiluminescent assay of serum progesterone concentrations for pregnancy diagnosis in manatees. Assay analytical sensitivity was 0.1 ng/mL, with mean intra- and inter-assay coefficients of variation of 9.7 and 9.2%, respectively, and accuracy had a mean adjusted R2 of 0.98. Methods comparison (relative to Siemen's Coat-A-Count RIA) demonstrated r =
Authors
K.M. Tripp, J.P. Verstegen, C. J. Deutsch, R. K. Bonde, M. Rodriguez, B. Morales, D.L. Schmitt, K.E. Harr
Concentrations of trace elements in blood and skin of Florida manatees (Trichechus manatus latirostris)
[No abstract available]
Authors
H.-C.W. Stavros, R. K. Bonde, P.A. Fair
Comparison of blood aminotransferase methods for assessment of myopathy and hepatopathy in Florida manatees (Trichechus manatus latirostris)
Muscle injury is common in Florida manatees (Trichechus manatus latirostris). Plasma aspartate amino-transferase (AST) is frequently used to assess muscular damage in capture myopathy and traumatic injury. Therefore, accurate measurement of AST and alanine aminotransferase (ALT) is important in managed, free-ranging animals, as well as in those rehabilitating from injury. Activities of these enzym
Authors
K.E. Harr, K. Allison, R. K. Bonde, D. Murphy, J. W. Harvey
Procedures for the salvage and necropsy of the dugong (Dugong dugon)
Data and specimens collected from dugong carcasses and live stranded individuals provide vital information for research and management agencies. The ability to assign a cause of death (natural and/or human induced) to a carcass assists managers to identify major threats to a population in certain areas and to evaluate and adapt management measures. Data collectedfrom dugong carcasses have contribu
Authors
Carole Eros, Helene Marsh, Robert K. Bonde, Thomas A. O'Shea, Cathy A. Beck, Cheri Recchia, Kirstin Dobbs, Malcolm Turner, Stephanie Lemm, Rachel Pears, Rachel Bowater
Polymorphic microsatellite DNA markers for the Florida manatee (Trichechus manatus latirostris)
Florida manatees (Trichechus manatus latirostris) are marine mammals that inhabit the coastal waters and rivers of the southeastern USA, primarily Florida. Previous studies have shown that Florida manatees have low mitochondrial DNA variability, suggesting that nuclear DNA loci are necessary for discriminatory analyses. Here we report 10 polymorphic microsatellite loci with an average of 4.2 allel
Authors
K.C. Pause, C. Nourisson, A. Clark, M.E. Kellogg, R. K. Bonde, P.M. McGuire
Chromosome painting among Proboscidea, Hyracoidea and Sirenia: Support for Paenungulata (Afrotheria, Mammalia) but not Tethytheria
Despite marked improvements in the interpretation of systematic relationships within Eutheria, particular nodes, including Paenungulata (Hyracoidea, Sirenia and Proboscidea), remain ambiguous. The combination of a rapid radiation, a deep divergence and an extensive morphological diversification has resulted in a limited phylogenetic signal confounding resolution within this clade both at the morph
Authors
A.T. Pardini, P. C. M. O'Brien, B. Fu, R. K. Bonde, F.F.B. Elder, M. A. Ferguson-Smith, F. Yang, T.J. Robinson
Clinical biochemistry in healthy manatees (Trichechus manatus latirostris)
Florida manatees (Trichechus manatus latirostris) are endangered aquatic mammals living in coastal and riverine waterways of Florida and adjacent states. Serum or plasma biochemical analyses are important tools in evaluating the health of free-ranging and captive manatees. The purpose of this study was to measure diagnostically important analytes in the plasma of healthy manatees and to determine
Authors
J. W. Harvey, K.E. Harr, D. Murphy, M.T. Walsh, E.J. Chittick, R. K. Bonde, M.G. Pate, C. J. Deutsch, H.H. Edwards, E.M. Haubold
Right whale mortality: a message from the dead to the living
Abstract not supplied at this time
Authors
M.J. Moore, W.A. McLellan, P.-Y. Daoust, R. K. Bonde, A.R. Knowlton
A comparison of biofouling communities associated with free-ranging and captive Florida manatees (Trichechus manatus manatus)
No abstract available.
Authors
Erin L. Bledsoe, Kendal E. Harr, Mary F. Cichra, Edward J. Philips, Robert K. Bonde, Mark Lowe