Droplet digital PCR data for the detection of Smoky Mountain Madtoms in Great Smoky Mountains National Park

Environmental DNA water samples were collected at multiple sites in Great Smoky Mountains National Park in 2016 and 2017 and analyzed with a droplet digital PCR assay to screen for the presence of smoky mountain madtoms. Smoky Mountain madtoms were detected within a section of Abrams Creek, known as the only stream in Great Smoky Mountains National Park that supports this species.