Fig. 2. Nonlinear regression prediction plot for use with 50 pooled l

Fig. 2. Nonlinear regression prediction plot for use with 50 pooled larvae that accurately estimates the percentage of triploids in a mixed population of triploids and diploids of that spawn. The plot was generated from 10 replicate experiments, each using a FCM protocol for cell processing and analysis at 15 known levels of ploidy. The 95% confidence limits (black dashed lines) and mean values (dots), along with the 95% prediction curves (red dashed lines) are shown. The corresponding quadratic equation is above. Thus, a flow cytometric-derived observed value (y–axis) (within the range) will correspond to a predicted triploidy value (x-axis) for a spawn.