Summary of water chemistry and male largemouth bass (LMB; Micropterus salmoides) reproductive condition

Summary of water chemistry and male largemouth bass (LMB; Micropterus salmoides) reproductive condition: (A,B) concentrations of estrone (E1; green = concentration in control pond, red = concentration in dosed ponds) and atrazine (ATR; green = concentration in control pond, blue = concentration in dosed ponds) as measured in the mixture and control ponds at each weekly sampling and averaged across ponds (n = 3); (C) concentration of 17alpha-ethinylestradiol (EE2) at each weekly sampling across ponds and averaged across ponds (n = 3); (D) mean gonadosomatic index (GSI; ± standard error) of male LMB at the four timepoints (see Table S1 for n), different letters denote significant differences (p < 0.05); (E) percent total sperm motility (solid bars) and percent progressive sperm motility (hashed bars) at the April time point (Control n = 12, E1 + ATR n = 11, EE2 n = 15), just prior to spawning, asterisks denote significant differences compared to control (p < 0.05); (F) sperm count (cell count per mL milt) at the April time point (Control n = 12, E1 + ATR n = 11, EE2 n = 10), just prior to spawning, asterisk denotes a significant difference compared to control (p < 0.05).