More than 50 feedstuffs used or of potential use in diets for rainbow trout Oncorhyndus mykiss were compared through metabolism chamber trials. Testing included determination of apparent digestibility and metabolic energy for a wide variety of ingredients, including fish and animal meals, vegetable proteins, and single cell protein products. Testing involved feeding individual 200–300-g fish contained in replicate chambers, from which feces, urine, and gill excreta were collected seg arately and analyzed for residual nitrogen and energy. For comparison, several products previously tested at this or other laboratories were re-evaluated at dietary concentrations of 25, 50, or 100%.