Isolation of a reovirus from coho salmon (Oncorhynchus kisutch) in Oregon, USA
Reoviruses isolated from aquatic animals share certain common characteristics: (1) a typical reovirus-like morphology which shows an icosahedral particle with a double capsid that is approximately 75 nm in diameter; (2) a genome with eleven segments of double-stranded RNA (dsRNA) distributed as three large, three medium and five small segments with a total molecular weight of approximately 15 x 106; (3) a virion composed of five major and several minor structural proteins that range in molecular weight from 32,000 to 137,000; and (4) form plaque-like syncytia in monolayer cultures of fish cells. Intact virus particles have buoyant densities in CsCl of 1.34 to 1.36 g/ml. The viruses have been isolated from fish and shellfish collected in both the marine and freshwater environments and will replicate in several fish cell lines (Plumb et al., 1979; Meyers and Hirai, 1980; Winton et al., 1981; Nagabayashi and Mori, 1983; Hedrick et al., 1984; Chen and Jiang, 1984). The original four aquatic reovirus isolates have been compared by Winton et al., 1987.
Citation Information
Publication Year | 1989 |
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Title | Isolation of a reovirus from coho salmon (Oncorhynchus kisutch) in Oregon, USA |
Authors | J. R. Winton, C.N. Arakawa, C.N. Lannan, J. L. Fryer |
Publication Type | Book Chapter |
Publication Subtype | Book Chapter |
Index ID | 70162024 |
Record Source | USGS Publications Warehouse |
USGS Organization | Western Fisheries Research Center |