Morphometric differentiation of American shad and white sucker eggs from riverine samples

We developed a statistical method to distinguish the large demersal eggs of American shad from those of white sucker in riverine samples using egg morphometric analysis. Eggs were first screened by total diameter in deionized water according to ranges reported in the published literature. Differences in relative yolk diameter between the two species were then determined statistically from known museum sources. Only those eggs with relative yolk diameters greater than two standard deviations below the mean for white sucker eggs were considered to be American shad eggs. The criteria for American shad eggs were total diameter ≥2.3 nm and relative yolk diameter ≤66%. A partial test of the model showed predicted identity to agree with observed identity for 74 out of 75 shad eggs.