TanA: a fluorogenic probe for thiaminase activity
January 1, 2013
A fluorogenic thiamine analogue is presented as a fluorescent probe for thiaminase activity. The emission of the fluorophore is quenched by photoinduced electron transfer (PET) to the N-substituted pyridinium portion of the probe. Action of the enzyme releases the free pyridine group causing a substantial increase in fluorescence.
Citation Information
Publication Year | 2013 |
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Title | TanA: a fluorogenic probe for thiaminase activity |
DOI | 10.1039/C2AY26047F |
Authors | Wanjun Zhu, James L. Zajicek, Donald E. Tillitt, Timothy E. Glass |
Publication Type | Article |
Publication Subtype | Journal Article |
Series Title | Analytical Methods |
Index ID | 70048566 |
Record Source | USGS Publications Warehouse |
USGS Organization | Columbia Environmental Research Center |