Novel protocol for moose DNA extraction from Tempus blood tube supernatant following RNA extraction
July 24, 2018
Data were collected to validate the use of supernatant from the isolation of RNA from Tempus blood RNA tubes to obtain genomic DNA in the associated blood sample from moose. Data represent the metrics measured to validate the novel technique for isolating DNA from the supernatant of an RNA isolation protocol using blood collected into Tempus blood RNA tubes.
Citation Information
| Publication Year | 2018 |
|---|---|
| Title | Novel protocol for moose DNA extraction from Tempus blood tube supernatant following RNA extraction |
| DOI | 10.5066/P9DWV93U |
| Authors | Michelle Renee Giles, Margaret (Maggie) E. Hunter, Jason A Ferrante, Emily Benzie |
| Product Type | Data Release |
| Record Source | USGS Asset Identifier Service (AIS) |
| USGS Organization | Wetland and Aquatic Research Center - Gainesville, FL |
| Rights | This work is marked with CC0 1.0 Universal |
Related
A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
ObjectiveWe use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration...
Authors
Jason A. Ferrante, Michelle R. Giles, Emily Benzie, Margaret E. Hunter
Related
A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
ObjectiveWe use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration...
Authors
Jason A. Ferrante, Michelle R. Giles, Emily Benzie, Margaret E. Hunter