Tree-ring reconstructions of water-year (Oct 1 through Sep 30th) flow for 31 gaging sites in Missouri River basin, with complete data for 1685 through 1977 (n = 293 water years). The complete 105 tree-ring reconstructions of streamflow used in the Nature Scientific Reports paper were obtained from various sources; 74 flow reconstructions were obtained from the web resource, TreeFlow (http://www.treeflow.info/), and an additional 31 flow reconstructions (provided here) for the Missouri River basin were obtained from the U.S. Geological Survey in Bozeman, Montana.