Plain pocketbook (Lampsilis cardium) glochidia counts and transformation rates collected during laboratory exposures to agriculture and urban contaminant mixtures and measured contaminant concentrations, 2018

This data release includes datasets provided by the Woolnough Laboratory at Central Michigan University. These data were collected as a part of a laboratory exposure experiment, conducted in 2018, examining the effects of agricultural and urban contaminant mixtures on two life stages (larval and juvenile) of the freshwater mussel, plain pocketbook (Lampsilis cardium), using largemouth bass (Micropterus salmoides) as the host fish. Contaminant mixtures were representative of contaminants frequently detected in agricultural and urban settings. Agricultural contaminant mixtures consisted of bromacil, estrone, metolachlor, tri(butoxyethyl) phosphate, 4-nonylphenol, atrazine, N,N-diethyl-meta-toluamide (DEET), and bisphenol A. Urban contaminant mixtures consisted of metformin, sulfamethoxazole, desvenlafazine, and 5-methyl-1H-benzotriazole. Lampsilis cardium and M. salmoides were exposed to either an agricultural or urban contaminant mixture, control water treatment, or control ethanol treatment for 40 or 100 days. Throughout the exposure periods, water samples were collected from the contaminant mixture holding tanks for verification of concentration dosing. Gravid adult female L. cardium and M. salmoides were exposed to a specified treatment for 13 or 60 days. During this exposure period, prematurely released glochidia (L. cardium larvae) were enumerated. After the initial exposure period, glochidia were stripped from gravid female L. cardium, enumerated, and inoculated into M. salmoides tanks of the corresponding treatment for infestation and encystment. After infestation, parasitic glochidia and M. salmoides host fish were exposed for 27 or 40 more days (40- and 100-day total exposure durations, respectively). During this exposure period, glochidia that dropped off the host fish were enumerated and observations of their metamorphose state (partially or fully metamorphosed) were documented. The data contained in this data release were analyzed and discussed in the primary publication titled “Reduced freshwater mussel juvenile production as a result of agricultural and urban contaminant mixture exposures” (https://doi.org/10.1002/etc.5844).