Strontium isotope ratios of lavas from Hawaii were analyzed by thermal ionization mass spectrometry (TIMS) at the Southwest Isotope Research Laboratories of the U.S. Geological Survey (USGS) in Denver. There were at total of 427 analyses of samples obtained from the field, the collections of the USGS Hawaiian Volcano Observatory (HVO), the University of Hawai?i, and the Smithsonian Institution. The samples originated from Kilauea (historical and prehistoric summit and rift zone lavas), Mauna Loa (historical lavas), and Loihi (submarine lavas of unknown age). Data for associated reference materials are described in the process steps. The Sr isotope ratios may be used to test models for the magmatic plumbing system of each volcano. This data release supersedes the following data release: Pietruszka, A.J., 2019, Strontium isotope ratios of lavas from Kilauea Volcano, Hawaii: U.S. Geological Survey data release, https://doi.org/10.5066/P9YMNIAT.