Caitlin Beaver (WARC) setting up plates to run ddPCR in the Biosafety-level 3 lab in Leetown, WV.
Images
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ddPCR Lab
Caitlin Beaver (WARC) setting up plates to run ddPCR in the Biosafety-level 3 lab in Leetown, WV.
Composite Wastewater Sample
Image of a flow-based composite sample being collected at one of the wastewater facilities to represent a 24-hour sample.
Image of a flow-based composite sample being collected at one of the wastewater facilities to represent a 24-hour sample.
Paperwork
Picture of Travis Smith at a wastewater facility filling out paperwork for samples collected that day in the lab van before shipping the same samples to the Leetown, WV lab.
Picture of Travis Smith at a wastewater facility filling out paperwork for samples collected that day in the lab van before shipping the same samples to the Leetown, WV lab.
Retrieving a Sample
A USGS field team member retrieving the sample bottle from a time-based sampler onsite at a wastewater treatment facility.
A USGS field team member retrieving the sample bottle from a time-based sampler onsite at a wastewater treatment facility.
Wisconsin Sample Collection
Field team member Laura Hubbard shows off a sample in the field after pickup from the wastewater facility.
Field team member Laura Hubbard shows off a sample in the field after pickup from the wastewater facility.
Early Morning Sample Collection
Red biohazard bag, sealed bags with samples in bottles and a sample box on the ground.
Red biohazard bag, sealed bags with samples in bottles and a sample box on the ground.
RPS
Julie Dietze vs. Jonathon Cohl in a Rock/Paper/Scissors showdown that you'd have to see to believe!
Julie Dietze vs. Jonathon Cohl in a Rock/Paper/Scissors showdown that you'd have to see to believe!
Logistics Team Members
Justin Krahulik, Logan Neal, Moussu Guira & Julie Dietze finish packing the last shipments for the field.
Justin Krahulik, Logan Neal, Moussu Guira & Julie Dietze finish packing the last shipments for the field.
Sample Pickup
Rachel Atkins retrieving and packaging samples from each facility, which must be stored on ice during shipment to the Leetown, WV lab
Rachel Atkins retrieving and packaging samples from each facility, which must be stored on ice during shipment to the Leetown, WV lab
Spike of RHV virus
Spike of RHV virus as extraction control in the Biosafety Level-3 Lab. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.
Spike of RHV virus as extraction control in the Biosafety Level-3 Lab. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.
Processing Filtered Samples
1x filtered samples processed via vacuum manifold RNA column filtration. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.
1x filtered samples processed via vacuum manifold RNA column filtration. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.
Transfer or RNA Spin Columns 1
Transfer of RNA spin columns into collection tubes (with Chris Ottinger and Colleen Young)
Transfer of RNA spin columns into collection tubes (with Chris Ottinger and Colleen Young)
Transfer or RNA Spin Columns 2
Transfer of RNA spin columns into collection tubes (also pictured Chris Ottinger and Colleen Young)
Transfer of RNA spin columns into collection tubes (also pictured Chris Ottinger and Colleen Young)
PPE donning 1
Luke Iwanowicz dons multiple layers of PPE for safety prior to entering the BSL-3 lab
Luke Iwanowicz dons multiple layers of PPE for safety prior to entering the BSL-3 lab
PPE donning 2
Luke Iwanowicz dons multiple layers of PPE for safety prior to entering the BSL-3 lab
Luke Iwanowicz dons multiple layers of PPE for safety prior to entering the BSL-3 lab
Delivered Sample Boxes at the Lab
Accumulation of post-delivery Sample Boxes in the recieving area of the Leetown facility
Accumulation of post-delivery Sample Boxes in the recieving area of the Leetown facility
Unloading boxes from Fed Ex
Unloading sample boxes from multiple facilities off the Fed Ex Truck, Christine Densmore (front) Robin Johnson (rear)
Unloading sample boxes from multiple facilities off the Fed Ex Truck, Christine Densmore (front) Robin Johnson (rear)
Boxes from Fed Ex
Unloading sample boxes from Fed Ex Truck into the receiving area at the Leetown Lab, Christine Densmore
Unloading sample boxes from Fed Ex Truck into the receiving area at the Leetown Lab, Christine Densmore
RNA Extractions
Luke Iwanowicz (left) Christine Densmore (right) perform RNA extractions in the Biosafety Level-3 lab
Luke Iwanowicz (left) Christine Densmore (right) perform RNA extractions in the Biosafety Level-3 lab
Purification of extracted RNA 1
Purification of extracted RNA via vacuum manifold RNA column filtration in the BSL3 lab.
Purification of extracted RNA via vacuum manifold RNA column filtration in the BSL3 lab.
Deb in the Lab
Deb Iwanowicz grabs a rare selfie from a plastic-encased phone the BSL3 lab.
Deb Iwanowicz grabs a rare selfie from a plastic-encased phone the BSL3 lab.