Michael Eackles (Former Employee)
Science and Products
Filter Total Items: 18
Microsatellite marker development from next-generation sequencing in the New England cottontail (Sylvilagus transitionalis) and cross-amplification in the eastern cottontail (S. floridanus)
ObjectiveThe New England cottontail (Sylvilagus transitionalis) is a species of high conservation priority in the Northeastern United States, and was a candidate for federal listing under the Endangered Species Act until a recent decision determined that conservation actions were sufficient to preclude listing. The aim of this study was to develop a suite of microsatellite loci to guide future res
Authors
Tim L. King, Michael S. Eackles, Aaron Aunins, Thomas J. Jr. McGreevy, Thomas P. Husband, Anthony Tur, Adrienne I. Kovach
The Pleurobemini (Bivalvia: Unionida) revisited: Molecular species delineation using a mitochondrial DNA gene reveals multiple conspecifics and undescribed species
The Pleurobemini (Bivalvia: Unionida) represent approximately one-third of freshwater mussel diversity in North America. Species identification within this group is challenging due to morphological convergence and phenotypic plasticity. Accurate species identification, including characterisation of currently unrecognised taxa, is required to develop effective conservation strategies because many s
Authors
Kentaro Inoue, David M. Hayes, John L. Harris, Nathan A. Johnson, Cheryl L. Morrison, Michael S. Eackles, Tim King, Jess W. Jones, Eric M. Hallerman, Alan D. Christian, Charles R. Randklev
Characterization of 13 microsatellite loci for the deep-sea coral, Lophelia pertusa (Linnaeus 1758), from the western North Atlantic Ocean and Gulf of Mexico
A suite of 13 polymorphic tri- and tetranucleotide microsatellite loci were isolated from the ahermatypic deep-sea coral, Lophelia pertusa. Among 51 individuals collected from three disjunct oceanic regions, allelic diversity ranged from six to 38 alleles and averaged 9.1 alleles per locus. Observed heterozygosity ranged from 9.1 to 96.8% and averaged 62.3% in the Gulf of Mexico population. For so
Authors
C.L. Morrison, M.S. Eackles, Robin L. Johnson, T.L. King
Comprehensive genetic analyses reveal evolutionary distinction of a mouse (Zapus hudsonius preblei) proposed for delisting from the US Endangered Species Act
Zapus hudsonius preblei, listed as threatened under the US Endangered Species Act (ESA), is one of 12 recognized subspecies of meadow jumping mice found in North America. Recent morphometric and phylogenetic comparisons among Z. h. preblei and neighbouring conspecifics questioned the taxonomic status of selected subspecies, resulting in a proposal to delist the Z. h. preblei from the ESA. We prese
Authors
Tim L. King, John F. Switzer, Cheryl L. Morrison, Michael S. Eackles, Colleen Young, Barbara A. Lubinski, Paul M. Cryan
Molecular identification of cypripedioid orchids in international trade
Two cypripedioid orchid genera, Paphiopedilum and Phragmipedium, are listed in Appendix I of CITES and are restricted from international trade. Because of their morphological similarity to other genera, however, they may be disguised as belonging to one of the other cypripedioids listed along with other orchids in Appendix II of CITES. Sequence analysis was performed on the internal transcribed s
Authors
Cheryl Morrison, Katie Hovatter, Michael S. Eackles, A.P. Spidle, T.L. King
Mitochondrial DNA diversity North American and European Atlantic salmon with emphasis on the downeast rivers of Maine
The displacement loop and NADH-1 dehydrogenase regions of mitochondrial DNA (mtDNA) were amplified by the polymerase chain reaction in 954 Atlantic salmon and digested with 40 restriction endonucleases. Variation was detected with 10 enzymes, resulting in 21 composite haplotypes which were strongly patterned geographically with a major discontinuity observed between most North American (NA) and Eu
Authors
T.L. King, A.P. Spidle, M.S. Eackles, B.A. Lubinski, W. B. Schill
Science and Products
Filter Total Items: 18
Microsatellite marker development from next-generation sequencing in the New England cottontail (Sylvilagus transitionalis) and cross-amplification in the eastern cottontail (S. floridanus)
ObjectiveThe New England cottontail (Sylvilagus transitionalis) is a species of high conservation priority in the Northeastern United States, and was a candidate for federal listing under the Endangered Species Act until a recent decision determined that conservation actions were sufficient to preclude listing. The aim of this study was to develop a suite of microsatellite loci to guide future res
Authors
Tim L. King, Michael S. Eackles, Aaron Aunins, Thomas J. Jr. McGreevy, Thomas P. Husband, Anthony Tur, Adrienne I. Kovach
The Pleurobemini (Bivalvia: Unionida) revisited: Molecular species delineation using a mitochondrial DNA gene reveals multiple conspecifics and undescribed species
The Pleurobemini (Bivalvia: Unionida) represent approximately one-third of freshwater mussel diversity in North America. Species identification within this group is challenging due to morphological convergence and phenotypic plasticity. Accurate species identification, including characterisation of currently unrecognised taxa, is required to develop effective conservation strategies because many s
Authors
Kentaro Inoue, David M. Hayes, John L. Harris, Nathan A. Johnson, Cheryl L. Morrison, Michael S. Eackles, Tim King, Jess W. Jones, Eric M. Hallerman, Alan D. Christian, Charles R. Randklev
Characterization of 13 microsatellite loci for the deep-sea coral, Lophelia pertusa (Linnaeus 1758), from the western North Atlantic Ocean and Gulf of Mexico
A suite of 13 polymorphic tri- and tetranucleotide microsatellite loci were isolated from the ahermatypic deep-sea coral, Lophelia pertusa. Among 51 individuals collected from three disjunct oceanic regions, allelic diversity ranged from six to 38 alleles and averaged 9.1 alleles per locus. Observed heterozygosity ranged from 9.1 to 96.8% and averaged 62.3% in the Gulf of Mexico population. For so
Authors
C.L. Morrison, M.S. Eackles, Robin L. Johnson, T.L. King
Comprehensive genetic analyses reveal evolutionary distinction of a mouse (Zapus hudsonius preblei) proposed for delisting from the US Endangered Species Act
Zapus hudsonius preblei, listed as threatened under the US Endangered Species Act (ESA), is one of 12 recognized subspecies of meadow jumping mice found in North America. Recent morphometric and phylogenetic comparisons among Z. h. preblei and neighbouring conspecifics questioned the taxonomic status of selected subspecies, resulting in a proposal to delist the Z. h. preblei from the ESA. We prese
Authors
Tim L. King, John F. Switzer, Cheryl L. Morrison, Michael S. Eackles, Colleen Young, Barbara A. Lubinski, Paul M. Cryan
Molecular identification of cypripedioid orchids in international trade
Two cypripedioid orchid genera, Paphiopedilum and Phragmipedium, are listed in Appendix I of CITES and are restricted from international trade. Because of their morphological similarity to other genera, however, they may be disguised as belonging to one of the other cypripedioids listed along with other orchids in Appendix II of CITES. Sequence analysis was performed on the internal transcribed s
Authors
Cheryl Morrison, Katie Hovatter, Michael S. Eackles, A.P. Spidle, T.L. King
Mitochondrial DNA diversity North American and European Atlantic salmon with emphasis on the downeast rivers of Maine
The displacement loop and NADH-1 dehydrogenase regions of mitochondrial DNA (mtDNA) were amplified by the polymerase chain reaction in 954 Atlantic salmon and digested with 40 restriction endonucleases. Variation was detected with 10 enzymes, resulting in 21 composite haplotypes which were strongly patterned geographically with a major discontinuity observed between most North American (NA) and Eu
Authors
T.L. King, A.P. Spidle, M.S. Eackles, B.A. Lubinski, W. B. Schill