A method of shipping live larvae of Simulium vittatum long distances (Diptera: Simuliidae)

During studies on the biology and ecology of black flies (Simuliidae) at the Seney National Wildlife Refuge, Seney, Michigan, and at the Patuxent Wildlife Research Center, Laurel, Maryland, larvae of 3 species of black fly (Simulium vittatum Zetterstedt, S. aureum Fries, and S. decorum Walker) were collected and transported for distances of up to 50 miles in nonagitated jars of water. In each case the water used to transport the larvae was removed from the waterway where the particular larvae were taken. However, on numerous occasions it was found that larvae could not be kept alive in nonagitated water jars for more than 6 hr.