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Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads

December 31, 2014

We characterized 22 novel microsatellite loci in the two-spotted octopus Octopus bimaculatus using 454 pyrosequencing reads. All loci were polymorphic and will be used in studies of marine connectivity aimed at increasing sustainability of the resource. The mean number alleles per locus was 13.09 (range 7–19) and observed heterozygosities ranged from 0.50 to 1.00. Four loci pairs were linked and three deviated from Hardy–Weinberg equilibrium. Eighteen and 12 loci were polymorphic in Octopus bimaculoides and Octopus hubbsorum, respectively.

Publication Year 2014
Title Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads
DOI 10.1007/s12686-013-0128-x
Authors J. F. Domínguez-Contreras, A. Munguía-Vega, B. P. Ceballos-Vázquez, M. Arellano-Martínez, Melanie Culver
Publication Type Article
Publication Subtype Journal Article
Series Title Conservation Genetics Resources
Index ID 70190492
Record Source USGS Publications Warehouse
USGS Organization Coop Res Unit Seattle