Comparative rice seed toxicity tests using filter paper, growth pouch-tm, and seed tray methods

Paper substrate, especially circular filter paper placed inside a Petri dish, has long been used for the plant seed toxicity test (PSTT). Although this method is simple and inexpensive, recent evidence indicates that it gives results that are significantly different from those obtained using a method that does not involve paper, especially when testing metal cations. The study compared PSTT using three methods: filter paper, Growth Pouch-TM, and seed tray. The Growth Pouch-TM is a commercially available device. The seed tray is a newly designed plastic receptacle placed inside a Petri dish. The results of the Growth Pouch-TM method showed no toxic effects on rice for Ag up to 40 mg L⁻¹ and Cd up to 20 mg L⁻¹. Using the seed tray method, IC₅₀ (50% inhibitory effect concentration) values were 0.55 and 1.4 mg L⁻¹ for Ag and Cd, respectively. Although results of filter paper and seed tray methods were nearly identical for NaF, Cr(VI), and phenol, the toxicities of cations Ag and Cd were reduced by using the filter paper method; IC₅₀ values were 22 and 18 mg L⁻¹, respectively. The results clearly indicate that paper substrate is not advisable for PSTT.