A 180-km-long seismic refraction transect from the eastern High Cascades, across Newberry Volcano, to the eastern High Lava Plains is used to investigate the subvolcanic crustal and upper mantle velocity structure there. Near-surface volcanic flows and sedimentary debris (1.6-4.7 km/s), ranging from 3 to 5 km in thickness, overlie subvolcanic Basin and Range structures. East and west of Newberry Volcano, the subvolcanic basement (5.6 km/s) has been downwarped, producing 5-km-deep basins. The midcrust (8- to 28-km depth) is characterized by velocities ranging from 6.1 to 6.5 km/s and varies laterally in thicknesses. The lower crust is characterized by an unusually high velocity (about 7.4 km/s), and its geometry mirrors the subvolcanic basement geometry. The Moho is located at a depth of 37 km and represents a transition to an upper mantle velocity of 8.1 km/s. The shallow subsurface (1.2 km) beneath Newberry Volcano is characterized by high-velocity 5.6 km/s, versus 4.1 km/s for the surrounding area) intrusions and appears to be located on a basement high. Beneath the seismic refraction array at Newberry Volcano, an absence of low-velocity anomalies suggests that large silicic magma chambers do not exist in the upper crust, but apparent high attenuation of the seismic wave field may be consistent with either partial melts in small volumes, elevated crustal temperatures, and/or poor geophone-recording site coupling.