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Determination of buoyant density and sensitivity to chloroform and freon for the etiological agent of infectious salmonid anaemia

April 29, 1993

Plasma was collected from Atlantic salmon Salrno salar with acute infectious salmon anaemia (ISA) and used to challenge Atlantic salmon parr by intraperitoneal injection. Treatment of plasma with the lipid solvent, chloroform, showed that the etiological agent of ISA contained essential lipids, probably as a viral envelope. Some infectivity remained following treatment with freon. Injection challenges using fractions from equilibrium density gradient centrifugation of plasma from fish with acute ISA revealed a band of infectivity in the range 1.184 to 1.262 g cm-3. The band was believed to conta~n both complete ISA-virus particles and infectious particles lacking a complete envelope, nucleocapsid or genome. Density gradient centrifugation of infectious plasma for enrichment of the putative ISA virus appeared to offer a suitable method for obtaining virus-specific nucleic acid for use in the construction of cDNA libraries. 

Publication Year 1993
Title Determination of buoyant density and sensitivity to chloroform and freon for the etiological agent of infectious salmonid anaemia
DOI 10.3354/dao015225
Authors K.E. Christie, B. Hjeltnes, I. Uglenes, J. R. Winton
Publication Type Article
Publication Subtype Journal Article
Series Title Diseases of Aquatic Organisms
Index ID 70180467
Record Source USGS Publications Warehouse
USGS Organization Western Fisheries Research Center