This report, prepared in cooperation with the West Virginia Office of Federal-State Relations (now the Office of Economic and Community Development), lists in tabular form 435 drainage areas for basins within the Guyandotte River basin of West Virginia. Drainage areas are compiled for sites at the mouths of all streams having drainage areas of approximately five square miles or greater, for sites at U.S. Geological Survey gaging stations (past and present), and for other miscellaneous sites. Drainage areas are summed in a downstream direction to provide areas for main channel sites. The site or reference point of each basin can be located by stream miles measured upstream from the mouth of each stream, by county, by quadrangle, and by latitude and longitude.