Evidence of autumn nitrogen limitation and contribution of picoplankton to carbon fixation in Lake Tahoe

Water samples were collected from Lake Tahoe at midlake and nearshore stations at three depths (25, 50, and 75 m, corresponding to light intensities of approximately 10, 1, and 0.1% of surface light) in May and September. The water samples were evaluated to decide the following: (1) whether carbon fixation was N-limited and whether there were differences between picoplankton and larger phytoplankters and (2) the relative importance of picoplankton carbon fixation and related photosynthetic parameters. Evidence of N-limitation was observed only in September at the midlake station for the populations at 50 and 75 m, and to a lesser extent at the nearshore station at 75 m, but only for the nanoplankton fraction. The contribution of picoplankton to total carbon fixation ranged from 34 to 69% and did not increase with depth. Low values for I_k, the photoadaptation parameter, for deep water populations (0.1% light level) in September, but not May, suggested that these populations were adapted to low light in the fall but not the spring.