During February and March 1964, the U. S. Geological Survey ran caliper, conductance, and temperature logs on several wells in the Sarasota area. The Florida Geological Survey had previously run gamma ray and electric logs on the same wells. Two flowing wells were selected for packer testing. The two wells are about the same depth, penetrate essentially the same geologic horizons, and are about 16 miles apart. The packers were set in the wells between the producing horizons of the formations penetrated and each of the horizons was tested for head, amount of production, and quality of water. One of the tests produced excellent results, indicating that the various producing zones were effectively isolated by the packers and that different quality of water, quantity of water, and a different head was available from each zone. The other test did not show sharp differences but did indicate the extent of contamination of the producing horizons in a flowing well which had been capped for approximately two years. These tests indicate that packer testing can measurably add to knowledge of the separation of permeable zones.