In the 9,950-square mile area of the Suwannee River basin in Florida and Georgia, 17 surface-water stations on 9 streams and several springs were sampled for selected water-quality properties and constituents from August 1968 through December 1977. Analyses from these samples indicate that: (1) the water quality of tributary wetlands controls the water quality of the upper Suwannee River headwaters; (2) groundwater substantially affects the water quality of the Suwannee River basin streams below these headquarters; (3) the water quality of the Suwannee River, and many of its tributaries, is determined by several factors and is not simply related to discharge; and (4) development in the Suwannee River basin has had observable effects on the quality of surface waters.