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In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay

January 1, 1999

A nested PCR was developed for detection of the Clostridium botulinum type C1 toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.

Publication Year 1999
Title In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay
DOI 10.1128/AEM.65.7.3240-3243.1999
Authors Judy L. Williamson, Tonie E. Rocke, Judd M. Aiken
Publication Type Article
Publication Subtype Journal Article
Series Title Applied and Environmental Microbiology
Index ID 70021559
Record Source USGS Publications Warehouse
USGS Organization National Wildlife Health Center