Radioactivity levels in 10 tissues were monitored for 21 days after adult (1-year-old) blue tilapia, Oreochromis aureus, were fed a single meal of a diet containing 30 μg unlabelled methyltestosterone (MT) per gram of feed and radiolabelled MT (3H-labelled steroid nucleus and 14C-labelled 17α-methyl group). Radioactivity was highest in all tissues 6–12 h after the feeding; about 90% of the radioactivity was in the digestive tract, liver, gall bladder, and kidney. Radioactivity declined nearly 90% by 4 days and only 0.5% of original radioactivity (67 ng/g of fish) remained after 21 days. Half of the remaining exogenous hormone was in digestive and excretory tissues; concentrations in muscle were less than 1 ng/g of tissue. Ratios of 3H:14C in tissues were similar to those incorporated in the diet and suggested that the 17α-methyl group was not removed during metabolism.