We documented 17 white sturgeon Acipenser transmontanus spawning locations in the Snake River from the mouth to Lower Granite Dam (river km 0 to 173). Spawning locations were determined by the collection of fertilized eggs on artificial substrates or in plankton nets. We collected 245 eggs at seven locations in McNary Reservoir, 22 eggs at three locations in Ice Harbor Reservoir, 30 eggs from two locations in Lower Monumental Reservoir, and 464 eggs at five locations in Little Goose Reservoir. All 17 locations were in high water velocity areas and between 1.0 and 7.0 km downstream from a hydroelectric dam. The documentation of spawning areas is important because this habitat is necessary to maintain natural and viable populations.