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Explore Search: Quantitative polymerase chain reaction (qPCR)

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Capability
Photo of fluorescence amplification curves from a quantitative polymerase chain reaction (qPCR) analysis

Real-time quantitative polymerase chain reaction (qPCR)

Data Release
Collage of images of an earthquake, scientists in flood with instruments, a volcano erupting, and seismic illustration.

Quantitative polymerase chain reaction (qPCR) cyanotoxin data for 21 sites on Lake Okeechobee and the Okeechobee waterway, Florida, USA collected monthly from March 2019 through October 2021

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Evaluation of Real-Time Quantitative Polymerase Chain Reaction (qPCR) to Determine Escherichia coli Concentrations at Two Lake Erie Beaches

Data Release
Collage of images of an earthquake, scientists in flood with instruments, a volcano erupting, and seismic illustration.

Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan.

Data Release
Collage of images of an earthquake, scientists in flood with instruments, a volcano erupting, and seismic illustration.

Molecular Sexing of Birds Using Quantitative PCR (qPCR) of Sex-Linked Genes and Logistic Models

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The use of epifluorescent microscopy and quantitative polymerase chain reaction to determine the presence/absence and identification of microorganisms associated with domestic and foreign wallboard samples

Data Release
Collage of images of an earthquake, scientists in flood with instruments, a volcano erupting, and seismic illustration.

Quantitative polymerase chain reaction (qPCR) cyanotoxin data for eight mesocosm experiments in the Caloosahatchee River, Florida from May 2019 through August 2021

Data Release
Collage of images of an earthquake, scientists in flood with instruments, a volcano erupting, and seismic illustration.

Quantification of nitrogen-fixing microorganisms (targeting nifH gene) in Cladophora algae by quantitative polymerase chain reaction (qPCR)

publication
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Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis

Data Release
Collage of images of an earthquake, scientists in flood with instruments, a volcano erupting, and seismic illustration.

Digital Polymerase Chain Reaction (dPCR) and Presence/Absence Quantitative Polymerase Chain Reaction (qPCR) Data From the Sediment-Bound Contaminant Resiliency and Response Strategy Pilot Study, Northeastern United States, 2015

Photography
qPCR for the detection of Rs

WFRC qPCR for detection of Renibacterium salmoninarum Image 2

Photography
qPCR for the detection of Rs

WFRC qPCR for detection of Renibacterium salmoninarum Image 1

Photography
Photo of a LIDE scientist preparing a sample for qPCR analysis

Preparing a sample for qPCR analysis

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Interlaboratory comparison of three microbial source tracking quantitative polymerase chain reaction (qPCR) assays from fecal-source and environmental samples

Methods, protocols, and procedures

WFRC BACT7 qPCR for detection of Renibacterium salmoninarum (pdf)

Photography
Photo of fluorescence amplification curves from a quantitative polymerase chain reaction (qPCR) analysis

Fluorescence amplification curves from a qPCR analysis

publication
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Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting

Data Release
Collage of images of an earthquake, scientists in flood with instruments, a volcano erupting, and seismic illustration.

Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023)

Research
Development of methods distinguishing live and dead cell DNA

Development of methods distinguishing live and dead cell DNA for qPCR detection and quantification of pathogen genes in environmental samples for quantitative microbial risk assessment- Carrie Givens

Photography
Filtration system used for processing samples

Filtration system used for processing samples

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Linking non-culturable (qPCR) and culturable enterococci densities with hydrometeorological conditions

Capability
Photo of glass-wool filters used to concentrate waterborne virus material

Sampling and analytical capabilities

Data Release
Collage of images of an earthquake, scientists in flood with instruments, a volcano erupting, and seismic illustration.

Avian Botulism qPCR data, Leelanau Peninsula, Michigan. Years 2011-2014

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Multi-laboratory survey of qPCR enterococci analysis method performance in U.S. coastal and inland surface waters

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Relationship and variation of qPCR and culturable enterococci estimates in ambient surface waters are predictable

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Relations between DNA- and RNA-based molecular methods for cyanobacteria and microcystin concentration at Maumee Bay State Park Lakeside Beach, Oregon, Ohio, 2012

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Development and validation of a quantitative PCR to detect Parvicapsula minibicornis and comparison to histologically ranked infection of juvenile Chinook salmon, Oncorhynchus tshawytscha (Walbaum), from the Klamath River, USA

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Annual variations in microcystin occurrence in Upper Klamath Lake, Oregon, based on high-throughput DNA sequencing, qPCR, and environmental parameters

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Detection of Nanophyetus salmincola in water, snails, and fish tissues by quantitative polymerase chain reaction

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Nitrate reduction mechanisms and rates in an unconfined eogenetic karst aquifer in two sites with different redox potential