Landscape transcriptomic analysis detects thermal stress responses and potential adaptive variation in wild brook trout during successive heatwaves

Extreme weather events, such as heatwaves, are becoming more frequent and intense as a result of climate change. Importantly, such extreme weather events can be more important drivers of extirpation and selection than changes in annual or seasonal averages and they pose a particularly large threat to poikilothermic organisms. In this study, we evaluate the thermal stress response of a coldwater adapted fish species, the eastern brook trout (*Salvelinus fontinalis*), to two successive heatwaves during July and August 2022.