Courtney Grady
(She/Her)Courtney is a laboratory technician at the Marrowstone Marine Field Station.
Courtney provides technical support at the field station in wild fish disease experiments and field surveys.
Professional Experience
2023 - Present: Biological Laboratory Technician, USGS Marrowstone Marine Field Station, Nordland, WA
2020 – 2023: Laboratory Technologist, Labcorp, Overland Park, KS and Phoenix, AZ
2014: Fisheries Biologist 1, Pacific States Marine Fisheries Commission, North Bonneville, WA
2011 – 2013: Rural Aquaculture Promotion Extension Agent, US Peace Corps, Mbwindi, Zambia
2007 – 2011: Biological Laboratory Technician, USGS Marrowstone Marine Field Station, Nordland, WA
2004 – 2007: Research Assistant, Seattle Biomedical Research Institute, Seattle, WA
Education and Certifications
B.S. Biology, Seattle Pacific University, Seattle, WA
Science and Products
Filter Total Items: 14
Susceptibility of Pacific herring to viral hemorrhagic septicemia is influenced by diet
Groups of specific-pathogen-free Pacific herring Clupea pallasii were highly susceptible to infection by viral hemorrhagic septicemia virus (VHSV); however, the level of mortality was influenced by diet during the 40–71 d before, during, and after the first exposure to the virus. Cumulative mortality was highest among the herring maintained on an experimental soy-based pellet, intermediate among t
Authors
Joshua Beaulaurier, N. Bickford, J.L. Gregg, C.A. Grady, A.L. Gannam, J. R. Winton, P.K. Hershberger
Kinetics of viral load and erythrocytic inclusion body formation in pacific herring artificially infected with erythrocytic necrosis virus
Viral erythrocytic necrosis (VEN) is a condition that affects marine and anadromous fish species, including herrings and salmonids, in the Atlantic and Pacific oceans. Infection is frequently associated with severe anemia and causes episodic mortality among wild and hatchery fish when accompanied by additional stressors; VEN can be presumptively diagnosed by (1) light microscopic identification of
Authors
Jolene A. Glenn, Eveline J. Emmenegger, Courtney A. Grady, Sean R. Roon, Jacob L. Gregg, Carla M. Conway, James R. Winton, Paul K. Hershberger
Inability to demonstrate fish-to-fish transmission of Ichthyophonus from laboratory infected Pacific herring Clupea pallasii to naïve conspecifics
The parasite Ichthyophonus is enzootic in many marine fish populations of the northern Atlantic and Pacific Oceans. Forage fishes are a likely source of infection for higher trophic level predators; however, the processes that maintain Ichthyophonus in forage fish populations (primarily clupeids) are not well understood. Lack of an identified intermediate host has led to the convenient hypothesis
Authors
J.L. Gregg, C.A. Grady, C.S. Friedman, P.K. Hershberger
Efficacy of a glycoprotein DNA vaccine against viral haemorrhagic septicaemia (VHS) in Pacific herring, Clupea pallasii Valenciennes
Viral haemorrhagic septicaemia virus (VHSV) and its associated disease state, viral haemorrhagic septicaemia (VHS), is hypothesized to be a proximate factor accounting for the decline and failed recovery of Pacific herring populations in Prince William Sound, AK (Marty et al. 1998, 2003, 2010). Survivors of laboratory-induced VHSV epizootics develop resistance to subsequent viral exposure (Kocan e
Authors
L.M. Hart, Niels Lorenzen, S. E. LaPatra, C.A. Grady, S.E. Roon, J. O’Reilly, J.L. Gregg, P.K. Hershberger
Viral replication in excised fin tissues (VREFT) corresponds with prior exposure of Pacific herring, Clupea pallasii (Valenciennes), to viral haemorrhagic septicaemia virus (VHSV)
Procedures for a viral replication in excised fin tissue (VREFT) assay were adapted to Pacific herring, Clupea pallasii, and optimized both to reduce processing time and to provide the greatest resolution between na??ve herring and those previously exposed to viral haemorrhagic septicaemia virus (VHSV), Genogroup IVa. The optimized procedures included removal of the left pectoral fin from a euthan
Authors
C.A. Grady, J.L. Gregg, R.M. Wade, J. R. Winton, P.K. Hershberger
Effects of environmental temperature on the dynamics of ichthyophoniasis in Juvenile Pacific Herring (Clupea pallasii)
The effects of temperature and infection by Ichthyophonus were examined in juvenile Pacific herring (Clupea pallasii) maintained under simulated overwinter fasting conditions. In addition to defining parameters for a herring bioenergetics model (discussed in Vollenweider et al. this issue), these experiments provided new insights into factors influencing the infectivity and virulence of the parasi
Authors
J.L. Gregg, Johanna J. Vollenweider, C.A. Grady, R.A. Heintz, P.K. Hershberger
Larval and juvenile Pacific herring Clupea pallasii are not susceptible to infectious hematopoietic necrosis under laboratory conditions
Infectious hematopoietic necrosis (IHN) leads to periodic epidemics among certain wild and farmed fish species of the Northeast (NE) Pacific. The source of the IHN virus (IHNV) that initiates these outbreaks remains unknown; however, a leading hypothesis involves viral persistence in marine host species such as Pacific herring Clupea pallasii. Under laboratory conditions we exposed specific patho
Authors
L.M. Hart, G.S. Traxler, K.A. Garver, J. Richard, J.L. Gregg, C.A. Grady, Gael Kurath, P.K. Hershberger
Passive immunization of Pacific herring against viral hemorrhagic septicemia.
The plasma of Pacific herring Clupea pallasii that survived laboratory-induced viral hemorrhagic septicemia (VHS) epizootics contained humoral substances that, when injected into naive animals, conferred passive immunity against the disease. Among groups exposed to viral hemorrhagic septicemia virus (VHSV), injection of donor plasma from VHS survivors resulted in significantly greater survival (50
Authors
P.K. Hershberger, J.L. Gregg, C.A. Grady, S. E. LaPatra, J. R. Winton
Factors controlling the early stages of viral haemorrhagic septicaemia epizootics: Low exposure levels, virus amplification and fish-to-fish transmission
Viral haemorrhagic septicaemia virus, Genogroup IVa (VHSV), was highly infectious to Pacific herring, Clupea pallasii (Valenciennes), even at exposure doses occurring below the threshold of sensitivity for a standard viral plaque assay; however, further progression of the disease to a population‐level epizootic required viral amplification and effective fish‐to‐fish transmission. Among groups of h
Authors
P.K. Hershberger, J.L. Gregg, C.A. Grady, L.M. Hart, S.R. Roon, J. R. Winton
Susceptibility of three stocks of pacific herring to viral hemorrhagic septicemia
Laboratory challenges using specific-pathogen-free Pacific herring Clupea pallasii from three distinct populations indicated that stock origin had no effect on susceptibility to viral hemorrhagic septicemia (VHS). All of the populations were highly susceptible to the disease upon initial exposure, with significantly greater cumulative mortalities occurring in the exposed treatment groups (56.3-64.
Authors
P.K. Hershberger, J.L. Gregg, C.A. Grady, R.M. Collins
Kinetics of viral shedding provide insights into the epidemiology of viral hemorrhagic septicemia in Pacific herring
Losses from infectious diseases are an important component of natural mortality among marine fish species, but factors controlling the ecology of these diseases and their potential responses to anthropogenic changes are poorly understood. We used viral hemorrhagic septicemia virus (VHSV) and a laboratory stock of Pacific herring Clupea pallasii to investigate the kinetics of viral shedding and its
Authors
Paul K. Hershberger, Jacob L. Gregg, James R. Winton, Courtney Grady, Rachael Collins
Chronic and persistent viral hemorrhagic septicemia virus infections in Pacific herring
Chronic viral hemorrhagic septicemia virus (VHSV) infections were established in a laboratory stock of Pacific herring Clupea pallasii held in a large-volume tank supplied with pathogen-free seawater at temperatures ranging from 6.8 to 11.6°C. The infections were characterized by viral persistence for extended periods and near-background levels of host mortality. Infectious virus was recovered fro
Authors
Paul K. Hershberger, Jacob L. Gregg, James R. Winton, Cortney A. Grady, L. Taylor
Non-USGS Publications**
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 14
Susceptibility of Pacific herring to viral hemorrhagic septicemia is influenced by diet
Groups of specific-pathogen-free Pacific herring Clupea pallasii were highly susceptible to infection by viral hemorrhagic septicemia virus (VHSV); however, the level of mortality was influenced by diet during the 40–71 d before, during, and after the first exposure to the virus. Cumulative mortality was highest among the herring maintained on an experimental soy-based pellet, intermediate among t
Authors
Joshua Beaulaurier, N. Bickford, J.L. Gregg, C.A. Grady, A.L. Gannam, J. R. Winton, P.K. Hershberger
Kinetics of viral load and erythrocytic inclusion body formation in pacific herring artificially infected with erythrocytic necrosis virus
Viral erythrocytic necrosis (VEN) is a condition that affects marine and anadromous fish species, including herrings and salmonids, in the Atlantic and Pacific oceans. Infection is frequently associated with severe anemia and causes episodic mortality among wild and hatchery fish when accompanied by additional stressors; VEN can be presumptively diagnosed by (1) light microscopic identification of
Authors
Jolene A. Glenn, Eveline J. Emmenegger, Courtney A. Grady, Sean R. Roon, Jacob L. Gregg, Carla M. Conway, James R. Winton, Paul K. Hershberger
Inability to demonstrate fish-to-fish transmission of Ichthyophonus from laboratory infected Pacific herring Clupea pallasii to naïve conspecifics
The parasite Ichthyophonus is enzootic in many marine fish populations of the northern Atlantic and Pacific Oceans. Forage fishes are a likely source of infection for higher trophic level predators; however, the processes that maintain Ichthyophonus in forage fish populations (primarily clupeids) are not well understood. Lack of an identified intermediate host has led to the convenient hypothesis
Authors
J.L. Gregg, C.A. Grady, C.S. Friedman, P.K. Hershberger
Efficacy of a glycoprotein DNA vaccine against viral haemorrhagic septicaemia (VHS) in Pacific herring, Clupea pallasii Valenciennes
Viral haemorrhagic septicaemia virus (VHSV) and its associated disease state, viral haemorrhagic septicaemia (VHS), is hypothesized to be a proximate factor accounting for the decline and failed recovery of Pacific herring populations in Prince William Sound, AK (Marty et al. 1998, 2003, 2010). Survivors of laboratory-induced VHSV epizootics develop resistance to subsequent viral exposure (Kocan e
Authors
L.M. Hart, Niels Lorenzen, S. E. LaPatra, C.A. Grady, S.E. Roon, J. O’Reilly, J.L. Gregg, P.K. Hershberger
Viral replication in excised fin tissues (VREFT) corresponds with prior exposure of Pacific herring, Clupea pallasii (Valenciennes), to viral haemorrhagic septicaemia virus (VHSV)
Procedures for a viral replication in excised fin tissue (VREFT) assay were adapted to Pacific herring, Clupea pallasii, and optimized both to reduce processing time and to provide the greatest resolution between na??ve herring and those previously exposed to viral haemorrhagic septicaemia virus (VHSV), Genogroup IVa. The optimized procedures included removal of the left pectoral fin from a euthan
Authors
C.A. Grady, J.L. Gregg, R.M. Wade, J. R. Winton, P.K. Hershberger
Effects of environmental temperature on the dynamics of ichthyophoniasis in Juvenile Pacific Herring (Clupea pallasii)
The effects of temperature and infection by Ichthyophonus were examined in juvenile Pacific herring (Clupea pallasii) maintained under simulated overwinter fasting conditions. In addition to defining parameters for a herring bioenergetics model (discussed in Vollenweider et al. this issue), these experiments provided new insights into factors influencing the infectivity and virulence of the parasi
Authors
J.L. Gregg, Johanna J. Vollenweider, C.A. Grady, R.A. Heintz, P.K. Hershberger
Larval and juvenile Pacific herring Clupea pallasii are not susceptible to infectious hematopoietic necrosis under laboratory conditions
Infectious hematopoietic necrosis (IHN) leads to periodic epidemics among certain wild and farmed fish species of the Northeast (NE) Pacific. The source of the IHN virus (IHNV) that initiates these outbreaks remains unknown; however, a leading hypothesis involves viral persistence in marine host species such as Pacific herring Clupea pallasii. Under laboratory conditions we exposed specific patho
Authors
L.M. Hart, G.S. Traxler, K.A. Garver, J. Richard, J.L. Gregg, C.A. Grady, Gael Kurath, P.K. Hershberger
Passive immunization of Pacific herring against viral hemorrhagic septicemia.
The plasma of Pacific herring Clupea pallasii that survived laboratory-induced viral hemorrhagic septicemia (VHS) epizootics contained humoral substances that, when injected into naive animals, conferred passive immunity against the disease. Among groups exposed to viral hemorrhagic septicemia virus (VHSV), injection of donor plasma from VHS survivors resulted in significantly greater survival (50
Authors
P.K. Hershberger, J.L. Gregg, C.A. Grady, S. E. LaPatra, J. R. Winton
Factors controlling the early stages of viral haemorrhagic septicaemia epizootics: Low exposure levels, virus amplification and fish-to-fish transmission
Viral haemorrhagic septicaemia virus, Genogroup IVa (VHSV), was highly infectious to Pacific herring, Clupea pallasii (Valenciennes), even at exposure doses occurring below the threshold of sensitivity for a standard viral plaque assay; however, further progression of the disease to a population‐level epizootic required viral amplification and effective fish‐to‐fish transmission. Among groups of h
Authors
P.K. Hershberger, J.L. Gregg, C.A. Grady, L.M. Hart, S.R. Roon, J. R. Winton
Susceptibility of three stocks of pacific herring to viral hemorrhagic septicemia
Laboratory challenges using specific-pathogen-free Pacific herring Clupea pallasii from three distinct populations indicated that stock origin had no effect on susceptibility to viral hemorrhagic septicemia (VHS). All of the populations were highly susceptible to the disease upon initial exposure, with significantly greater cumulative mortalities occurring in the exposed treatment groups (56.3-64.
Authors
P.K. Hershberger, J.L. Gregg, C.A. Grady, R.M. Collins
Kinetics of viral shedding provide insights into the epidemiology of viral hemorrhagic septicemia in Pacific herring
Losses from infectious diseases are an important component of natural mortality among marine fish species, but factors controlling the ecology of these diseases and their potential responses to anthropogenic changes are poorly understood. We used viral hemorrhagic septicemia virus (VHSV) and a laboratory stock of Pacific herring Clupea pallasii to investigate the kinetics of viral shedding and its
Authors
Paul K. Hershberger, Jacob L. Gregg, James R. Winton, Courtney Grady, Rachael Collins
Chronic and persistent viral hemorrhagic septicemia virus infections in Pacific herring
Chronic viral hemorrhagic septicemia virus (VHSV) infections were established in a laboratory stock of Pacific herring Clupea pallasii held in a large-volume tank supplied with pathogen-free seawater at temperatures ranging from 6.8 to 11.6°C. The infections were characterized by viral persistence for extended periods and near-background levels of host mortality. Infectious virus was recovered fro
Authors
Paul K. Hershberger, Jacob L. Gregg, James R. Winton, Cortney A. Grady, L. Taylor
Non-USGS Publications**
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.