Dale Griffin, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 87
Terrestrial microorganisms at an altitude of 20,000 m in Earth's atmosphere
A joint effort between the U.S. Geological Survey's (USGS) Global Desert Dust and NASA's Stratospheric and Cosmic Dust Programs identified culturable microbes from an air sample collected at an altitude of 20,000 m. A total of 4 fungal (Penicillium sp.) and 71 bacteria colonyforming units (70 colonies of Bacillus luciferensis believed to have originated from a single cell collected at altitude and
Authors
Dale W. Griffin
Pathogenic human viruses in coastal waters
This review addresses both historical and recent investigations into viral contamination of marine waters. With the relatively recent emergence of molecular biology-based assays, a number of investigations have shown that pathogenic viruses are prevalent in marine waters being impacted by sewage. Research has shown that this group of fecal-oral viral pathogens (enteroviruses, hepatitis A viruses,
Authors
Dale W. Griffin, Kim A. Donaldson, J.H. Paul, Joan B. Rose
Supraglacial sulfur springs and associated biological activity in the Canadian high arctic - signs of life beneath the ice
Unique springs, discharging from the surface of an arctic glacier, release H2S and deposit native sulfur, gypsum, and calcite. The presence of sulfur in three oxidation states indicates a complex series of redox reactions. Physical and chemical conditions of the spring water and surrounding environment, as well as mineralogical and isotopic signatures, suggest biologically mediated reactions. Cell
Authors
Stephen E. Grasby, Carlton C. Allen, Teresa G. Longazo, John T. Lisle, Dale W. Griffin, Benoit Beauchamp
African and Asian dust: from desert soils to coral reefs
Many hypotheses have been proposed to explain the decline of coral reefs throughout the world, but none adequately accounts for the lack of recovery of reefs or the wide geographical distribution of coral diseases. The processes driving the decline remain elusive. Hundreds of millions of tons of dust transported annually from Africa and Asia to the Americas may be adversely affecting coral reefs a
Authors
Virginia H. Garrison, Eugene A. Shinn, William T. Foreman, Dale W. Griffin, Charles W. Holmes, Christina A. Kellogg, Michael S. Majewski, Laurie L. Richardson, Kim B. Ritchie, Garriet W. Smith
Atmospheric transport of mold spores in clouds of desert dust
Fungal spores can be transported globally in clouds of desert dust. Many species of fungi (commonly known as molds) and bacteria--including some that are human pathogens--have characteristics suited to long-range atmospheric transport. Dust from the African desert can affect air quality in Africa, Europe, the Middle East, and the Americas. Asian desert dust can affect air quality in Asia, the Arct
Authors
E.A. Shinn, Dale W. Griffin, D.B. Seba
African dust carries microbes across the ocean: are they affecting human and ecosystem health?
Atmospheric transport of dust from northwest Africa to the western Atlantic Ocean region may be responsible for a number of environmental hazards, including the demise of Caribbean corals; red tides; amphibian diseases; increased occurrence of asthma in humans; and oxygen depletion (eutrophication) in estuaries. Studies of satellite images suggest that hundreds of millions of tons of dust are tran
Authors
Christina A. Kellogg, Dale W. Griffin
Florida springs interdisciplinary science study
No abstract available.
Authors
Brian G. Katz, Dale W. Griffin, Peter W. Swarzenski, Stephen J. Walsh, Howard L. Jelks
Atmospheric microbiology in the northern Caribbean during African dust events
Between July 2000 and August 2001 forty-three air samples were collected in the northern Caribbean: Twenty-six in the US Virgin Islands, and 17 samples aboard ship during two 1-week cruises. Samples were collected during African dust events and non-dust conditions and screened for the presence of culturable bacteria and fungi. A total of 3,652 liters of air were collected during non-dust condition
Authors
Dale W. Griffin, C.A. Kellogg, V.H. Garrison, J.T. Lisle, T.C. Borden, E.A. Shinn
Biogeochemical sulphur cycle in an extreme environment - Life beneath a high arctic glacier, Nunavut, Canada
Unique springs discharge from the surface of a high arctic glacier, releasing H2S, and depositing native sulphur, gypsum, and calcite. A rare CaCO3 polymorph, vaterite, is also observed. Physical and chemical conditions of the spring water and surrounding environment, as well as mineralogical and isotopic signatures, argue for biologically mediated redox reactions controlling sulfur. Cell counts a
Authors
S.E. Grasby, C. C. Allen, T.G. Longazo, J.T. Lisle, Dale W. Griffin, B. Beauchamp
The movement of soil and sediment in Earth's atmosphere: microbiology and ecosystem health
No abstract available.
Authors
Dale W. Griffin, C.A. Kellogg, V.H. Garrison, C. Holmes, E.A. Shinn
Detection, quantitation and identification of enteroviruses from surface waters and sponge tissue from the Florida Keys using real-time RT-PCR
A method was developed for the quantitative detection of pathogenic human enteroviruses from surface waters in the Florida Keys using Taqman (R) one-step Reverse transcription (RT)-PCR with the Model 7700 ABI Prism (R) Sequence Detection System. Viruses were directly extracted from unconcentrated grab samples of seawater, from seawater concentrated by vortex flow filtration using a 100kD filter an
Authors
K.A. Donaldson, Dale W. Griffin, J.H. Paul
A rapid and efficient assay for extracting DNA from fungi
Aims: A method for the rapid extraction of fungal DNA from small quantities of tissue in a batch-processing format was investigated. Methods and Results: Tissue (< 3.0 mg) was scraped from freshly-grown fungal isolates. The tissue was suspended in buffer AP1 and subjected to seven rounds of freeze/thaw using a crushed dry ice/ethanol bath and a boiling water bath. After a 30 min boiling step, the
Authors
Dale W. Griffin, C.A. Kellogg, K.K. Peak, E.A. Shinn
Science and Products
Filter Total Items: 87
Terrestrial microorganisms at an altitude of 20,000 m in Earth's atmosphere
A joint effort between the U.S. Geological Survey's (USGS) Global Desert Dust and NASA's Stratospheric and Cosmic Dust Programs identified culturable microbes from an air sample collected at an altitude of 20,000 m. A total of 4 fungal (Penicillium sp.) and 71 bacteria colonyforming units (70 colonies of Bacillus luciferensis believed to have originated from a single cell collected at altitude and
Authors
Dale W. Griffin
Pathogenic human viruses in coastal waters
This review addresses both historical and recent investigations into viral contamination of marine waters. With the relatively recent emergence of molecular biology-based assays, a number of investigations have shown that pathogenic viruses are prevalent in marine waters being impacted by sewage. Research has shown that this group of fecal-oral viral pathogens (enteroviruses, hepatitis A viruses,
Authors
Dale W. Griffin, Kim A. Donaldson, J.H. Paul, Joan B. Rose
Supraglacial sulfur springs and associated biological activity in the Canadian high arctic - signs of life beneath the ice
Unique springs, discharging from the surface of an arctic glacier, release H2S and deposit native sulfur, gypsum, and calcite. The presence of sulfur in three oxidation states indicates a complex series of redox reactions. Physical and chemical conditions of the spring water and surrounding environment, as well as mineralogical and isotopic signatures, suggest biologically mediated reactions. Cell
Authors
Stephen E. Grasby, Carlton C. Allen, Teresa G. Longazo, John T. Lisle, Dale W. Griffin, Benoit Beauchamp
African and Asian dust: from desert soils to coral reefs
Many hypotheses have been proposed to explain the decline of coral reefs throughout the world, but none adequately accounts for the lack of recovery of reefs or the wide geographical distribution of coral diseases. The processes driving the decline remain elusive. Hundreds of millions of tons of dust transported annually from Africa and Asia to the Americas may be adversely affecting coral reefs a
Authors
Virginia H. Garrison, Eugene A. Shinn, William T. Foreman, Dale W. Griffin, Charles W. Holmes, Christina A. Kellogg, Michael S. Majewski, Laurie L. Richardson, Kim B. Ritchie, Garriet W. Smith
Atmospheric transport of mold spores in clouds of desert dust
Fungal spores can be transported globally in clouds of desert dust. Many species of fungi (commonly known as molds) and bacteria--including some that are human pathogens--have characteristics suited to long-range atmospheric transport. Dust from the African desert can affect air quality in Africa, Europe, the Middle East, and the Americas. Asian desert dust can affect air quality in Asia, the Arct
Authors
E.A. Shinn, Dale W. Griffin, D.B. Seba
African dust carries microbes across the ocean: are they affecting human and ecosystem health?
Atmospheric transport of dust from northwest Africa to the western Atlantic Ocean region may be responsible for a number of environmental hazards, including the demise of Caribbean corals; red tides; amphibian diseases; increased occurrence of asthma in humans; and oxygen depletion (eutrophication) in estuaries. Studies of satellite images suggest that hundreds of millions of tons of dust are tran
Authors
Christina A. Kellogg, Dale W. Griffin
Florida springs interdisciplinary science study
No abstract available.
Authors
Brian G. Katz, Dale W. Griffin, Peter W. Swarzenski, Stephen J. Walsh, Howard L. Jelks
Atmospheric microbiology in the northern Caribbean during African dust events
Between July 2000 and August 2001 forty-three air samples were collected in the northern Caribbean: Twenty-six in the US Virgin Islands, and 17 samples aboard ship during two 1-week cruises. Samples were collected during African dust events and non-dust conditions and screened for the presence of culturable bacteria and fungi. A total of 3,652 liters of air were collected during non-dust condition
Authors
Dale W. Griffin, C.A. Kellogg, V.H. Garrison, J.T. Lisle, T.C. Borden, E.A. Shinn
Biogeochemical sulphur cycle in an extreme environment - Life beneath a high arctic glacier, Nunavut, Canada
Unique springs discharge from the surface of a high arctic glacier, releasing H2S, and depositing native sulphur, gypsum, and calcite. A rare CaCO3 polymorph, vaterite, is also observed. Physical and chemical conditions of the spring water and surrounding environment, as well as mineralogical and isotopic signatures, argue for biologically mediated redox reactions controlling sulfur. Cell counts a
Authors
S.E. Grasby, C. C. Allen, T.G. Longazo, J.T. Lisle, Dale W. Griffin, B. Beauchamp
The movement of soil and sediment in Earth's atmosphere: microbiology and ecosystem health
No abstract available.
Authors
Dale W. Griffin, C.A. Kellogg, V.H. Garrison, C. Holmes, E.A. Shinn
Detection, quantitation and identification of enteroviruses from surface waters and sponge tissue from the Florida Keys using real-time RT-PCR
A method was developed for the quantitative detection of pathogenic human enteroviruses from surface waters in the Florida Keys using Taqman (R) one-step Reverse transcription (RT)-PCR with the Model 7700 ABI Prism (R) Sequence Detection System. Viruses were directly extracted from unconcentrated grab samples of seawater, from seawater concentrated by vortex flow filtration using a 100kD filter an
Authors
K.A. Donaldson, Dale W. Griffin, J.H. Paul
A rapid and efficient assay for extracting DNA from fungi
Aims: A method for the rapid extraction of fungal DNA from small quantities of tissue in a batch-processing format was investigated. Methods and Results: Tissue (< 3.0 mg) was scraped from freshly-grown fungal isolates. The tissue was suspended in buffer AP1 and subjected to seven rounds of freeze/thaw using a crushed dry ice/ethanol bath and a boiling water bath. After a 30 min boiling step, the
Authors
Dale W. Griffin, C.A. Kellogg, K.K. Peak, E.A. Shinn