Jason Ferrante , Ph.D. (Former Employee)
Science and Products
Filter Total Items: 14
Development and validation of quantitative PCR assays to measure cytokine transcript levels in the Florida manatee (Trichechus manatus latirostris)
Cytokines have important roles in the mammalian response to viral and bacterial infections, trauma, and wound healing. Because of early cytokine production after physiologic stresses, the regulation of messenger RNA (mRNA) transcripts can be used to assess immunologic responses before changes in protein production. To detect and assess early immune changes in endangered Florida manatees (Trichechu
Authors
Jason A. Ferrante, Margaret Hunter, James F.X. Wellehan
Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA
A set of universal guidelines is needed to determine the limit of detection (LOD) in PCR-based analyses of low concentration DNA. In particular, environmental DNA (eDNA) studies require sensitive and reliable methods to detect rare and cryptic species through shed genetic material in environmental samples. Current strategies for assessing detection limits of eDNA are either too stringent or subjec
Authors
Margaret Hunter, Robert M. Dorazio, John S. Butterfield, Gaia Meigs-Friend, Leo Nico, Jason A. Ferrante
Science and Products
Filter Total Items: 14
Development and validation of quantitative PCR assays to measure cytokine transcript levels in the Florida manatee (Trichechus manatus latirostris)
Cytokines have important roles in the mammalian response to viral and bacterial infections, trauma, and wound healing. Because of early cytokine production after physiologic stresses, the regulation of messenger RNA (mRNA) transcripts can be used to assess immunologic responses before changes in protein production. To detect and assess early immune changes in endangered Florida manatees (Trichechu
Authors
Jason A. Ferrante, Margaret Hunter, James F.X. Wellehan
Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA
A set of universal guidelines is needed to determine the limit of detection (LOD) in PCR-based analyses of low concentration DNA. In particular, environmental DNA (eDNA) studies require sensitive and reliable methods to detect rare and cryptic species through shed genetic material in environmental samples. Current strategies for assessing detection limits of eDNA are either too stringent or subjec
Authors
Margaret Hunter, Robert M. Dorazio, John S. Butterfield, Gaia Meigs-Friend, Leo Nico, Jason A. Ferrante