Ronald Oremland (Former Employee)
Science and Products
Enhancement of Trichloroethene (TCE) Biodegradation in a Simulated Groundwater System
This laboratory-based study provides information for understanding enhancement of trichloroethene (TCE) biodegradation in a simulated groundwater system.
Acetylene Consumption and Dechlorination by a Groundwater Microbial Enrichment Culture
This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Tren
Growth of cultured Picocystis strain ML in the presence of arsenic, and occurrence of arsenolipids in these Picocystis as well as biota and sediment from Mono Lake, California
Mono Lake is a hypersaline soda lake rich in dissolved inorganic arsenic with its primary production currently dominated by Picocystis str. ML. We set out to determine if this picoplankter could metabolize inorganic arsenic, and in doing so form unusual arsenolipids (e.g., methylated arsinoyl ribosides) as reported in other saline ecosystems and by halophilic algae. We cultivated Picocystis str. M
Filter Total Items: 119
Acetylenotrophic and diazotrophic Bradyrhizobium sp. strain I71 from TCE-contaminated soils
AbstractAcetylene (C2H2) is a molecule rarely found in nature, with very few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs; however, we hypothesize there may yet be unrecognized diversity of acetylenotrophs in nature. This study expands the known
Authors
Denise M. Akob, John M. Sutton, Timothy J. Bushman, Shaun Baesman, Edina Klein, Yesha Shrestha, Robert Andrews, Janna L. Fierst, Max Kolton, Sara Gushgari-Doyle, Ronald Oremland, John Freeman
Got acetylene: A personal research retrospective
In research, sometimes sheer happenstance and serendipity make for an unexpected discovery. Once revealed and if interesting enough, such a finding and its follow-up investigations can lead to advances by others that leave its originators ‘scooped’ and mulling about what next to do with their unpublished data, specifically what journals could it still be published in and be perceived as original.
Authors
Ronald S. Oremland
Syntrophotalea acetylenivorans sp. nov., a diazotrophic, acetylenotrophic anaerobe isolated from intertidal sediments
A Gram-stain-negative, strictly anaerobic, non-motile, rod-shaped bacterium, designated SFB93T, was isolated from the intertidal sediments of South San Francisco Bay, located near Palo Alto, CA, USA. SFB93T was capable of acetylenotrophic and diazotrophic growth, grew at 22–37 °C, pH 6.3–8.5 and in the presence of 10–45 g l−1 NaCl. Phylogenetic analyses based on 16S rRNA gene sequencing showed tha
Authors
Shaun Baesman, John M. Sutton, Janna L. Fierst, Denise M. Akob, Ronald S. Oremland
Acetylene-fueled trichloroethene reductive dechlorination in a groundwater enrichment culture
In aquifers, acetylene (C2H2) is a product of abiotic degradation of trichloroethene (TCE) catalyzed by in situ minerals. C2H2 can, in turn, inhibit multiple microbial processes including TCE dechlorination and metabolisms that commonly support dechlorination, in addition to supporting the growth of acetylenotrophic microorganisms. Previously, C2H2 was shown to support TCE reductive dechlorination
Authors
Sara Gushgari-Doyle, Ronald S. Oremland, Ray Keren, Shaun Baesman, Denise M. Akob, Jillian F. Banfield, Lisa Alvarez-Cohen
Arsenolipids in cultured Picocystis strain ML, and their occurrence in biota and sediment from Mono Lake, California
Primary production in Mono Lake, a hypersaline soda lake rich in dissolved inorganic arsenic, is dominated by Picocystis strain ML. We set out to determine if this photoautotrophic picoplankter could metabolize inorganic arsenic and in doing so form unusual arsenolipids (e.g., arsenic bound to 2-O-methyl ribosides) as reported in other saline ecosystems and by halophilic algae. We cultivated Picoc
Authors
Ronald A. Glabonjat, Jodi S. Blum, Laurence G. Miller, Samuel M. Webb, John F. Stolz, Kevin A. Francesconi, Ronald S. Oremland
Halarsenatibacter
No abstract available.
Authors
Ronald S. Oremland, Jodi S. Blum, John F. Stolz, Chad W Saltikov, Brian Lanoil
Respiratory selenite reductase from Bacillus selenitireducens strain MLS10
The putative respiratory selenite [Se(IV)] reductase (Srr) from Bacillus selenitireducens MLS10 has been identified through a polyphasic approach involving genomics, proteomics, and enzymology. Nondenaturing gel assays were used to identify Srr in cell fractions, and the active band was shown to contain a single protein of 80 kDa. The protein was identified through liquid chromatography-tandem mas
Authors
Michael L. Wells, Jennifer McGarry, Maissa M Gaye, Partha Basu, Ronald S. Oremland, John F. Stolz
Draft genome sequence of Picocystis strain ML cultivated from Mono Lake, California
The microscopic alga Picocystis sp. strain ML is responsible for recurrent algal blooms in Mono Lake, CA. This organism was characterized by only very little molecular data, despite its prominence as a primary producer in saline environments. Here, we report the draft genome sequence for Picocystis sp. strain ML based on long-read sequencing.
Authors
Emily N Junkins, Blake W. Stamps, Frank A Corsetti, Ronald S. Oremland, John R. Spear, Bradley S. Stevenson
Acetylenotrophy: A hidden but ubiquitous microbial metabolism?
Acetylene (IUPAC name: ethyne) is a colorless, gaseous hydrocarbon, composed of two triple bonded carbon atoms attached to hydrogens (C2H2). When microbiologists and biogeochemists think of acetylene, they immediately think of its use as an inhibitory compound of certain microbial processes and a tracer for nitrogen fixation. However, what is less widely known is that anaerobic and aerobic microor
Authors
Denise M. Akob, John M. Sutton, Janna L. Fierst, Karl B. Haase, Shaun Baesman, George Luther, Laurence G. Miller, Ronald S. Oremland
Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93
Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting a mixture of terrestrial and marine emissions from anthropogenic, biomass-burning, and unidentified biogenic sources. Fermentation of acetylene was serendipitously discovered during C2H2 block assays of N2O reductase, and Pelobacter acetylenicus was shown to grow on C2H2 via acetylene hydratase (AH). AH
Authors
Denise M. Akob, Shaun Baesman, John M. Sutton, Janna L. Fierst, Adam Mumford, Yesha Shrestha, Amisha T. Poret-Peterson, Stacy C. Bennett, Darren S. Dunlap, Karl B. Haase, Ronald S. Oremland
Autotrophic microbial arsenotrophy in arsenic-rich soda lakes
A number of prokaryotes are capable of employing arsenic oxy-anions as either electron acceptors [arsenate; As(V)] or electron donors [arsenite; As(III)] to sustain arsenic-dependent growth (‘arsenotrophy’). A subset of these microorganisms function as either chemoautotrophs or photoautotrophs, whereby they gain sufficient energy from their redox metabolism of arsenic to completely satisfy their c
Authors
Ronald S. Oremland, Chad W. Saltikov, John F. Stolz, James T. Hollibaugh
Bacteria versus selenium: A view from the inside out
Bacteria and selenium (Se) are closely interlinked as the element serves both essential nutrient requirements and energy generation functions. However, Se can also behave as a powerful toxicant for bacterial homeostasis. Conversely, bacteria play a tremendous role in the cycling of Se between different environmental compartments, and bacterial metabolism has been shown to participate to all valenc
Authors
Lucian Staicu, Ronald S. Oremland, Ryuta Tobe, Hisaaki Mihara
Science and Products
Enhancement of Trichloroethene (TCE) Biodegradation in a Simulated Groundwater System
This laboratory-based study provides information for understanding enhancement of trichloroethene (TCE) biodegradation in a simulated groundwater system.
Acetylene Consumption and Dechlorination by a Groundwater Microbial Enrichment Culture
This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Tren
Growth of cultured Picocystis strain ML in the presence of arsenic, and occurrence of arsenolipids in these Picocystis as well as biota and sediment from Mono Lake, California
Mono Lake is a hypersaline soda lake rich in dissolved inorganic arsenic with its primary production currently dominated by Picocystis str. ML. We set out to determine if this picoplankter could metabolize inorganic arsenic, and in doing so form unusual arsenolipids (e.g., methylated arsinoyl ribosides) as reported in other saline ecosystems and by halophilic algae. We cultivated Picocystis str. M
Filter Total Items: 119
Acetylenotrophic and diazotrophic Bradyrhizobium sp. strain I71 from TCE-contaminated soils
AbstractAcetylene (C2H2) is a molecule rarely found in nature, with very few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs; however, we hypothesize there may yet be unrecognized diversity of acetylenotrophs in nature. This study expands the known
Authors
Denise M. Akob, John M. Sutton, Timothy J. Bushman, Shaun Baesman, Edina Klein, Yesha Shrestha, Robert Andrews, Janna L. Fierst, Max Kolton, Sara Gushgari-Doyle, Ronald Oremland, John Freeman
Got acetylene: A personal research retrospective
In research, sometimes sheer happenstance and serendipity make for an unexpected discovery. Once revealed and if interesting enough, such a finding and its follow-up investigations can lead to advances by others that leave its originators ‘scooped’ and mulling about what next to do with their unpublished data, specifically what journals could it still be published in and be perceived as original.
Authors
Ronald S. Oremland
Syntrophotalea acetylenivorans sp. nov., a diazotrophic, acetylenotrophic anaerobe isolated from intertidal sediments
A Gram-stain-negative, strictly anaerobic, non-motile, rod-shaped bacterium, designated SFB93T, was isolated from the intertidal sediments of South San Francisco Bay, located near Palo Alto, CA, USA. SFB93T was capable of acetylenotrophic and diazotrophic growth, grew at 22–37 °C, pH 6.3–8.5 and in the presence of 10–45 g l−1 NaCl. Phylogenetic analyses based on 16S rRNA gene sequencing showed tha
Authors
Shaun Baesman, John M. Sutton, Janna L. Fierst, Denise M. Akob, Ronald S. Oremland
Acetylene-fueled trichloroethene reductive dechlorination in a groundwater enrichment culture
In aquifers, acetylene (C2H2) is a product of abiotic degradation of trichloroethene (TCE) catalyzed by in situ minerals. C2H2 can, in turn, inhibit multiple microbial processes including TCE dechlorination and metabolisms that commonly support dechlorination, in addition to supporting the growth of acetylenotrophic microorganisms. Previously, C2H2 was shown to support TCE reductive dechlorination
Authors
Sara Gushgari-Doyle, Ronald S. Oremland, Ray Keren, Shaun Baesman, Denise M. Akob, Jillian F. Banfield, Lisa Alvarez-Cohen
Arsenolipids in cultured Picocystis strain ML, and their occurrence in biota and sediment from Mono Lake, California
Primary production in Mono Lake, a hypersaline soda lake rich in dissolved inorganic arsenic, is dominated by Picocystis strain ML. We set out to determine if this photoautotrophic picoplankter could metabolize inorganic arsenic and in doing so form unusual arsenolipids (e.g., arsenic bound to 2-O-methyl ribosides) as reported in other saline ecosystems and by halophilic algae. We cultivated Picoc
Authors
Ronald A. Glabonjat, Jodi S. Blum, Laurence G. Miller, Samuel M. Webb, John F. Stolz, Kevin A. Francesconi, Ronald S. Oremland
Halarsenatibacter
No abstract available.
Authors
Ronald S. Oremland, Jodi S. Blum, John F. Stolz, Chad W Saltikov, Brian Lanoil
Respiratory selenite reductase from Bacillus selenitireducens strain MLS10
The putative respiratory selenite [Se(IV)] reductase (Srr) from Bacillus selenitireducens MLS10 has been identified through a polyphasic approach involving genomics, proteomics, and enzymology. Nondenaturing gel assays were used to identify Srr in cell fractions, and the active band was shown to contain a single protein of 80 kDa. The protein was identified through liquid chromatography-tandem mas
Authors
Michael L. Wells, Jennifer McGarry, Maissa M Gaye, Partha Basu, Ronald S. Oremland, John F. Stolz
Draft genome sequence of Picocystis strain ML cultivated from Mono Lake, California
The microscopic alga Picocystis sp. strain ML is responsible for recurrent algal blooms in Mono Lake, CA. This organism was characterized by only very little molecular data, despite its prominence as a primary producer in saline environments. Here, we report the draft genome sequence for Picocystis sp. strain ML based on long-read sequencing.
Authors
Emily N Junkins, Blake W. Stamps, Frank A Corsetti, Ronald S. Oremland, John R. Spear, Bradley S. Stevenson
Acetylenotrophy: A hidden but ubiquitous microbial metabolism?
Acetylene (IUPAC name: ethyne) is a colorless, gaseous hydrocarbon, composed of two triple bonded carbon atoms attached to hydrogens (C2H2). When microbiologists and biogeochemists think of acetylene, they immediately think of its use as an inhibitory compound of certain microbial processes and a tracer for nitrogen fixation. However, what is less widely known is that anaerobic and aerobic microor
Authors
Denise M. Akob, John M. Sutton, Janna L. Fierst, Karl B. Haase, Shaun Baesman, George Luther, Laurence G. Miller, Ronald S. Oremland
Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93
Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting a mixture of terrestrial and marine emissions from anthropogenic, biomass-burning, and unidentified biogenic sources. Fermentation of acetylene was serendipitously discovered during C2H2 block assays of N2O reductase, and Pelobacter acetylenicus was shown to grow on C2H2 via acetylene hydratase (AH). AH
Authors
Denise M. Akob, Shaun Baesman, John M. Sutton, Janna L. Fierst, Adam Mumford, Yesha Shrestha, Amisha T. Poret-Peterson, Stacy C. Bennett, Darren S. Dunlap, Karl B. Haase, Ronald S. Oremland
Autotrophic microbial arsenotrophy in arsenic-rich soda lakes
A number of prokaryotes are capable of employing arsenic oxy-anions as either electron acceptors [arsenate; As(V)] or electron donors [arsenite; As(III)] to sustain arsenic-dependent growth (‘arsenotrophy’). A subset of these microorganisms function as either chemoautotrophs or photoautotrophs, whereby they gain sufficient energy from their redox metabolism of arsenic to completely satisfy their c
Authors
Ronald S. Oremland, Chad W. Saltikov, John F. Stolz, James T. Hollibaugh
Bacteria versus selenium: A view from the inside out
Bacteria and selenium (Se) are closely interlinked as the element serves both essential nutrient requirements and energy generation functions. However, Se can also behave as a powerful toxicant for bacterial homeostasis. Conversely, bacteria play a tremendous role in the cycling of Se between different environmental compartments, and bacterial metabolism has been shown to participate to all valenc
Authors
Lucian Staicu, Ronald S. Oremland, Ryuta Tobe, Hisaaki Mihara