Tonie Rocke
Tonie Rocke is a Research Epidemiologist at the National Wildlife Health Center.
Science and Products
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Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction
We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia (Oreochromis mossambicus). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C1 toxin gene. We consistently detected C.
Authors
P. Nol, J.L. Williamson, T.E. Rocke, Thomas M. Yuill
Type C botulism in pelicans and other fish-eating birds at the Salton Sea
In 1996, type C avian botulism killed over 10,000 pelicans and nearly 10,000 other fish-eating birds at the Salton Sea in southern California. Although botulism had been previously documented in waterbirds at the Sea, this die-off was unusual in that it involved primarily fish-eating birds. The American White Pelican (Pelecanus erythrorynchos) was the species with the greatest mortality in 1996. S
Authors
Tonie E. Rocke, Pauline Nol, C. Pelizza, K. K. Sturm
Investigations of a large scale eared grebe (Podiceps nigricollis) die-off at the Salton Sea, California in 1992
An estimated 150,000 Eared Grebes (Podiceps nigricollis) died at the Salton Sea between 16 December 1991 and 21 April 1992. This represented the largest documented mortality event of Eared Grebes at the time and approximately 6% of the North American population. During the die-off, grebes exhibited several uncharacteristic behaviors, such as congregating at freshwater tributaries, repeatedly gulpi
Authors
C.U. Meteyer, D. J. Audet, T.E. Rocke, W. Radke, L. H. Creekmore, R. Duncan
Protection of black-tailed prairie dogs (Cynomys ludovicianus) against plague after voluntary consumption of baits containing recombinant raccoon poxvirus vaccine
Prairie dogs (Cynomys spp.) are highly susceptible to Yersinia pestis and significant reservoirs of plague for humans in the western United States. A recombinant raccoon poxvirus, expressing the F1 antigen of Y. pestis, was incorporated into a palatable bait and offered to 18 black-tailed prairie dogs (Cynomys ludovicianus) for voluntary consumption; 18 negative control animals received placebo ba
Authors
J.S. Mencher, S.R. Smith, T.D. Powell, D.T. Stinchcomb, J.E. Osorio, T.E. Rocke
Could blackbird mortality from avicide DRC-1339 contribute to avian botulism outbreaks in North Dakota?
Blackbird (family lcteridae) depredation on sunflower (Helianthus annuus) crops in the prairie states of the United States has motivated the proposed use of an avicide, DRC-1339 (3-chloro-4-methylaniline), to decrease their numbers. The resulting mortality of blackbirds at wetland roosts could increase the potential of avian botulism occurring in affected marshes. To assess this possibility, we se
Authors
Diana R. Goldberg, M. D. Samuel, T.E. Rocke, K. M. Johnson, G. Linz
Prevalence of neurotoxic Clostridium botulinum type C in the gastrointestinal tracts of tilapis (Oreochromis mossambicus) in the Salton Sea
Tilapia (Oreochromis mossambicus) have been implicated as the source of type C toxin in avian botulism outbreaks in pelicans (Pelecanus erythrorhynchos, Pelecanus occidentalis californicus) at the Salton Sea in southern California (USA). We collected sick, dead, and healthy fish from various sites throughout the Sea during the summers of 1999 through 2001 and tested them for the presence of Clostr
Authors
P.J. Nol, T.E. Rocke, K. Gross, Thomas M. Yuill
Limited infection upon human exposure to a recombinant raccoon pox vaccine vector
A laboratory accident resulted in human exposure to a recombinant raccoon poxvirus (RCN) developed as a vaccine vector for antigens of Yersinia pestis for protection of wild rodents (and other animals) against plague. Within 9 days, the patient developed a small blister that healed within 4 weeks. Raccoon poxvirus was cultured from the lesion, and the patient developed antibody to plague antigen (
Authors
T.E. Rocke, F. J. Dein, M. Fuchsberger, B.C. Fox, D.T. Stinchcomb, J.G. Osorio
Recombinant F1-V fusion protein protects black-footed ferrets (Mustela nigripes) against virulent Yersinia pestis infection
Black-footed ferrets (Mustela nigripes) are highly susceptible to sylvatic plague, caused by the bacterium Yersinia pestis, and this disease has severely hampered efforts to restore ferrets to their historic range. A study was conducted to assess the efficacy of vaccination of black-footed ferrets against plague using a recombinant protein vaccine, designated F1-V, developed by personnel at the U.
Authors
Tonie E. Rocke, J. Mencher, Susan Smith, A. M. Friedlander, G.P. Andrews, L. A. Baeten
Recombinant raccoon pox vaccine protects mice against lethal plague
Using a raccoon poxvirus (RCN) expression system, we have developed new recombinant vaccines that can protect mice against lethal plague infection. We tested the effects of a translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen in tissue culture and the induction of anti
Authors
J.E. Osorio, T.D. Powell, R.S. Frank, K. Moss, E.J. Haanes, S.R. Smith, T.E. Rocke, D.T. Stinchcomb
Determination of the median toxic dose of type C botulism in lactating dairy cows
Because of the difficulty in identifying botulinum toxin in cattle, it is hypothesized that cattle are sensitive to levels of toxin below the detection limits of current diagnostic techniques (the mouse protection bioassay and the immunostick enzyme-linked immunosorbent assay [ELISA] for type C botulinum toxin). Using an up-down method for toxicologic testing, the median toxic dose (MTD50) for cat
Authors
R.B. Moeller, B. Puschner, R.L. Walker, Tonie E. Rocke, F.D. Galey, J.S. Cullor, A.A. Ardans
New host record of avian tuberculosis in an American white pelican, Pelecanus erythrorhynchos
An American white pelican (P. erythrorhyncos) was captured in August 1999 in California, USA. The bird was unable to fly away or evade the boat, and showed considerable aggression when captured. Numerous multifocal, 1 mm diameter, yellow masses were observed throughout the lungs and air sacs. The liver was yellow. A 6 cm diameter, firm spherical mass was present in the abdominal cavity, distal to
Authors
P. Nol, R.E. Brannian, B.M. Berlowski, M. J. Wolcott, T.E. Rocke
Failure to transmit avian vacuolar myelinopathy to mallard ducks
Avian vacuolar myelinopathy (AVM) is a neurologic disease that has been diagnosed in free-ranging birds in the southeastern United States. Bald eagles (Haliaeetus leuocephalus), American coots (Fulica americana), and mallards (Anas platyrhynchos) have been affected. Previous investigations have not determined the etiology of this disease. In November and December 2002, we attempted to induce AVM i
Authors
R. S. Larsen, Felicia B. Nutter, Tom Augspurger, Tonie E. Rocke, Nancy J. Thomas, Michael K. Stoskopf
Science and Products
Filter Total Items: 14
No Result Found
Filter Total Items: 129
Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction
We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia (Oreochromis mossambicus). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C1 toxin gene. We consistently detected C.
Authors
P. Nol, J.L. Williamson, T.E. Rocke, Thomas M. Yuill
Type C botulism in pelicans and other fish-eating birds at the Salton Sea
In 1996, type C avian botulism killed over 10,000 pelicans and nearly 10,000 other fish-eating birds at the Salton Sea in southern California. Although botulism had been previously documented in waterbirds at the Sea, this die-off was unusual in that it involved primarily fish-eating birds. The American White Pelican (Pelecanus erythrorynchos) was the species with the greatest mortality in 1996. S
Authors
Tonie E. Rocke, Pauline Nol, C. Pelizza, K. K. Sturm
Investigations of a large scale eared grebe (Podiceps nigricollis) die-off at the Salton Sea, California in 1992
An estimated 150,000 Eared Grebes (Podiceps nigricollis) died at the Salton Sea between 16 December 1991 and 21 April 1992. This represented the largest documented mortality event of Eared Grebes at the time and approximately 6% of the North American population. During the die-off, grebes exhibited several uncharacteristic behaviors, such as congregating at freshwater tributaries, repeatedly gulpi
Authors
C.U. Meteyer, D. J. Audet, T.E. Rocke, W. Radke, L. H. Creekmore, R. Duncan
Protection of black-tailed prairie dogs (Cynomys ludovicianus) against plague after voluntary consumption of baits containing recombinant raccoon poxvirus vaccine
Prairie dogs (Cynomys spp.) are highly susceptible to Yersinia pestis and significant reservoirs of plague for humans in the western United States. A recombinant raccoon poxvirus, expressing the F1 antigen of Y. pestis, was incorporated into a palatable bait and offered to 18 black-tailed prairie dogs (Cynomys ludovicianus) for voluntary consumption; 18 negative control animals received placebo ba
Authors
J.S. Mencher, S.R. Smith, T.D. Powell, D.T. Stinchcomb, J.E. Osorio, T.E. Rocke
Could blackbird mortality from avicide DRC-1339 contribute to avian botulism outbreaks in North Dakota?
Blackbird (family lcteridae) depredation on sunflower (Helianthus annuus) crops in the prairie states of the United States has motivated the proposed use of an avicide, DRC-1339 (3-chloro-4-methylaniline), to decrease their numbers. The resulting mortality of blackbirds at wetland roosts could increase the potential of avian botulism occurring in affected marshes. To assess this possibility, we se
Authors
Diana R. Goldberg, M. D. Samuel, T.E. Rocke, K. M. Johnson, G. Linz
Prevalence of neurotoxic Clostridium botulinum type C in the gastrointestinal tracts of tilapis (Oreochromis mossambicus) in the Salton Sea
Tilapia (Oreochromis mossambicus) have been implicated as the source of type C toxin in avian botulism outbreaks in pelicans (Pelecanus erythrorhynchos, Pelecanus occidentalis californicus) at the Salton Sea in southern California (USA). We collected sick, dead, and healthy fish from various sites throughout the Sea during the summers of 1999 through 2001 and tested them for the presence of Clostr
Authors
P.J. Nol, T.E. Rocke, K. Gross, Thomas M. Yuill
Limited infection upon human exposure to a recombinant raccoon pox vaccine vector
A laboratory accident resulted in human exposure to a recombinant raccoon poxvirus (RCN) developed as a vaccine vector for antigens of Yersinia pestis for protection of wild rodents (and other animals) against plague. Within 9 days, the patient developed a small blister that healed within 4 weeks. Raccoon poxvirus was cultured from the lesion, and the patient developed antibody to plague antigen (
Authors
T.E. Rocke, F. J. Dein, M. Fuchsberger, B.C. Fox, D.T. Stinchcomb, J.G. Osorio
Recombinant F1-V fusion protein protects black-footed ferrets (Mustela nigripes) against virulent Yersinia pestis infection
Black-footed ferrets (Mustela nigripes) are highly susceptible to sylvatic plague, caused by the bacterium Yersinia pestis, and this disease has severely hampered efforts to restore ferrets to their historic range. A study was conducted to assess the efficacy of vaccination of black-footed ferrets against plague using a recombinant protein vaccine, designated F1-V, developed by personnel at the U.
Authors
Tonie E. Rocke, J. Mencher, Susan Smith, A. M. Friedlander, G.P. Andrews, L. A. Baeten
Recombinant raccoon pox vaccine protects mice against lethal plague
Using a raccoon poxvirus (RCN) expression system, we have developed new recombinant vaccines that can protect mice against lethal plague infection. We tested the effects of a translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen in tissue culture and the induction of anti
Authors
J.E. Osorio, T.D. Powell, R.S. Frank, K. Moss, E.J. Haanes, S.R. Smith, T.E. Rocke, D.T. Stinchcomb
Determination of the median toxic dose of type C botulism in lactating dairy cows
Because of the difficulty in identifying botulinum toxin in cattle, it is hypothesized that cattle are sensitive to levels of toxin below the detection limits of current diagnostic techniques (the mouse protection bioassay and the immunostick enzyme-linked immunosorbent assay [ELISA] for type C botulinum toxin). Using an up-down method for toxicologic testing, the median toxic dose (MTD50) for cat
Authors
R.B. Moeller, B. Puschner, R.L. Walker, Tonie E. Rocke, F.D. Galey, J.S. Cullor, A.A. Ardans
New host record of avian tuberculosis in an American white pelican, Pelecanus erythrorhynchos
An American white pelican (P. erythrorhyncos) was captured in August 1999 in California, USA. The bird was unable to fly away or evade the boat, and showed considerable aggression when captured. Numerous multifocal, 1 mm diameter, yellow masses were observed throughout the lungs and air sacs. The liver was yellow. A 6 cm diameter, firm spherical mass was present in the abdominal cavity, distal to
Authors
P. Nol, R.E. Brannian, B.M. Berlowski, M. J. Wolcott, T.E. Rocke
Failure to transmit avian vacuolar myelinopathy to mallard ducks
Avian vacuolar myelinopathy (AVM) is a neurologic disease that has been diagnosed in free-ranging birds in the southeastern United States. Bald eagles (Haliaeetus leuocephalus), American coots (Fulica americana), and mallards (Anas platyrhynchos) have been affected. Previous investigations have not determined the etiology of this disease. In November and December 2002, we attempted to induce AVM i
Authors
R. S. Larsen, Felicia B. Nutter, Tom Augspurger, Tonie E. Rocke, Nancy J. Thomas, Michael K. Stoskopf