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Publications

View citations of publications by National Wildlife Health Center scientists since our founding in 1975.  Access to full-text is provided where possible.

Filter Total Items: 1614

Prevalence and pathology of West Nile virus in naturally infected house sparrows, western Nebraska, 2008

Nestling birds are rarely sampled in the field for most arboviruses, yet they may be important in arbovirus amplification cycles. We sampled both nestling and adult house sparrows (Passer domesticus) in western Nebraska for West Nile virus (WNV) or WNV-specific antibodies throughout the summer of 2008 and describe pathology in naturally infected nestlings. Across the summer, 4% of nestling house s
Authors
Valerie A. O'Brien, Carol U. Meteyer, William K. Reisen, Hon S. Ip, Charles R. Brown

Avian botulism: a case study in translocated endangered Laysan ducks (Anas laysanensis) on Midway Atoll

Laysan Ducks are endemic to the Hawaiian archipelago and are one of the world’s most endangered waterfowl. For 150 yr, Laysan Ducks were restricted to an estimated 4 km2 of land on Laysan Island in the northwestern Hawaiian Islands. In 2004 and 2005, 42 Laysan Ducks were translocated to Midway Atoll, and the population increased to approximately 200 by 2007. In August 2008, mortality due to botuli
Authors
Thierry M. Work, John L. Klavitter, Michelle H. Reynolds, David S. Blehert

Testing independent and interactive effects of corticosterone and synergized resmethrin on the immune response to West Nile virus in chickens

Public health agencies utilize aerial insecticides to interrupt an active West Nile virus (WNV) transmission cycle, which may expose WNV-infected birds to these agents. Although resmethrin has been considered benign to birds, no studies have evaluated whether the environmentally employed form of resmethrin with PBO synergist (synergized resmethrin (SR)) can suppress avian immunity to WNV infection
Authors
Mark D. Jankowski, J. Christian Franson, Erich Möstl, Warren P. Porter, Erik K. Hofmeister

Consumption of baits containing raccoon pox-based plague vaccines protects black-tailed prairie dogs (Cynomys ludovicianus)

Baits containing recombinant raccoon poxvirus (RCN) expressing plague antigens (fraction 1 [F1] and a truncated form of the V protein-V307) were offered for voluntary consumption several times over the course of several months to a group of 16 black-tailed prairie dogs (Cynomys ludovicianus). For comparison, another group of prairie dogs (n = 12) was injected subcutaneously (SC) (prime and boost)
Authors
Tonie E. Rocke, Nicola Pussini, Susan Smith, Judy L. Williamson, Bradford Powell, Jorge E. Osorio

Pathology and virus detection in tissues of nestling house sparrows naturally infected with Buggy Creek virus (Togaviridae).

Alphaviruses (Togaviridae) infect wild birds, but clinical illness and death attributable to virus in naturally infected birds is rarely reported, particularly for small passerine species or nestlings. Buggy Creek virus is a unique alphavirus in the Western equine encephalomyelitis virus (WEEV) complex that is vectored by the cimicid swallow bug (Oeciacus vicarius), an ectoparasite of the colonial
Authors
Valerie A. O'Brien, Carol U. Meteyer, Hon S. Ip, Renee Romaine Long, Charles R. Brown

Limited evidence of trans-hemispheric movement of avian influenza viruses among contemporary North American shorebird isolates

Migratory routes of gulls, terns, and shorebirds (Charadriiformes) are known to cross hemispheric boundaries and intersect with outbreak areas of highly pathogenic avian influenza (HPAI). Prior assessments of low pathogenic avian influenza (LPAI) among species of this taxonomic order found some evidence for trans-hemispheric movement of virus genes. To specifically clarify the role of shorebird sp
Authors
John M. Pearce, Andrew M. Ramey, Hon S. Ip, Robert E. Gill

Wound repair in Montipora capitata

We documented the microscopic morphology of tissue healing in Montipora capitata. Fragments from two healthy coral colonies were traumatized by scraping tissue and skeleton and monitored in flow-through seawater tables every 2-4. days for 40. days for gross and cellular changes. Grossly, corals appeared healed and repigmented by Day 40. Histologically, traumatized issues were undistinguishable fro
Authors
Thierry M. Work, Greta S. Aeby

Characterization of recombinant Raccoonpox Vaccine Vectors in Chickens

Raccoonpox virus (RCN) has been used as a recombinant vector against several mammalian pathogens but has not been tested in birds. The replication of RCN in chick embryo fibroblasts (CEFs) and chickens was studied with the use of highly pathogenic avian influenza virus H5N1 hemagglutinin (HA) as a model antigen and luciferase (luc) as a reporter gene. Although RCN replicated to low levels in CEFs,
Authors
S.-H. Hwa, Keith P. Iams, Jeffrey S. Hall, B.A. Kingstad, Jorge E. Osorio

Transmission and reassortment of avian influenza viruses at the Asian-North American interface

Twenty avian influenza viruses were isolated from seven wild migratory bird species sampled at St. Lawrence Island, Alaska. We tested predictions based on previous phylogenetic analyses of avian influenza viruses that support spatially dependent trans-hemispheric gene flow and frequent interspecies transmission at a location situated at the Asian–North American interface. Through the application o
Authors
Andrew M. Ramey, John M. Pearce, Craig R. Ely, Lisa M. Sheffield Guy, David B. Irons, Dirk V. Derksen, Hon S. Ip

Validation of a real-time reverse transcriptase-PCR assay for the detection of H7 avian influenza virus

This report describes the validation of an avian influenza virus (AIV) H7 subtype-specific real-time reverse transcriptasePCR (rRT-PCR) assay developed at the Southeast Poultry Research Laboratory (SEPRL) for the detection of H7 AI in North and South American wild aquatic birds and poultry. The validation was a collaborative effort by the SEPRL and the National Veterinary Services Laboratories. Th
Authors
J. Pedersen, M.L. Killian, N. Hines, D. Senne, B. Panigrahy, Hon S. Ip, Erica Spackman

Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats

A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1–2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia per
Authors
Jeffrey M. Lorch, A. Gargas, Carol U. Meteyer, B. M. Berlowski-Zier, D. E. Green, V. Shearn-Bochsler, N. J. Thomas, David S. Blehert