Hon Ip (Former Employee)
Science and Products
Filter Total Items: 102
Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
Authors
Mariano Carossino, Hon S. Ip, Jürgen a. Richt, Kendra Schultz, Kimberly Harper, Alan T. Loynachan, Fabio Del Piero, Udeni B.R. Balasuriya
Inactivation of viable surrogates for the select agents virulent Newcastle disease virus and highly pathogenic avian influenza virus using either commercial lysis buffer or heat
Introduction:Federal Select Agent Program regulations require laboratories to document a validated procedure for inactivating select agents prior to movement outside registered space. Avian influenza viruses and virulent Newcastle disease virus (vNDV) are cultured in chicken amnio-allantoic fluid (AAF), but the efficacy of commercial lysis buffers to inactivate viruses in protein-rich...
Authors
Katrina E. Alger, Hon S. Ip, Jeffrey S. Hall, Sean Nashold, Katherine Richgels, Carrie Alison Smith
Artificial intelligence and avian influenza: Using machine learning to enhance active surveillance for avian influenza viruses
Influenza A viruses are one of the most significant viral groups globally with substantial impacts on human, domestic animal and wildlife health. Wild birds are the natural reservoirs for these viruses, and active surveillance within wild bird populations provides critical information about viral evolution forming the basis of risk assessments and countermeasure development...
Authors
Daniel P. Walsh, Ting Fung Ma, Hon S. Ip, Jun Zhu
Lethal infection of wild raptors with highly pathogenic avian influenza H5N8 and H5N2 viruses in the USA, 2014–15
An outbreak of highly pathogenic avian influenza (HPAI) led to heavy losses of poultry in commercial farms in North America in 2014–15. Enhanced surveillance by virologists and pathologists at the US Geological Survey National Wildlife Health Center and its partners resulted in the identification of lethal infections with clade 2.3.4.4 subgroup icA2 H5N8 and novel reassortant H5N2...
Authors
Susan Knowles, Valerie I. Shearn-Bochsler, Hon S. Ip
Inferring epidemiologic dynamics from viral evolution: 2014–2015 Eurasian/North American highly pathogenic avian influenza viruses exceed transmission threshold, R0 = 1, in wild birds and poultry in North America
Highly pathogenic avian influenza virus (HPAIV) is a multihost pathogen with lineages that pose health risks for domestic birds, wild birds, and humans. One mechanism of intercontinental HPAIV spread is through wild bird reservoirs, and wild birds were the likely sources of a Eurasian (EA) lineage HPAIV into North America in 2014. The introduction resulted in several reassortment events...
Authors
Daniel R. Grear, Jeffrey S. Hall, Robert J. Dusek, Hon S. Ip
Experimental infection of common eider ducklings with Wellfleet Bay virus, a newly characterized orthomyxovirus
Wellfleet Bay virus (WFBV), a novel orthomyxovirus in the genus Quaranjavirus, was first isolated in 2006 from carcasses of common eider (Somateria mollissima) during a mortality event in Wellfleet Bay (Barnstable County, Massachusetts, USA) and has since been repeatedly isolated during recurrent mortality events in this location. Hepatic, pancreatic, splenic, and intestinal necrosis...
Authors
Valerie I. Shearn-Bochsler, Hon S. Ip, Anne Ballmann, Jeffrey S. Hall, Andrew B. Allison, Jennifer R. Ballard, Julie C. Ellis, Robert Cook, Samantha E. J. Gibbs, Chris P. Dwyer
Natural infections with pigeon paramyxovirus serotype 1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the United States
Pigeon paramyxovirus serotype 1 (PPMV-1) is a globally distributed, virulent member of the avian paramyxovirus serotype 1 serogroup that causes mortality in columbiformes and poultry. Following introduction into the United States in the mid-1980s, PPMV-1 rapidly spread causing numerous mortality events in Eurasian collared-doves (Streptopelia decaocto) (ECDOs) and rock pigeons (Columba...
Authors
Marcos Isidoro Ayza, C.L. Afonso, J.B. Stanton, Susan Knowles, Hon S. Ip, C. LeAnn White, Heather Fenton, M.G. Ruder, A. C. Dolinski, Julia S. Lankton
Identification of two novel reassortant avian influenza a (H5N6) viruses in whooper swans in Korea, 2016
BackgroundOn November 20, 2016 two novel strains of H5N6 highly pathogenic avian influenza virus (HPAIVs) were isolated from three whooper swans (Cygnus cygnus) at Gangjin Bay in South Jeolla province, South Korea. Identification of HPAIVs in wild birds is significant as there is a potential risk of transmission of these viruses to poultry and humans.ResultsPhylogenetic analysis revealed...
Authors
Jipseol Jeong, Chanjin Woo, Hon S. Ip, Injung An, Youngsik Kim, Kwanghee Lee, Seong-Deok Jo, Kidong Son, Saemi Lee, Jae-Ku Oem, Seung-Jun Wang, Yongkwan Kim, Jeonghwa Shin, Jonathan M. Sleeman, Weonhwa Jheong
Detection of spring viraemia of carp virus in imported amphibians reveals an unanticipated foreign animal disease threat
Global translocation of plants and animals is a well-recognized mechanism for introduction of pathogens into new regions. To mitigate this risk, various tools such as preshipment health certificates, quarantines, screening for specific disease agents and outright bans have been implemented. However, such measures only target known infectious agents and their hosts and may fail to prevent...
Authors
Hon S. Ip, Jeffrey M. Lorch, David S. Blehert
H9N2 influenza A virus isolated from a Greater White-fronted wild goose (Anser albifrons) in Alaska has a mutation in the PB2 gene, which is associated with pathogenicity in human pandemic 2009 H1N1
We report here the genomic sequence of an H9N2 influenza A virus [A/greater white-fronted goose/Alaska/81081/2008 (H9N2)]. This virus shares ≥99.8% identity with a previously reported virus. Both strains contain a G590S mutation in the polymerase basic 2 (PB2) gene, which is a pathogenicity marker in the pandemic 2009 H1N1 virus when combined with R591.
Authors
Andrew B. Reeves, Hon S. Ip
U.S. Geological Survey science strategy for highly pathogenic avian influenza in wildlife and the environment (2016–2020)
IntroductionThrough the Science Strategy for Highly Pathogenic Avian Influenza (HPAI) in Wildlife and the Environment, the USGS will assess avian influenza (AI) dynamics in an ecological context to inform decisions made by resource managers and policymakers from the local to national level. Through collection of unbiased scientific information on the ecology of AI viruses and wildlife...
Authors
M. Camille Harris, John M. Pearce, Diann J. Prosser, C. LeAnn White, A. Keith Miles, Jonathan M. Sleeman, Christopher J. Brand, James P. Cronin, Susan De La Cruz, Christine L. Densmore, Thomas W. Doyle, Robert J. Dusek, Joseph P. Fleskes, Paul L. Flint, Gerald F. Guala, Jeffrey S. Hall, Laura E. Hubbard, Randall J. Hunt, Hon S. Ip, Rachel A. Katz, Kevin W. Laurent, Mark P. Miller, Mark D. Munn, Andrew M. Ramey, Kevin D. Richards, Robin E. Russell, Joel P. Stokdyk, John Y. Takekawa, Daniel P. Walsh
Widespread detection of highly pathogenic H5 influenza viruses in wild birds from the Pacific Flyway of the United States
A novel highly pathogenic avian influenza virus belonging to the H5 clade 2.3.4.4 variant viruses was detected in North America in late 2014. Motivated by the identification of these viruses in domestic poultry in Canada, an intensive study was initiated to conduct highly pathogenic avian influenza surveillance in wild birds in the Pacific Flyway of the United States. A total of 4,729...
Authors
S.N. Bevins, Robert J. Dusek, C. LeAnn White, Thomas Gidlewski, B. Bodenstein, Kristin G. Mansfield, Paul DeBruyn, Donald K. Kraege, E. L. Rowan, Colin Gillin, B. Thomas, S. Chandler, J. Baroch, B. Schmit, M. J. Grady, R. S. Miller, M.L. Drew, S. Stopak, B. Zscheile, J. Bennett, J. Sengl, Caroline Brady, Hon S. Ip, Erica Spackman, M. L. Killian, Mia Kim Torchetti, Jonathan M. Sleeman, T.J. DeLiberto
Science and Products
Filter Total Items: 102
Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
Authors
Mariano Carossino, Hon S. Ip, Jürgen a. Richt, Kendra Schultz, Kimberly Harper, Alan T. Loynachan, Fabio Del Piero, Udeni B.R. Balasuriya
Inactivation of viable surrogates for the select agents virulent Newcastle disease virus and highly pathogenic avian influenza virus using either commercial lysis buffer or heat
Introduction:Federal Select Agent Program regulations require laboratories to document a validated procedure for inactivating select agents prior to movement outside registered space. Avian influenza viruses and virulent Newcastle disease virus (vNDV) are cultured in chicken amnio-allantoic fluid (AAF), but the efficacy of commercial lysis buffers to inactivate viruses in protein-rich...
Authors
Katrina E. Alger, Hon S. Ip, Jeffrey S. Hall, Sean Nashold, Katherine Richgels, Carrie Alison Smith
Artificial intelligence and avian influenza: Using machine learning to enhance active surveillance for avian influenza viruses
Influenza A viruses are one of the most significant viral groups globally with substantial impacts on human, domestic animal and wildlife health. Wild birds are the natural reservoirs for these viruses, and active surveillance within wild bird populations provides critical information about viral evolution forming the basis of risk assessments and countermeasure development...
Authors
Daniel P. Walsh, Ting Fung Ma, Hon S. Ip, Jun Zhu
Lethal infection of wild raptors with highly pathogenic avian influenza H5N8 and H5N2 viruses in the USA, 2014–15
An outbreak of highly pathogenic avian influenza (HPAI) led to heavy losses of poultry in commercial farms in North America in 2014–15. Enhanced surveillance by virologists and pathologists at the US Geological Survey National Wildlife Health Center and its partners resulted in the identification of lethal infections with clade 2.3.4.4 subgroup icA2 H5N8 and novel reassortant H5N2...
Authors
Susan Knowles, Valerie I. Shearn-Bochsler, Hon S. Ip
Inferring epidemiologic dynamics from viral evolution: 2014–2015 Eurasian/North American highly pathogenic avian influenza viruses exceed transmission threshold, R0 = 1, in wild birds and poultry in North America
Highly pathogenic avian influenza virus (HPAIV) is a multihost pathogen with lineages that pose health risks for domestic birds, wild birds, and humans. One mechanism of intercontinental HPAIV spread is through wild bird reservoirs, and wild birds were the likely sources of a Eurasian (EA) lineage HPAIV into North America in 2014. The introduction resulted in several reassortment events...
Authors
Daniel R. Grear, Jeffrey S. Hall, Robert J. Dusek, Hon S. Ip
Experimental infection of common eider ducklings with Wellfleet Bay virus, a newly characterized orthomyxovirus
Wellfleet Bay virus (WFBV), a novel orthomyxovirus in the genus Quaranjavirus, was first isolated in 2006 from carcasses of common eider (Somateria mollissima) during a mortality event in Wellfleet Bay (Barnstable County, Massachusetts, USA) and has since been repeatedly isolated during recurrent mortality events in this location. Hepatic, pancreatic, splenic, and intestinal necrosis...
Authors
Valerie I. Shearn-Bochsler, Hon S. Ip, Anne Ballmann, Jeffrey S. Hall, Andrew B. Allison, Jennifer R. Ballard, Julie C. Ellis, Robert Cook, Samantha E. J. Gibbs, Chris P. Dwyer
Natural infections with pigeon paramyxovirus serotype 1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the United States
Pigeon paramyxovirus serotype 1 (PPMV-1) is a globally distributed, virulent member of the avian paramyxovirus serotype 1 serogroup that causes mortality in columbiformes and poultry. Following introduction into the United States in the mid-1980s, PPMV-1 rapidly spread causing numerous mortality events in Eurasian collared-doves (Streptopelia decaocto) (ECDOs) and rock pigeons (Columba...
Authors
Marcos Isidoro Ayza, C.L. Afonso, J.B. Stanton, Susan Knowles, Hon S. Ip, C. LeAnn White, Heather Fenton, M.G. Ruder, A. C. Dolinski, Julia S. Lankton
Identification of two novel reassortant avian influenza a (H5N6) viruses in whooper swans in Korea, 2016
BackgroundOn November 20, 2016 two novel strains of H5N6 highly pathogenic avian influenza virus (HPAIVs) were isolated from three whooper swans (Cygnus cygnus) at Gangjin Bay in South Jeolla province, South Korea. Identification of HPAIVs in wild birds is significant as there is a potential risk of transmission of these viruses to poultry and humans.ResultsPhylogenetic analysis revealed...
Authors
Jipseol Jeong, Chanjin Woo, Hon S. Ip, Injung An, Youngsik Kim, Kwanghee Lee, Seong-Deok Jo, Kidong Son, Saemi Lee, Jae-Ku Oem, Seung-Jun Wang, Yongkwan Kim, Jeonghwa Shin, Jonathan M. Sleeman, Weonhwa Jheong
Detection of spring viraemia of carp virus in imported amphibians reveals an unanticipated foreign animal disease threat
Global translocation of plants and animals is a well-recognized mechanism for introduction of pathogens into new regions. To mitigate this risk, various tools such as preshipment health certificates, quarantines, screening for specific disease agents and outright bans have been implemented. However, such measures only target known infectious agents and their hosts and may fail to prevent...
Authors
Hon S. Ip, Jeffrey M. Lorch, David S. Blehert
H9N2 influenza A virus isolated from a Greater White-fronted wild goose (Anser albifrons) in Alaska has a mutation in the PB2 gene, which is associated with pathogenicity in human pandemic 2009 H1N1
We report here the genomic sequence of an H9N2 influenza A virus [A/greater white-fronted goose/Alaska/81081/2008 (H9N2)]. This virus shares ≥99.8% identity with a previously reported virus. Both strains contain a G590S mutation in the polymerase basic 2 (PB2) gene, which is a pathogenicity marker in the pandemic 2009 H1N1 virus when combined with R591.
Authors
Andrew B. Reeves, Hon S. Ip
U.S. Geological Survey science strategy for highly pathogenic avian influenza in wildlife and the environment (2016–2020)
IntroductionThrough the Science Strategy for Highly Pathogenic Avian Influenza (HPAI) in Wildlife and the Environment, the USGS will assess avian influenza (AI) dynamics in an ecological context to inform decisions made by resource managers and policymakers from the local to national level. Through collection of unbiased scientific information on the ecology of AI viruses and wildlife...
Authors
M. Camille Harris, John M. Pearce, Diann J. Prosser, C. LeAnn White, A. Keith Miles, Jonathan M. Sleeman, Christopher J. Brand, James P. Cronin, Susan De La Cruz, Christine L. Densmore, Thomas W. Doyle, Robert J. Dusek, Joseph P. Fleskes, Paul L. Flint, Gerald F. Guala, Jeffrey S. Hall, Laura E. Hubbard, Randall J. Hunt, Hon S. Ip, Rachel A. Katz, Kevin W. Laurent, Mark P. Miller, Mark D. Munn, Andrew M. Ramey, Kevin D. Richards, Robin E. Russell, Joel P. Stokdyk, John Y. Takekawa, Daniel P. Walsh
Widespread detection of highly pathogenic H5 influenza viruses in wild birds from the Pacific Flyway of the United States
A novel highly pathogenic avian influenza virus belonging to the H5 clade 2.3.4.4 variant viruses was detected in North America in late 2014. Motivated by the identification of these viruses in domestic poultry in Canada, an intensive study was initiated to conduct highly pathogenic avian influenza surveillance in wild birds in the Pacific Flyway of the United States. A total of 4,729...
Authors
S.N. Bevins, Robert J. Dusek, C. LeAnn White, Thomas Gidlewski, B. Bodenstein, Kristin G. Mansfield, Paul DeBruyn, Donald K. Kraege, E. L. Rowan, Colin Gillin, B. Thomas, S. Chandler, J. Baroch, B. Schmit, M. J. Grady, R. S. Miller, M.L. Drew, S. Stopak, B. Zscheile, J. Bennett, J. Sengl, Caroline Brady, Hon S. Ip, Erica Spackman, M. L. Killian, Mia Kim Torchetti, Jonathan M. Sleeman, T.J. DeLiberto
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government