Luke R Iwanowicz, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 13
Filter Total Items: 22
No Result Found
Filter Total Items: 103
Utilization of protein expression profiles as indicators of environmental impairment of smallmouth bass (Micropterus dolomieu) from the Shenandoah River, Virginia, USA
The Shenandoah River (VA, USA), the largest tributary of the Potomac River (MD, USA) and an important source of drinking water, has been the site of extensive fish kills since 2004. Previous investigations indicate environmental stressors may be adversely modulating the immune system of smallmouth bass (Micropterus dolomieu) and other species. Anterior kidney (AK) tissue, the major site of blood c
Authors
J. Ripley, L. Iwanowicz, V. Blazer, C. Foran
Intersex (Testicular Oocytes) in smallmouth bass from the Potomac River and selected nearby drainages
Intersex, or the presence of characteristics of both sexes, in fishes that are normally gonochoristic has been used as an indicator of exposure to estrogenic compounds. In 2003, during health assessments conducted in response to kills and a high prevalence of skin lesions observed in smallmouth bass Micropterus dolomieu in the South Branch of the Potomac River, the presence of immature oocytes wit
Authors
Vicki S. Blazer, Luke R. Iwanowicz, Deborah D. Iwanowicz, David R. Smith, John A. Young, J.D. Hedrick, S.W. Foster, S.J. Reeser
Fish health study Ashtabula River natural resource damage assessment
INTRODUCTION
The Ashtabula River is located in northeast Ohio, flowing into Lake Erie at Ashtabula, Ohio. Tributaries include Fields Brook, Hubbard Run, Strong Brook, and Ashtabula Creek. The bottom sediments, bank soils and biota of Fields Brook have been severely contaminated by unregulated discharges of hazardous substances. Hazardous substances have migrated downstream from Fields Brook to
Authors
V. S. Blazer, L. R. Iwanowicz, P. C. Baumann
Mortality and pathology in brown bullheads Amieurus nebulosus associated with a spontaneous Edwardsiella ictaluri outbreak under tank culture conditions
Brown bullheads Amieurus nebulosus (family Ictaluridae) are commonly used as a sentinel of environmental contamination. These fish are not generally cultured under laboratory conditions and little is known about their disease susceptibility. Here we report an outbreak of disease due to Edwardsiella ictaluri in a laboratory population of tank-reared, wild-caught brown bullheads. The isolate was pos
Authors
L. R. Iwanowicz, A.R. Griffin, Deborah D. Cartwright, V. S. Blazer
Aqueous exposure to Aroclor 1254 modulates the mitogenic response of Atlantic salmon anterior kidney T-cells: Indications of short- and long-term immunomodulation
Polychlorinated biphenyls (PCBs) exist as persistent organic pollutants in numerous river systems in the United States. Unfortunately, some of these rivers are sites of active Atlantic salmon restoration programs, and polychlorinated biphenyls have been implicated as ancillary factors contributing to failed salmon restoration. Here, we investigate the immediate and chronic effects of intermediate
Authors
L. R. Iwanowicz, D.T. Lerner, V. S. Blazer, S. D. McCormick
Calcein AM release-based cytotoxic cell assay for fish leucocytes
A non-specific cytotoxic cell assay for fish is presented that is based on the release of the activated fluorochrome calcein AM from lysed carp epithelioma papulosum cyprini (EPC) cells. To establish the suitability of treating EPC cells with calcein AM the uptake and spontaneous release of the calcein AM by the EPC cells was evaluated. Incubation of 5 μM calcein AM in culture medium with 1×105EPC
Authors
Luke R. Iwanowicz, Christine L. Densmore, Christopher A. Ottinger
Immunomodulation and disease resistance in postyearling rainbow trout infected with Myxobolus cerebralis, the causative agent of whirling disease
Myxobolus cerebralis, the myxosporean parasite that causes whirling disease, has a number of deleterious effects on its salmonid host. Although it is well established that juvenile salmonids in the active stages of whirling disease mount an immune response to the pathogen, the occurrence and longevity of any related immunomodulatory effects are unknown. In this study, postyearling rainbow trout On
Authors
Christine L. Densmore, Christopher A. Ottinger, Vicki S. Blazer, Luke R. Iwanowicz, D. R. Smith
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 13
Filter Total Items: 22
No Result Found
Filter Total Items: 103
Utilization of protein expression profiles as indicators of environmental impairment of smallmouth bass (Micropterus dolomieu) from the Shenandoah River, Virginia, USA
The Shenandoah River (VA, USA), the largest tributary of the Potomac River (MD, USA) and an important source of drinking water, has been the site of extensive fish kills since 2004. Previous investigations indicate environmental stressors may be adversely modulating the immune system of smallmouth bass (Micropterus dolomieu) and other species. Anterior kidney (AK) tissue, the major site of blood c
Authors
J. Ripley, L. Iwanowicz, V. Blazer, C. Foran
Intersex (Testicular Oocytes) in smallmouth bass from the Potomac River and selected nearby drainages
Intersex, or the presence of characteristics of both sexes, in fishes that are normally gonochoristic has been used as an indicator of exposure to estrogenic compounds. In 2003, during health assessments conducted in response to kills and a high prevalence of skin lesions observed in smallmouth bass Micropterus dolomieu in the South Branch of the Potomac River, the presence of immature oocytes wit
Authors
Vicki S. Blazer, Luke R. Iwanowicz, Deborah D. Iwanowicz, David R. Smith, John A. Young, J.D. Hedrick, S.W. Foster, S.J. Reeser
Fish health study Ashtabula River natural resource damage assessment
INTRODUCTION
The Ashtabula River is located in northeast Ohio, flowing into Lake Erie at Ashtabula, Ohio. Tributaries include Fields Brook, Hubbard Run, Strong Brook, and Ashtabula Creek. The bottom sediments, bank soils and biota of Fields Brook have been severely contaminated by unregulated discharges of hazardous substances. Hazardous substances have migrated downstream from Fields Brook to
Authors
V. S. Blazer, L. R. Iwanowicz, P. C. Baumann
Mortality and pathology in brown bullheads Amieurus nebulosus associated with a spontaneous Edwardsiella ictaluri outbreak under tank culture conditions
Brown bullheads Amieurus nebulosus (family Ictaluridae) are commonly used as a sentinel of environmental contamination. These fish are not generally cultured under laboratory conditions and little is known about their disease susceptibility. Here we report an outbreak of disease due to Edwardsiella ictaluri in a laboratory population of tank-reared, wild-caught brown bullheads. The isolate was pos
Authors
L. R. Iwanowicz, A.R. Griffin, Deborah D. Cartwright, V. S. Blazer
Aqueous exposure to Aroclor 1254 modulates the mitogenic response of Atlantic salmon anterior kidney T-cells: Indications of short- and long-term immunomodulation
Polychlorinated biphenyls (PCBs) exist as persistent organic pollutants in numerous river systems in the United States. Unfortunately, some of these rivers are sites of active Atlantic salmon restoration programs, and polychlorinated biphenyls have been implicated as ancillary factors contributing to failed salmon restoration. Here, we investigate the immediate and chronic effects of intermediate
Authors
L. R. Iwanowicz, D.T. Lerner, V. S. Blazer, S. D. McCormick
Calcein AM release-based cytotoxic cell assay for fish leucocytes
A non-specific cytotoxic cell assay for fish is presented that is based on the release of the activated fluorochrome calcein AM from lysed carp epithelioma papulosum cyprini (EPC) cells. To establish the suitability of treating EPC cells with calcein AM the uptake and spontaneous release of the calcein AM by the EPC cells was evaluated. Incubation of 5 μM calcein AM in culture medium with 1×105EPC
Authors
Luke R. Iwanowicz, Christine L. Densmore, Christopher A. Ottinger
Immunomodulation and disease resistance in postyearling rainbow trout infected with Myxobolus cerebralis, the causative agent of whirling disease
Myxobolus cerebralis, the myxosporean parasite that causes whirling disease, has a number of deleterious effects on its salmonid host. Although it is well established that juvenile salmonids in the active stages of whirling disease mount an immune response to the pathogen, the occurrence and longevity of any related immunomodulatory effects are unknown. In this study, postyearling rainbow trout On
Authors
Christine L. Densmore, Christopher A. Ottinger, Vicki S. Blazer, Luke R. Iwanowicz, D. R. Smith
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government