Tariq Tajjioui
Tariq Tajjioui
Science and Products
Milwaukee River environmental DNA data for bigheaded carp and four other species from 2021 and 2022
An eDNA-based sampling approach used by the U.S. Fish and Wildlife Service for bigheaded carp Hypophthalmichthys spp. in the upper Mississippi River and Great Lakes Basins that collects hundreds of water samples per event. The U.S. Fish and Wildlife Service detected a single positive sample for bighead carp _H. nobilis_ during the spring 2021 sampling event in the Kinnickinnic River...
Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River
This dataset contains raw sequence data collected from an eDNA metabarcoding project to detect freshwater mussel species across two sites in Georgia (Spring Creek and Flint River) and one drainage in Missouri (Big Piney River). The eDNA samples were collected from each stream using dead-end ultra filtration (D-HFUF) with eDNA extracted from filters. We used two previously published...
Data Release for Validation of portable eDNA detection kit for Asian carps
This is the qPCR and loop-mediated isothermal amplification (LAMP) data in support of the article "Validation of a portable eDNA detection kit for invasive carps". There are four types of data contained in five files including: limit of detection analysis for each method (qPCR and LAMP), qPCR analysis and LAMP analysis of eDNA samples collected over time from ponds containing 3 or 33...
Dead-end hollow fiber ultrafiltration capture of environmental DNA for freshwater mussel (Unionidae) species detection with metabarcoding
Insufficient water sample volumes can be a limiting factor for detecting species with environmental DNA (eDNA) from aquatic habitats. We compared detections of freshwater mussel (Unionidae) communities using large water sample volumes and dead-end hollow fiber ultrafiltration (D-HFUF or DEUF) with traditional eDNA filtration methods that use relatively small water sample volumes. Unionid...
Authors
Anna M. McKee, Katy E. Klymus, Yer Lor, Marissa H Kaminski, Tariq Tajjioui, Nathan Johnson, Matthew Carroll, Christopher Goodson, Stephen Frank Spear
The relative importance of cisco (Coregonus artedi) in the diets of common loons (Gavia immer) among a set of cisco refuge lakes in Minnesota
Common loon (Gavia immer (Brünnich, 1764)) foraging patterns and the relative importance of cisco (Coregonus artedi Lesueur, 1818) in the diets of loons were evaluated for the Whitefish Chain of Lakes, a set of coldwater cisco refuge lakes in Minnesota, USA. Environmental DNA metabarcoding of loon fecal samples detected 15 fish species. Yellow perch (Perca flavescens (Mitchill, 1814))...
Authors
Kevin P. Kenow, Yer Lor, Beth V. Holbrook, Luke Fara, Steven C. Houdek, Tariq Tajjioui, Brian R. Gray, Peter C. Jacobson
Validation of a portable eDNA detection kit for invasive carps
Loop-mediated isothermal amplification (LAMP) is a rapid molecular detection technique that has been used as a diagnostic tool for detecting human and animal pathogens for over 20 years and is promising for detecting environmental DNA shed by invasive species. We designed a LAMP assay to detect the invasive carps, silver carp (Hypophthalmichthys molitrix), bighead carp...
Authors
Stacie A. Kageyama, Matthew Regh Hoogland, Tariq Tajjioui, Theresa M. Schreier, Richard A. Erickson, Christopher M. Merkes
Environmental DNA metabarcoding as a tool for biodiversity assessment and monitoring: Reconstructing established fish communities of north-temperate lakes and rivers
AimTo evaluate the ability of precipitation-based environmental DNA (eDNA) sample collection and mitochondrial 12S metabarcoding sequencing to reconstruct well-studied fish communities in lakes and rivers. Specific objectives were to 1) determine correlations between eDNA species detections and known community composition based on conventional field sampling, 2) compare efficiency of...
Authors
Peter T. Euclide, Yer Lor, Michael J. Spear, Tariq Tajjioui, Jake Vander Zanden, Wesley Larson, Jon Amberg
Development of a quantitative PCR method for screening ichthyoplankton samples for bigheaded carps
Monitoring ichthyoplankton is useful for identifying reproductive fronts and spawning locations of bigheaded carps (Hypophthalmichthys spp.). Unfortunately, sorting and identifying ichthyoplankton to monitor for bigheaded carp reproduction is time consuming and expensive. Traditional methods require frequent egg-larvae sampling, sorting of all samples to obtain presumptively identified...
Authors
Andrea K. Fritts, Brent C. Knights, James H. Larson, Jon Amberg, Christopher M. Merkes, Tariq Tajjioui, Steven E. Butler, Matthew J. Diana, David B. Wahl, Michael J. Weber, John D. Waters
Science and Products
Milwaukee River environmental DNA data for bigheaded carp and four other species from 2021 and 2022
An eDNA-based sampling approach used by the U.S. Fish and Wildlife Service for bigheaded carp Hypophthalmichthys spp. in the upper Mississippi River and Great Lakes Basins that collects hundreds of water samples per event. The U.S. Fish and Wildlife Service detected a single positive sample for bighead carp _H. nobilis_ during the spring 2021 sampling event in the Kinnickinnic River...
Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River
This dataset contains raw sequence data collected from an eDNA metabarcoding project to detect freshwater mussel species across two sites in Georgia (Spring Creek and Flint River) and one drainage in Missouri (Big Piney River). The eDNA samples were collected from each stream using dead-end ultra filtration (D-HFUF) with eDNA extracted from filters. We used two previously published...
Data Release for Validation of portable eDNA detection kit for Asian carps
This is the qPCR and loop-mediated isothermal amplification (LAMP) data in support of the article "Validation of a portable eDNA detection kit for invasive carps". There are four types of data contained in five files including: limit of detection analysis for each method (qPCR and LAMP), qPCR analysis and LAMP analysis of eDNA samples collected over time from ponds containing 3 or 33...
Dead-end hollow fiber ultrafiltration capture of environmental DNA for freshwater mussel (Unionidae) species detection with metabarcoding
Insufficient water sample volumes can be a limiting factor for detecting species with environmental DNA (eDNA) from aquatic habitats. We compared detections of freshwater mussel (Unionidae) communities using large water sample volumes and dead-end hollow fiber ultrafiltration (D-HFUF or DEUF) with traditional eDNA filtration methods that use relatively small water sample volumes. Unionid...
Authors
Anna M. McKee, Katy E. Klymus, Yer Lor, Marissa H Kaminski, Tariq Tajjioui, Nathan Johnson, Matthew Carroll, Christopher Goodson, Stephen Frank Spear
The relative importance of cisco (Coregonus artedi) in the diets of common loons (Gavia immer) among a set of cisco refuge lakes in Minnesota
Common loon (Gavia immer (Brünnich, 1764)) foraging patterns and the relative importance of cisco (Coregonus artedi Lesueur, 1818) in the diets of loons were evaluated for the Whitefish Chain of Lakes, a set of coldwater cisco refuge lakes in Minnesota, USA. Environmental DNA metabarcoding of loon fecal samples detected 15 fish species. Yellow perch (Perca flavescens (Mitchill, 1814))...
Authors
Kevin P. Kenow, Yer Lor, Beth V. Holbrook, Luke Fara, Steven C. Houdek, Tariq Tajjioui, Brian R. Gray, Peter C. Jacobson
Validation of a portable eDNA detection kit for invasive carps
Loop-mediated isothermal amplification (LAMP) is a rapid molecular detection technique that has been used as a diagnostic tool for detecting human and animal pathogens for over 20 years and is promising for detecting environmental DNA shed by invasive species. We designed a LAMP assay to detect the invasive carps, silver carp (Hypophthalmichthys molitrix), bighead carp...
Authors
Stacie A. Kageyama, Matthew Regh Hoogland, Tariq Tajjioui, Theresa M. Schreier, Richard A. Erickson, Christopher M. Merkes
Environmental DNA metabarcoding as a tool for biodiversity assessment and monitoring: Reconstructing established fish communities of north-temperate lakes and rivers
AimTo evaluate the ability of precipitation-based environmental DNA (eDNA) sample collection and mitochondrial 12S metabarcoding sequencing to reconstruct well-studied fish communities in lakes and rivers. Specific objectives were to 1) determine correlations between eDNA species detections and known community composition based on conventional field sampling, 2) compare efficiency of...
Authors
Peter T. Euclide, Yer Lor, Michael J. Spear, Tariq Tajjioui, Jake Vander Zanden, Wesley Larson, Jon Amberg
Development of a quantitative PCR method for screening ichthyoplankton samples for bigheaded carps
Monitoring ichthyoplankton is useful for identifying reproductive fronts and spawning locations of bigheaded carps (Hypophthalmichthys spp.). Unfortunately, sorting and identifying ichthyoplankton to monitor for bigheaded carp reproduction is time consuming and expensive. Traditional methods require frequent egg-larvae sampling, sorting of all samples to obtain presumptively identified...
Authors
Andrea K. Fritts, Brent C. Knights, James H. Larson, Jon Amberg, Christopher M. Merkes, Tariq Tajjioui, Steven E. Butler, Matthew J. Diana, David B. Wahl, Michael J. Weber, John D. Waters