Publications

Cell-mediated immunity plays an essential role for the control and eradication of intracellular pathogens. To learn more about the evolutionary origins of the first signal (Signal 1) for T-cell activation, we cloned CD8α from an elasmobranch, Rhinobatos productus. Similar to full-length CD8α cDNAs from other vertebrates, Rhpr-CD8α (1800 bp) encodes a 219 amino acid open reading frame composed of a

The plasma of Pacific herring Clupea pallasii that survived laboratory-induced viral hemorrhagic septicemia (VHS) epizootics contained humoral substances that, when injected into naive animals, conferred passive immunity against the disease. Among groups exposed to viral hemorrhagic septicemia virus (VHSV), injection of donor plasma from VHS survivors resulted in significantly greater survival (50

This study focuses on the use by juvenile Chinook salmon Oncorhynchus tshawytscha of the rarely studied neritic environment (surface waters overlaying the sublittoral zone) in greater Puget Sound. Juvenile Chinook salmon inhabit the sound from their late estuarine residence and early marine transition to their first year at sea. We measured the density, origin, and size of marked (known hatchery)

Procedures for a viral replication in excised fin tissue (VREFT) assay were adapted to Pacific herring, Clupea pallasii, and optimized both to reduce processing time and to provide the greatest resolution between na??ve herring and those previously exposed to viral haemorrhagic septicaemia virus (VHSV), Genogroup IVa. The optimized procedures included removal of the left pectoral fin from a euthan

Radio-tagged adult Pacific lamprey Entosphenus tridentatus held in a raceway with Plexiglas-lined walls and bottom healed more slowly and retained sutures longer than fish held in an all-concrete raceway or one with Plexiglas walls and a cobble-lined bottom. On all substrata, healing depended on when sutures were lost, and fish that lost their sutures in <14 days post-surgery healed faster than th

To understand the effects of increased sediment supply from dam removal on marine habitats around the Elwha River delta, a basic understanding of the region’s coastal processes is necessary. This chapter provides a summary of the physical setting of the coast near the Elwha River delta, for the purpose of synthesizing the processes that move and disperse sediment discharged by the river. One funda

During and after the planned incremental removal of two large, century-old concrete dams between 2011 and 2014, the sediment-transport regime in the lower Elwha River of western Washington will initially spike above background levels and then return to pre-dam conditions some years after complete dam removal. Measurements indicate the upper reaches of the steep-gradient Elwha River, draining the n

The removal of two dams on the Elwha River will introduce massive volumes of sediment to the river, and this increase in sediment supply in the river will likely modify the shapes and forms of the river and coastal landscape downstream of the dams. This chapter provides the geologic and geomorphologic background of the Olympic Peninsula and the Elwha River with emphasis on the present river and sh

Zebra mussels (Dreissena polymorpha) were first detected in the Great Lakes in 1988. They were likely transported as larvae or young adults inside the ballast tanks of large ocean-going ships originating from Europe. Since their introduction, they have spread throughout the Eastern, Midwestern, and Southern United States. In 2007, Quagga mussels (Dreissena rostriformis bugensis) were found in the

Laboratory challenges using specific-pathogen-free Pacific herring Clupea pallasii from three distinct populations indicated that stock origin had no effect on susceptibility to viral hemorrhagic septicemia (VHS). All of the populations were highly susceptible to the disease upon initial exposure, with significantly greater cumulative mortalities occurring in the exposed treatment groups (56.3-64.

Lost River sucker (Deltistes luxatus) and shortnose sucker (Chasmistes brevirostris) were listed as endangered in 1988 for a variety of reasons including apparent recruitment failure. Upper Klamath Lake, Oregon, and its tributaries are considered the most critical remaining habitat for these two species. Age-0 suckers are often abundant in Upper Klamath Lake throughout the summer months, but catch

The Nisqually Fall Chinook population is one of 27 salmon stocks in the Puget Sound (Washington) evolutionarily significant unit listed as threatened under the federal Endangered Species Act (ESA). Extensive restoration of the Nisqually River delta ecosystem is currently taking place to assist in recovery of the stock as juvenile Fall Chinook salmon are dependent on the estuary. A pre-restoration