Publications
USGS divers in the Elwha River
WFRC zebrafish laboratory
Processing a Lost River sucker
Below is a list of available WFRC peer reviewed and published science.
Filter Total Items: 2489
Faunal assemblages and multi-scale habitat patterns in headwater tributaries of the South Fork Trinity River - an unregulated river embedded within a multiple-use landscape
Headwaters can represent 80% of stream kilometers in a watershed, and they also have unique physical and biological properties that have only recently been recognized for their importance in sustaining healthy functioning stream networks and their ecological services. We sampled 60 headwater tributaries in the South Fork Trinity River, a 2,430 km2, mostly forested, multiple-use watershed in northw
Authors
H.H. Welsh, G.R. Hodgson, J.J. Duda, J.M. Emlen
Quantifying the behavioral response of spawning chum salmon to elevated discharges from Bonneville Dam, Columbia River, USA
Chum salmon Oncorhynchus keta that spawn in main-stem habitats below Bonneville Dam on the Columbia River, USA, are periodically subjected to elevated discharges that may alter spawning behaviour. We investigated behavioural responses of spawning chum salmon to increased water velocities associated with experimental increases in tailwater elevation using acoustic telemetry and a dual-frequency ide
Authors
K.F. Tiffan, C. A. Haskell, T.J. Kock
Early viral replication and induced or constitutive immunity in rainbow trout families with differential resistance to Infectious hematopoietic necrosis virus (IHNV)
The main objective of this study was to assess correlates of innate resistance in rainbow trout full-sibling families that differ in susceptibility to Infectious hematopoietic necrosis virus (IHNV). As part of a commercial breeding program, full-sibling families were challenged with IHNV by waterborne exposure at the 1 g size to determine susceptibility to IHNV. Progeny from select families (N = 7
Authors
M. K. Purcell, S. E. LaPatra, J.C. Woodson, Gael Kurath, J. R. Winton
Feasibility of Surgically Implanting Acoustic Tags into Pacific Herring
Internally implanted acoustic tags represent a potentially valuable approach to assessing the seasonal migration and distribution patterns of Pacific herring Clupea palasii. We examined the feasibility of implanting two sizes of dummy acoustic tags (9 mm in diameter × 21 mm long, 1.6 g; and 7 mm in diameter × 18 mm long, 0.7 g) in Pacific herring that had been held in captivity for nearly a year a
Authors
Paul K. Hershberger, Jacob L. Gregg, A.C. Seitz, Brenda L. Norcross, J.C. Payne, A.N. Kagley, B Meloy
Release of infectious cells from epidermal ulcers in Ichthyophonus sp.–infected Pacific Herring (Clupea pallasii): Evidence for multiple mechanisms of transmission
A common clinical sign of ichthyophoniasis in herring and trout is “sandpaper” skin, a roughening of the epidermis characterized by the appearance of small papules, followed by ulceration and sloughing of the epithelium; early investigators hypothesized that these ulcers might be a means of transmitting the parasite, Ichthyophonus sp., without the necessity of ingesting an infected host. We examin
Authors
Paul K. Hershberger, Jacob L. Gregg, R. M. Kocan
Differential growth of U and M type infectious haematopoietic necrosis virus in a rainbow trout–derived cell line, RTG-2
Infectious haematopoietic necrosis virus (IHNV) is one of the most important viral pathogens of salmonids. In rainbow trout, IHNV isolates in the M genogroup are highly pathogenic, while U genogroup isolates are significantly less pathogenic. We show here that, at a multiplicity of infection (MOI) of 1, a representative U type strain yielded 42‐fold less infectious virus than an M type strain in t
Authors
Jeong Woo Park, Chang Hoon Moon, Andrew Wargo, Maureen K. Purcell, Gael Kurath
Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy
FTIR and Raman spectromicroscopy were used to characterize the composition of Curvularia protuberata hyphae, and to compare a strain isolated from plants inhabiting geothermal soils with a non-geothermal isolate. Thermal IR source images of hyphae have been acquired with a 64 × 64 element focal plane array detector; single point IR spectra have been obtained with synchrotron source light. In some
Authors
Merrill Isenor, Susan G.W. Kaminsky, Russell J. Rodriguez, Regina S. Redman, Kathleen M. Gough
Chronic and persistent viral hemorrhagic septicemia virus infections in Pacific herring
Chronic viral hemorrhagic septicemia virus (VHSV) infections were established in a laboratory stock of Pacific herring Clupea pallasii held in a large-volume tank supplied with pathogen-free seawater at temperatures ranging from 6.8 to 11.6°C. The infections were characterized by viral persistence for extended periods and near-background levels of host mortality. Infectious virus was recovered fro
Authors
Paul K. Hershberger, Jacob L. Gregg, James R. Winton, Cortney A. Grady, L. Taylor
Kinetics of viral shedding provide insights into the epidemiology of viral hemorrhagic septicemia in Pacific herring
Losses from infectious diseases are an important component of natural mortality among marine fish species, but factors controlling the ecology of these diseases and their potential responses to anthropogenic changes are poorly understood. We used viral hemorrhagic septicemia virus (VHSV) and a laboratory stock of Pacific herring Clupea pallasii to investigate the kinetics of viral shedding and its
Authors
Paul K. Hershberger, Jacob L. Gregg, James R. Winton, Courtney Grady, Rachael Collins
Growth, condition factor, and bioenergetics modeling link warmer stream temperatures below a small dam to reduced performance of juvenile steelhead
We investigated the growth and feeding performance of juvenile steelhead Oncorhynchus mykiss using field measures and bioenergetics modeling. Juvenile steelhead populations were sampled from mid-June through August 2004 at study sites upstream and downstream of Hemlock Dam. The growth and diet of juvenile steelhead were determined for a warm (summer) and subsequent (late summer) transitional perio
Authors
S.T. Sauter, P.J. Connolly
Current lineages of the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells
Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, Sulimanovic, Bearzotti, Muzinic, Zwillenberg, Chilmonczyk, Vautherot & de Kinkelin 1983) has become one of the most widely used tools for research on fish viruses and the diagnosis of fish viral diseases.
Authors
J. Winton, W. Batts, P. DeKinkelin, M. LeBerre, M. Bremont, N. Fijan
Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel)
A 3-primer PCR system was developed to discriminate invasive zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel. The system is based on: 1) universal primers that amplifies a region of the nuclear 28s rDNA gene from both species and 2) a species-specific primer complementary to either zebra or quagga mussel. The species-specific primers bind to sequences between the binding sites
Authors
M.S. Hoy, K. Kelly, R. J. Rodriguez