Publications
Below is a list of available Fish Health Program peer reviewed and published science.
Filter Total Items: 323
Expression kinetics of key genes in the early innate immune response to Great Lakes viral hemorrhagic septicemia virus IVb infection in yellow perch (Perca flavescens)
The recently discovered strain of viral hemorrhagic septicemia virus, VHSV-IVb, represents an example of the introduction of an extremely pathogenic rhabdovirus capable of infecting a wide variety of new fish species in a new host-environment. The goal of the present study was to delineate the expression kinetics of key genes in the innate immune response relative to the very early stages of VHSV-
Authors
Wendy Olson, Eveline Emmenegger, Jolene Glenn, Crystal Simchick, Jim Winton, Frederick Goetz
Comparative susceptibility among three stocks of yellow perch, Perca flavescens (Mitchill), to viral haemorrhagic septicaemia virus strain IVb from the Great Lakes
The Great Lakes strain of viral haemorrhagic septicaemia virus IVb (VHSV-IVb) is capable of infecting a wide number of naive species and has been associated with large fish kills in the Midwestern United States since its discovery in 2005. The yellow perch, Perca flavescens (Mitchill), a freshwater species commonly found throughout inland waters of the United States and prized for its high value i
Authors
W. Olson, E. Emmenegger, J. Glenn, J. Winton, F. Goetz
Bench-top validation testing of selected immunological and molecular Renibacterium salmoninarum diagnostic assays by comparison with quantitative bacteriological culture
No gold standard assay exhibiting error-free classification of results has been identified for detection of Renibacterium salmoninarum, the causative agent of salmonid bacterial kidney disease. Validation of diagnostic assays for R. salmoninarum has been hindered by its unique characteristics and biology, and difficulties in locating suitable populations of reference test animals. Infection status
Authors
D.G. Elliott, L.J. Applegate, A.L. Murray, M. K. Purcell, C.L. McKibben
Emergence of MD type infectious hematopoietic necrosis virus in Washington State coastal steelhead trout
Infectious hematopoietic necrosis virus (IHNV) occurs in North America as 3 major phylogenetic groups designated U, M, and L. In coastal Washington State, IHNV has historically consisted of U genogroup viruses found predominantly in sockeye salmon Oncorhynchus nerka. M genogroup IHNV, which has host-specific virulence for rainbow and steelhead trout O. mykiss, was detected only once in coastal Was
Authors
Rachel Breyta, Amelia Jones, Bruce Stewart, Ray Brunson, Joan Thomas, John Kerwin, Jim Bertolini, Sonia Mumford, Chris Patterson, Gael Kurath
Characterization of the OmyY1 region on the rainbow trout Y chromosome
We characterized the male-specific region on the Y chromosome of rainbow trout, which contains both sdY (the sex-determining gene) and the male-specific genetic marker, OmyY1. Several clones containing the OmyY1 marker were screened from a BAC library from a YY clonal line and found to be part of an 800 kb BAC contig. Using fluorescence in situ hybridization (FISH), these clones were localized to
Authors
Ruth B. Phillips, Jenefer J. DeKoning, Joseph P. Brunelli, Joshua J. Faber-Hammond, John D. Hansen, Kris A. Christensen, Suzy C.P. Renn, Gary H. Thorgaard
Ammocoetes of Pacific lamprey are not susceptible to common fish rhabdoviruses of the U.S. Pacific Northwest
Pacific Lampreys Entosphenus tridentatus have experienced severe population declines in recent years and efforts to develop captive rearing programs are under consideration. However, there is limited knowledge of their life history, ecology, and potential to harbor or transmit pathogens that may cause infectious disease. As a measure of the possible risks associated with introducing wild lampreys
Authors
Gael Kurath, C J. Jolley, Tarin M. Thompson, D. Thompson, A.T. Whitesel, S. Gutenberger, James R. Winton
Analysis of host genetic diversity and viral entry as sources of between-host variation in viral load
Little is known about the factors that drive the high levels of between-host variation in pathogen burden that are frequently observed in viral infections. Here, two factors thought to impact viral load variability, host genetic diversity and stochastic processes linked with viral entry into the host, were examined. This work was conducted with the aquatic vertebrate virus, Infectious hematopoieti
Authors
Andrew R. Wargo, Alison M. Kell, Robert J. Scott, Gary H. Thorgaard, Gael Kurath
Roles of inflammatory caspases during processing of zebrafish interleukin-1β in Francisella noatunensis infection
The interleukin-1 family of cytokines are essential for the control of pathogenic microbes but are also responsible for devastating autoimmune pathologies. Consequently, tight regulation of inflammatory processes is essential for maintaining homeostasis. In mammals, interleukin-1 beta (IL-1β) is primarily regulated at two levels, transcription and processing. The main pathway for processing IL-1β
Authors
Lucia N. Vojtech, Nichole Scharping, James C. Woodson, John D. Hansen
Production and characterization of monoclonal antibodies to IgM of Pacific herring (Clupea pallasii)
Pacific herring (Clupea pallasii) have a central role in the North Pacific ecosystem as a forage fish species and are natural reservoirs of several important finfish pathogens, including Viral hemorrhagic septicemia virus (VHSV). Here, we report the identification of the gene encoding the immunoglobulin mu (IgM) heavy chain, as well as the development and characterization of monoclonal antibodies
Authors
Maureen K. Purcell, Erin S. Bromage, Jessica Silva, John D. Hansen, Samantha M. Badil, James C. Woodson, Paul K. Hershberger
Susceptibility of Pacific herring to viral hemorrhagic septicemia is influenced by diet
Groups of specific-pathogen-free Pacific herring Clupea pallasii were highly susceptible to infection by viral hemorrhagic septicemia virus (VHSV); however, the level of mortality was influenced by diet during the 40–71 d before, during, and after the first exposure to the virus. Cumulative mortality was highest among the herring maintained on an experimental soy-based pellet, intermediate among t
Authors
Joshua Beaulaurier, N. Bickford, J.L. Gregg, C.A. Grady, A.L. Gannam, J. R. Winton, P.K. Hershberger
A novel antibody-based biomarker for chronic algal toxin exposure and sub-acute neurotoxicity
The neurotoxic amino acid, domoic acid (DA), is naturally produced by marine phytoplankton and presents a significant threat to the health of marine mammals, seabirds and humans via transfer of the toxin through the foodweb. In humans, acute exposure causes a neurotoxic illness known as amnesic shellfish poisoning characterized by seizures, memory loss, coma and death. Regular monitoring for high
Authors
Kathi A. Lefebvre, Elizabeth R. Frame, Frances Gulland, John D. Hansen, Preston S. Kendrick, Richard P. Beyer, Theo K. Bammler, Frederico M. Farin, Emma M. Hiolski, Donald R. Smith, David J. Marcinek
Viral tropism and pathology associated with viral hemorrhagic septicemia in larval and juvenile Pacific herring
Viral hemorrhagic septicemia virus (VHSV) genotype IVa causes mass mortality in wild Pacific herring, a species of economic value, in the Northeast Pacific Ocean. Young of the year herring are particularly susceptible and can be carriers of the virus. To understand its pathogenesis, tissue and cellular tropisms of VHSV in larval and juvenile Pacific herring were investigated with immunohistochemis
Authors
Jan Lovy, N.L. Lewis, P.K. Hershberger, W. Bennett, T.R. Meyers, K.A. Garver