Publications
Filter Total Items: 41
Shale gas development has limited effects on stream biology and geochemistry in a gradient-based, multiparameter study in Pennsylvania
The number of horizontally drilled shale oil and gas wells in the United States has increased from nearly 28,000 in 2007 to nearly 127,000 in 2017, and research has suggested the potential for the development of shale resources to affect nearby stream ecosystems. However, the ability to generalize current studies is limited by the small geographic scope as well as limited breadth and integration o
Authors
Adam Mumford, Kelly O. Maloney, Denise M. Akob, Sarah Nettemann, Arianne Proctor, Jason Ditty, Luke Ulsamer, Josh Lookenbill, Isabelle M. Cozzarelli
Binning singletons: Mentoring through networking at ASM microbe 2019
The American Society for Microbiology (ASM) national conference, Microbe, is the flagship meeting for microbiologists across the globe. The presence of roughly 10,000 attendees provides enormous opportunities for networking and learning. However, such a large meeting can be intimidating to many, especially early career scientists, students, those attending alone, and those from historically underr
Authors
Joseph B. James, Amanda L. Gunn, Denise M. Akob
Draft genome sequence of the Mn(II)-oxidizing bacterium Oxalobacteraceae sp. AB_14
Biological Mn(II) oxidation produces reactive manganese oxides that help to mitigate metal contamination in the environment. Here we present the genome of Oxalobacteraceae sp. AB_14, a species of Mn(II)-oxidizing bacteria (MOB) notable for its ability to catalyze Mn oxidation at low pH (5.5).
Authors
Timothy Bushman, Denise M. Akob, Tsing Bohu, Andrea Beyer, Tanja Woyke, Nicole Shapiro, Alla Lapidus, Hans-Peter Klenk, Kirsten Küsel
Phylogenetic techniques in geomicrobiology
Molecular biological techniques have revolutionized the field of geomicrobiology by providing researchers with robust techniques for identifying microorganisms and characterizing microbial communities in a wide variety of environments. These techniques have freed researchers from the constraints of classical culture-based microbiology and allowed the discovery of previously unknown phylogenetic di
Authors
Denise M. Akob, Adam C. Mumford, Darren S. Dunlop, Amisha T. Poret-Peterson
Organic geochemistry and toxicology of a stream impacted by unconventional oil and gas wastewater disposal operations
Water and sediment extracts samples were analyzed for extractable hydrocarbons by gas chromatography/mass spectrometry (GC/MS) using an Agilent (Agilent Technologies, Palo Alto, CA, USA) 7890 series GC and 5975 electron ionization (EI) mass selective detector (MSD) operated in scan mode. Agilent ChemStation software was used for data acquisition and analysis (version E.02.00.493 on GC/MS computer
Authors
William H. Orem, Matthew S. Varonka, Lynn M. Crosby, Karl B. Haase, Keith A. Loftin, Michelle L. Hladik, Denise M. Akob, Calin Tatu, Adam C. Mumford, Jeanne B. Jaeschke, Anne L. Bates, Tiffani Schell, Isabelle M. Cozzarelli
Microbial community composition of a hydrocarbon reservoir 40 years after a CO2 enhanced oil recovery flood
Injecting CO2 into depleted oil reservoirs to extract additional crude oil is a common enhanced oil recovery (CO2-EOR) technique. However, little is known about how in situ microbial communities may be impacted by CO2 flooding, or if any permanent microbiological changes occur after flooding has ceased. Formation water was collected from an oil field that was flooded for CO2-EOR in the 1980s, incl
Authors
Jenna L. Shelton, Robert S. Andrews, Denise M. Akob, Christina A. DeVera, Adam C. Mumford, John E. McCray, Jennifer C. McIntosh
An initial comparison of pesticides and amphibian pathogens between natural and created wetlands in the New Jersey Pinelands, 2014–16
A study conducted by the U.S. Geological Survey, in cooperation with the New Jersey Pinelands Commission and Montclair State University, was designed to compare pesticide concentrations and the presence and prevalence of amphibian pathogens between natural ponds and two types of created wetlands, excavated ponds and stormwater basins, throughout the New Jersey Pinelands. The study described herein
Authors
Kelly L. Smalling, John F. Bunnell, Jonathan Cohl, Kristin M. Romanok, Lisa Hazard, Kirsten Monsen, Denise M. Akob, Angela M. Hansen, Michelle L. Hladik, Nicole Abdallah, Quratulain Ahmed, Araba Assan, Matthew D. De Parsia, Amaryl Griggs, Megan McWayne-Holmes, Naisargi Patel, Corey Sanders, Yesha Shrestha, Sean M. Stout, Brianna Williams
Acetylenotrophy: A hidden but ubiquitous microbial metabolism?
Acetylene (IUPAC name: ethyne) is a colorless, gaseous hydrocarbon, composed of two triple bonded carbon atoms attached to hydrogens (C2H2). When microbiologists and biogeochemists think of acetylene, they immediately think of its use as an inhibitory compound of certain microbial processes and a tracer for nitrogen fixation. However, what is less widely known is that anaerobic and aerobic microor
Authors
Denise M. Akob, John M. Sutton, Janna L. Fierst, Karl B. Haase, Shaun Baesman, George Luther, Laurence G. Miller, Ronald S. Oremland
Common hydraulic fracturing fluid additives alter the structure and function of anaerobic microbial communities
The development of unconventional oil and gas (UOG) resources results in the production of large volumes of wastewater containing a complex mixture of hydraulic fracturing chemical additives and components from the formation. The release of these wastewaters into the environment poses potential risks that are poorly understood. Microbial communities in stream sediments form the base of the food ch
Authors
Adam C. Mumford, Denise M. Akob, J. Grace Klinges, Isabelle M. Cozzarelli
Degradation of crude 4-MCHM (4-methylcyclohexanemethanol) in sediments from Elk River, West Virginia
In January 2014, approximately 37 800 L of crude 4-methylcyclohexanemethanol (crude MCHM) spilled into the Elk River, West Virginia. To understand the long-term fate of 4-MCHM, we conducted experiments under environmentally relevant conditions to assess the potential for the 2 primary compounds in crude MCHM (1) to undergo biodegradation and (2) for sediments to serve as a long-term source of 4-MC
Authors
Isabelle M. Cozzarelli, Denise M. Akob, Mary Jo Baedecker, Tracey Spencer, Jeanne B. Jaeschke, Darren S. Dunlap, Adam C. Mumford, Amisha T. Poret-Peterson, Douglas B. Chambers
Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93
Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting a mixture of terrestrial and marine emissions from anthropogenic, biomass-burning, and unidentified biogenic sources. Fermentation of acetylene was serendipitously discovered during C2H2 block assays of N2O reductase, and Pelobacter acetylenicus was shown to grow on C2H2 via acetylene hydratase (AH). AH
Authors
Denise M. Akob, Shaun Baesman, John M. Sutton, Janna L. Fierst, Adam Mumford, Yesha Shrestha, Amisha T. Poret-Peterson, Stacy C. Bennett, Darren S. Dunlap, Karl B. Haase, Ronald S. Oremland
Archaeal diversity and CO2 fixers in carbonate-/siliciclastic-rock groundwater ecosystems
Groundwater environments provide habitats for diverse microbial communities, and although Archaea usually represent a minor fraction of communities, they are involved in key biogeochemical cycles. We analysed the archaeal diversity within a mixed carbonate-rock/siliciclastic-rock aquifer system, vertically from surface soils to subsurface groundwater including aquifer and aquitard rocks. Archaeal
Authors
Cassandre Sara Lazar, Wenke Stoll, Robert Lehmann, Martina Herrmann, Valérie F. Schwab, Denise M. Akob, Ali Nawaz, Tesfaye Wubet, François Buscot, Kai-Uwe Totsche, Kirsten Küsel