Deborah D Iwanowicz, PhD
Dr. Deborah D. Iwanowicz is a research fish biologist that utilizes molecular diagnostics for research studies.
Her research principally focuses on targeted amplicon sequencing applied to topics such as: diet evaluation, pollen identification, pathogen identification and microbial community profiling. She is a principle investigator and collaborator on a number of projects that focus on the effects of contaminants on fish health, the significance of disease organisms in invasive species, profiling complex microbial community dynamics, as well as protocol and assay development. Her research includes that of molecular microbiology (parasitology, bacteriology, virology on fishes, amphibians, sea turtles, marine mammals, insects, plants, aquatic and terrestrial gastropods). She leads efforts in the NFHRL for method development on metagenomics analyses of diet from scat, stomach content, or regurgitated cough pellets. This work has been modified to identify plant species found in pollen, diets of mammals, birds, and aquatic animals. She also often focuses her efforts on threatened and endangered species.
Education and Certifications
Ph.D., 2007, University of Georgia
M.S., 1998, Purdue University
B.S., 1993, Purdue University
Science and Products
Potential concerns with analytical Methods Used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA
A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding
Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, United States
Complete genome sequence of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola
Metabarcoding of fecal samples to determine herbivore diets: A case study of the endangered Pacific pocket mouse
Effects of urban land-use on largescale stonerollers in the Mobile River Basin, Birmingham, AL
Taxonomic characterization of honey bee (Apis mellifera) pollen foraging based on non-overlapping paired-end sequencing of nuclear ribosomal loci
Identifying plant taxa that honey bees (Apis mellifera) forage upon is of great apicultural interest, but traditional methods are labor intensive and may lack resolution. Here we evaluate a high-throughput genetic barcoding approach to characterize trap-collected pollen from multiple North Dakota apiaries across multiple years. We used the Illumina MiSeq platform to generate sequence scaffolds fro
Transforming growth factor-β1 expression in endangered age-0 shortnose suckers (Chasmistes brevirostris) from Upper Klamath Lake, OR relative to histopathology, meristic, spatial, and temporal data
Evidence of estrogenic endocrine disruption in smallmouth and largemouth bass inhabiting Northeast U.S. National Wildlife Refuge waters: A reconnaissance study
Mycobacterial infection in Northern snakehead (Channa argus) from the Potomac River catchment
Spread of the Rat Lungworm (Angiostrongylus cantonensis) in Giant African Land Snails (Lissachatina fulica) in Florida, USA
Efficacy of iodine for disinfection of Lake Sturgeon eggs from the St. Lawrence River, New York
Non-USGS Publications**
https://www.lsc.usgs.gov/files/D%20Iwanowicz%202011.pdf
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Potential concerns with analytical Methods Used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA
A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding
Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, United States
Complete genome sequence of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola
Metabarcoding of fecal samples to determine herbivore diets: A case study of the endangered Pacific pocket mouse
Effects of urban land-use on largescale stonerollers in the Mobile River Basin, Birmingham, AL
Taxonomic characterization of honey bee (Apis mellifera) pollen foraging based on non-overlapping paired-end sequencing of nuclear ribosomal loci
Identifying plant taxa that honey bees (Apis mellifera) forage upon is of great apicultural interest, but traditional methods are labor intensive and may lack resolution. Here we evaluate a high-throughput genetic barcoding approach to characterize trap-collected pollen from multiple North Dakota apiaries across multiple years. We used the Illumina MiSeq platform to generate sequence scaffolds fro
Transforming growth factor-β1 expression in endangered age-0 shortnose suckers (Chasmistes brevirostris) from Upper Klamath Lake, OR relative to histopathology, meristic, spatial, and temporal data
Evidence of estrogenic endocrine disruption in smallmouth and largemouth bass inhabiting Northeast U.S. National Wildlife Refuge waters: A reconnaissance study
Mycobacterial infection in Northern snakehead (Channa argus) from the Potomac River catchment
Spread of the Rat Lungworm (Angiostrongylus cantonensis) in Giant African Land Snails (Lissachatina fulica) in Florida, USA
Efficacy of iodine for disinfection of Lake Sturgeon eggs from the St. Lawrence River, New York
Non-USGS Publications**
https://www.lsc.usgs.gov/files/D%20Iwanowicz%202011.pdf
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.