Justin Greer is a Computational Biologist at the Western Fisheries Research Center. He conducts research on the effects of environmental stressors on the health of aquatic populations. Current research focuses on the effects of stormwater pollutants on salmonid health and strategies to reduce toxic stormwater inputs.
Justin B. Greer, Ph.D.
Justin Greer is a Computational Biologist at the Western Fisheries Research Center. He conducts research on the effects of environmental stressors on the health of aquatic populations. Current research focuses on the effects of stormwater pollutants on salmonid health and strategies to reduce toxic stormwater inputs.
Dr. Greer uses various laboratory approaches, including in vivo and in vitro studies, to address the effects of environmental pollutants on fish health. Contaminants contained within stormwater that are derived from car tires, such as 6PPD-quinone, have been shown to be highly toxic to some salmon species. His laboratory is focused on investigating the mechanisms by which these contaminants exert their toxicity to better understand and mitigate their environmental impacts. They leverage biochemical and molecular techniques in their investigations. High-throughput molecular sequencing techniques are frequently used to investigate sublethal effects and generate further testable hypotheses. Dr. Greer’s research also emphasizes the utility of alternatives to animal models, such as in vitro cell culture, to improve our biological understanding while reducing the use of animals in research.
Currently, a primary focus of the lab is identification of suitable alternative chemicals for car tires with reduced impacts on salmon and the environment. The lab is committed to working with State, Federal, and Industry partners on these efforts to improve the health of aquatic environments. Dr. Greer also utilizes informatics and large data analyses to investigate other novel questions related to environmental health, including host-pathogen interactions and impacts of thermal stress.
Professional Experience
2019 – Present: Computational Biologist, U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA
2018 – 2019: Postdoctoral Scholar, University of California Riverside, Riverside, CA
Education and Certifications
Ph.D. Marine Biology and Ecology, University of Miami, Miami, FL
B.S. Biology and Chemistry, University of Miami, Miam, FL
Science and Products
Structurally selective ozonolysis of p-phenylenediamines and toxicity in coho salmon and rainbow trout
Toxicity of crude oil-derived polar unresolved complex mixtures to Pacific herring embryos: Insights beyond polycyclic aromatic hydrocarbons
Tire-derived contaminants 6PPD and 6PPD-Q: Analysis, sample handling, and reconnaissance of United States stream exposures
Structurally selective ozonolysis of p-phenylenediamines and toxicity in coho salmon and rainbow trout
Effects of temperature on viral load, inclusion body formation, and host response in Pacific Herring with viral erythrocytic necrosis (VEN)
Tire-derived transformation product 6PPD-quinone induces mortality and transcriptionally disrupts vascular permeability pathways in developing coho salmon
Establishing an in vitro model to assess the toxicity of 6PPD-quinone and other tire wear transformation products
Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection
Brief oil exposure reduces fitness in wild Gulf of Mexico mahi-mahi (Coryphaena hippurus)
Expression plasticity regulates intraspecific variation in the acclimatization potential of a reef-building coral
The developing zebrafish kidney is impaired by Deepwater Horizon crude oil early-life stage exposure: A molecular to whole-organism perspective
Exposure to Deepwater Horizon crude oil increases free cholesterol in larval red drum (Sciaenops ocellatus)
USGS Research on 6PPD-quinone: Where the Rubber Meets the Road
Preliminary Research Sheds Light on Proper Analysis and Sample Handling for the Tire-Derived Contaminants 6PPD and 6PPD-quinone
Evaluation of Lethal and Sublethal Effects of 6PPDQ on Coastal Cutthroat Trout (Oncorhynchus clarkii clarkii) (ver. 2.0, March 2025)
Concentrations of 6PPD and 6PPD-Quinone in a United States reconnaissance of stormwater, surface water, and groundwater, 2018-24
Mortality, morphology, and water chemistry for 6PPD-quinone exposed coho embryos
Assessing cell line models for species differences in 6PPD-quinone sensitivity
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.
Justin Greer is a Computational Biologist at the Western Fisheries Research Center. He conducts research on the effects of environmental stressors on the health of aquatic populations. Current research focuses on the effects of stormwater pollutants on salmonid health and strategies to reduce toxic stormwater inputs.

Rainfall mobilizes roadway contaminants that flow from storm drains into aquatic habitats. Photo taken in the Pacific Northeast
Rainfall mobilizes roadway contaminants that flow from storm drains into aquatic habitats. Photo taken in the Pacific Northeast

Juvenile Coho salmon (Oncorhynchus kisutch) behavior is assessed following exposure to 6PPD-quinone, a derivative of the compound 6PPD which is used in tires to prevent wear.
Juvenile Coho salmon (Oncorhynchus kisutch) behavior is assessed following exposure to 6PPD-quinone, a derivative of the compound 6PPD which is used in tires to prevent wear.
Juvenile Coho salmon (Oncorhynchus kisutch) exhibiting abnormal behaviors associated with exposure to 6PPD-quinone, a derivative of the compound 6PPD which is used in tires to prevent wear.
Juvenile Coho salmon (Oncorhynchus kisutch) exhibiting abnormal behaviors associated with exposure to 6PPD-quinone, a derivative of the compound 6PPD which is used in tires to prevent wear.
Science and Products
Structurally selective ozonolysis of p-phenylenediamines and toxicity in coho salmon and rainbow trout
Toxicity of crude oil-derived polar unresolved complex mixtures to Pacific herring embryos: Insights beyond polycyclic aromatic hydrocarbons
Tire-derived contaminants 6PPD and 6PPD-Q: Analysis, sample handling, and reconnaissance of United States stream exposures
Structurally selective ozonolysis of p-phenylenediamines and toxicity in coho salmon and rainbow trout
Effects of temperature on viral load, inclusion body formation, and host response in Pacific Herring with viral erythrocytic necrosis (VEN)
Tire-derived transformation product 6PPD-quinone induces mortality and transcriptionally disrupts vascular permeability pathways in developing coho salmon
Establishing an in vitro model to assess the toxicity of 6PPD-quinone and other tire wear transformation products
Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection
Brief oil exposure reduces fitness in wild Gulf of Mexico mahi-mahi (Coryphaena hippurus)
Expression plasticity regulates intraspecific variation in the acclimatization potential of a reef-building coral
The developing zebrafish kidney is impaired by Deepwater Horizon crude oil early-life stage exposure: A molecular to whole-organism perspective
Exposure to Deepwater Horizon crude oil increases free cholesterol in larval red drum (Sciaenops ocellatus)
USGS Research on 6PPD-quinone: Where the Rubber Meets the Road
Preliminary Research Sheds Light on Proper Analysis and Sample Handling for the Tire-Derived Contaminants 6PPD and 6PPD-quinone
Evaluation of Lethal and Sublethal Effects of 6PPDQ on Coastal Cutthroat Trout (Oncorhynchus clarkii clarkii) (ver. 2.0, March 2025)
Concentrations of 6PPD and 6PPD-Quinone in a United States reconnaissance of stormwater, surface water, and groundwater, 2018-24
Mortality, morphology, and water chemistry for 6PPD-quinone exposed coho embryos
Assessing cell line models for species differences in 6PPD-quinone sensitivity
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.
Justin Greer is a Computational Biologist at the Western Fisheries Research Center. He conducts research on the effects of environmental stressors on the health of aquatic populations. Current research focuses on the effects of stormwater pollutants on salmonid health and strategies to reduce toxic stormwater inputs.
Justin Greer is a Computational Biologist at the Western Fisheries Research Center. He conducts research on the effects of environmental stressors on the health of aquatic populations. Current research focuses on the effects of stormwater pollutants on salmonid health and strategies to reduce toxic stormwater inputs.

Rainfall mobilizes roadway contaminants that flow from storm drains into aquatic habitats. Photo taken in the Pacific Northeast
Rainfall mobilizes roadway contaminants that flow from storm drains into aquatic habitats. Photo taken in the Pacific Northeast

Juvenile Coho salmon (Oncorhynchus kisutch) behavior is assessed following exposure to 6PPD-quinone, a derivative of the compound 6PPD which is used in tires to prevent wear.
Juvenile Coho salmon (Oncorhynchus kisutch) behavior is assessed following exposure to 6PPD-quinone, a derivative of the compound 6PPD which is used in tires to prevent wear.
Juvenile Coho salmon (Oncorhynchus kisutch) exhibiting abnormal behaviors associated with exposure to 6PPD-quinone, a derivative of the compound 6PPD which is used in tires to prevent wear.
Juvenile Coho salmon (Oncorhynchus kisutch) exhibiting abnormal behaviors associated with exposure to 6PPD-quinone, a derivative of the compound 6PPD which is used in tires to prevent wear.