Luke R Iwanowicz, Ph.D. (Former Employee)
Science and Products
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Methods used for the collection and analysis of chemical and biological data for the Tapwater Exposure Study, United States, 2016–17
In 2016, the U.S. Geological Survey (USGS) Environmental Health Mission Area, initiated the Tapwater Exposure Study as part of an infrastructure project to assess human exposure to potential threats from complex mixtures of contaminants. In the pilot phase (2016), samples were collected from 11 States throughout the United States, and in the second phase (2017), the study focused on the Greater Ch
Authors
Kristin M. Romanok, Dana W. Kolpin, Shannon M. Meppelink, Maria Argos, Juliane B. Brown, Michael J. Devito, Julie E. Dietze, Carrie E. Givens, James L. Gray, Christopher P. Higgins, Michelle L. Hladik, Luke R. Iwanowicz, Keith A. Loftin, R. Blaine McCleskey, Carrie A. McDonough, Michael T. Meyer, Mark J. Strynar, Christopher P. Weis, Vickie S. Wilson, Paul M. Bradley
Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola
The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cypri
Authors
Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Teresa Lewis, Tom Brandt, Lakyn R. Sanders, Robert S. Cornman
Indicators of exposure to estrogenic compounds at Great Lakes Areas of Concern: Species and site comparisons
Adverse effects resulting from potential exposure of wild fishes to estrogenic endocrine disruptors was assessed at seven United States Great Lakes Areas of Concern using biomarkers ranging from organismal (gonadosomatic indices) to tissue/plasma (histology, plasma vitellogenin) and molecular (hepatic gene transcripts) levels. Biomonitoring was conducted on pelagic, top predator species, largemout
Authors
Vicki S. Blazer, Heather L. Walsh, Cassidy H. Shaw, Luke R. Iwanowicz, Ryan P. Braham, Patricia Mazik
A method for determining avian influenza virus hemagglutinin and neuraminidase subtype association
Methods for grouping specific avian influenza virus (AIV) hemagglutinin (HA) and neuraminidase (NA) subtype reverse-transcription polymerase chain reaction (RT-PCR) products into HA:NA subtypes when egg incubation is technically not feasible were evaluated. These approaches were adopted for use as post hoc methods after melt curve analysis. The methods are based on ratios obtained from amplicon co
Authors
Christopher Ottinger, Deborah D. Iwanowicz, Luke R. Iwanowicz, Cynthia R. Adams, Lakyn R. Sanders, Christine L. Densmore
Temporal evaluation of estrogenic endocrine disruption markers in smallmouth bass (Micropterus dolomieu) reveals seasonal variability in intersex
A reconnaissance project completed in 2009 identified intersex and elevated plasma vitellogenin in male smallmouth bass inhabiting the Missisquoi River, VT. In an attempt to identify the presence and seasonality of putative endocrine disrupting chemicals or other factors associated with these observations, a comprehensive reevaluation was conducted between September 2012 and June 2014. Here, we co
Authors
Luke R. Iwanowicz, A.E. Pinkney, C.P. Guy, A.M. Major, K. Munney, Vicki S. Blazer, David Alvarez, Heather L. Walsh, Adam J. Sperry, Lakyn R. Sanders, D. R. Smith
An evaluation of the toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fish and their forage base
Potash, with the active ingredient potassium chloride (KCl) is a chemical that is currently being evaluated for potential use as a molluscicide to combat invasive zebra mussels and quagga mussels in Western United States waters. Although data available for other freshwater fishes indicate that recommended treatment levels of potash as a molluscicide are sublethal, this has not been demonstrated fo
Authors
Christine L. Densmore, Luke R. Iwanowicz, Anne P. Henderson, Vicki S. Blazer, Baileigh M. Reed-Grimmett, Lakyn R. Sanders
Pharmaceuticals, hormones, pesticides, and other bioactive contaminants in water, sediment, and tissue from Rocky Mountain National Park, 2012–2013
Pharmaceuticals, hormones, pesticides, and other bioactive contaminants (BCs) are commonly detected in surface water and bed sediment in urban and suburban areas, but these contaminants are understudied in remote locations. In Rocky Mountain National Park (RMNP), Colorado, USA, BCs may threaten the reproductive success and survival of native aquatic species, benthic communities, and pelagic food w
Authors
William A. Battaglin, Paul M. Bradley, Luke R. Iwanowicz, Celeste A. Journey, Heather L. Walsh, Vicki S. Blazer
Exposure to synthetic hydraulic fracturing waste influences the mucosal bacterial community structure of the brook trout (Salvelinus fontinalis) epidermis
Production of natural gas using unconventional technologies has risen as demand for alternative fuels has increased. Impacts on the environment from waste generated from these processes are largely unexplored. In particular, the outcomes of organismal exposure to hydraulic fracturing waste have not been rigorously evaluated. We evaluated the effects of exposure to surrogate hydraulic fracturing wa
Authors
Heather S. Galbraith, Deborah D. Iwanowicz, Daniel E. Spooner, Luke R. Iwanowicz, David Keller, Paula M. Zelanko, Cynthia R. Adams
Genomic evolution, recombination, and inter-strain diversity of chelonid alphaherpesvirus 5 from Florida and Hawaii green sea turtles with fibropapillomatosis
Chelonid alphaherpesvirus 5 (ChHV5) is a herpesvirus associated with fibropapillomatosis (FP) in sea turtles worldwide. Single-locus typing has previously shown differentiation between Atlantic and Pacific strains of this virus, with low variation within each geographic clade. However, a lack of multi-locus genomic sequence data hinders understanding of the rate and mechanisms of ChHV5 evolutionar
Authors
Cheryl L. Morrison, Luke R. Iwanowicz, Thierry M. Work, Elizabeth Fahsbender, Mya Breitbart, Cynthia R. Adams, Deborah D. Iwanowicz, Lakyn Sanders, Mathias Ackermann, Robert S. Cornman
Contaminant-associated health effects in fishes from the Ottawa and Ashtabula Rivers, Ohio
The health of resident fishes serves as a biologically relevant barometer of aquatic ecosystem integrity. Here, the health of the Ottawa River and Ashtabula River (both within the Lake Erie Basin) were assessed using morphological and immunological biomarkers in brown bullheads (Ameiurus nebulosus) and largemouth bass (Micropterus salmoides). Biomarker metrics were compared to fish collected from
Authors
Luke R. Iwanowicz, Vicki S. Blazer, Heather L. Walsh, Cassidy H. Shaw, David S. DeVault, Jo A. Banda
Investigating the influence of nitrate nitrogen on post-smolt Atlantic salmon Salmo salar reproductive physiology in water recirculation aquaculture systems
A major issue affecting land-based, closed containment Atlantic salmon Salmo salar growout production in water recirculation aquaculture systems (RAS) is precocious male maturation, which can negatively impact factors such as feed conversion, fillet yield, and product quality. Along with other water quality parameters, elevated nitrate nitrogen (NO3-N) has been shown to influence the reproductive
Authors
Christopher Good, John Davidson, Luke R. Iwanowicz, Michael T. Meyer, Julie E. Dietze, Dana W. Kolpin, David Marancik, Jill Birkett, Christina Williams, Steven T. Summerfelt
Draft genome sequence of a picorna-like virus associated with gill tissue in clinically normal brook trout, Salvelinus fontinalis
Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.
Authors
Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Heather S. Galbraith, Aaron Aunins, Robert S. Cornman
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 13
Filter Total Items: 22
No Result Found
Filter Total Items: 103
Methods used for the collection and analysis of chemical and biological data for the Tapwater Exposure Study, United States, 2016–17
In 2016, the U.S. Geological Survey (USGS) Environmental Health Mission Area, initiated the Tapwater Exposure Study as part of an infrastructure project to assess human exposure to potential threats from complex mixtures of contaminants. In the pilot phase (2016), samples were collected from 11 States throughout the United States, and in the second phase (2017), the study focused on the Greater Ch
Authors
Kristin M. Romanok, Dana W. Kolpin, Shannon M. Meppelink, Maria Argos, Juliane B. Brown, Michael J. Devito, Julie E. Dietze, Carrie E. Givens, James L. Gray, Christopher P. Higgins, Michelle L. Hladik, Luke R. Iwanowicz, Keith A. Loftin, R. Blaine McCleskey, Carrie A. McDonough, Michael T. Meyer, Mark J. Strynar, Christopher P. Weis, Vickie S. Wilson, Paul M. Bradley
Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola
The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cypri
Authors
Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Teresa Lewis, Tom Brandt, Lakyn R. Sanders, Robert S. Cornman
Indicators of exposure to estrogenic compounds at Great Lakes Areas of Concern: Species and site comparisons
Adverse effects resulting from potential exposure of wild fishes to estrogenic endocrine disruptors was assessed at seven United States Great Lakes Areas of Concern using biomarkers ranging from organismal (gonadosomatic indices) to tissue/plasma (histology, plasma vitellogenin) and molecular (hepatic gene transcripts) levels. Biomonitoring was conducted on pelagic, top predator species, largemout
Authors
Vicki S. Blazer, Heather L. Walsh, Cassidy H. Shaw, Luke R. Iwanowicz, Ryan P. Braham, Patricia Mazik
A method for determining avian influenza virus hemagglutinin and neuraminidase subtype association
Methods for grouping specific avian influenza virus (AIV) hemagglutinin (HA) and neuraminidase (NA) subtype reverse-transcription polymerase chain reaction (RT-PCR) products into HA:NA subtypes when egg incubation is technically not feasible were evaluated. These approaches were adopted for use as post hoc methods after melt curve analysis. The methods are based on ratios obtained from amplicon co
Authors
Christopher Ottinger, Deborah D. Iwanowicz, Luke R. Iwanowicz, Cynthia R. Adams, Lakyn R. Sanders, Christine L. Densmore
Temporal evaluation of estrogenic endocrine disruption markers in smallmouth bass (Micropterus dolomieu) reveals seasonal variability in intersex
A reconnaissance project completed in 2009 identified intersex and elevated plasma vitellogenin in male smallmouth bass inhabiting the Missisquoi River, VT. In an attempt to identify the presence and seasonality of putative endocrine disrupting chemicals or other factors associated with these observations, a comprehensive reevaluation was conducted between September 2012 and June 2014. Here, we co
Authors
Luke R. Iwanowicz, A.E. Pinkney, C.P. Guy, A.M. Major, K. Munney, Vicki S. Blazer, David Alvarez, Heather L. Walsh, Adam J. Sperry, Lakyn R. Sanders, D. R. Smith
An evaluation of the toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fish and their forage base
Potash, with the active ingredient potassium chloride (KCl) is a chemical that is currently being evaluated for potential use as a molluscicide to combat invasive zebra mussels and quagga mussels in Western United States waters. Although data available for other freshwater fishes indicate that recommended treatment levels of potash as a molluscicide are sublethal, this has not been demonstrated fo
Authors
Christine L. Densmore, Luke R. Iwanowicz, Anne P. Henderson, Vicki S. Blazer, Baileigh M. Reed-Grimmett, Lakyn R. Sanders
Pharmaceuticals, hormones, pesticides, and other bioactive contaminants in water, sediment, and tissue from Rocky Mountain National Park, 2012–2013
Pharmaceuticals, hormones, pesticides, and other bioactive contaminants (BCs) are commonly detected in surface water and bed sediment in urban and suburban areas, but these contaminants are understudied in remote locations. In Rocky Mountain National Park (RMNP), Colorado, USA, BCs may threaten the reproductive success and survival of native aquatic species, benthic communities, and pelagic food w
Authors
William A. Battaglin, Paul M. Bradley, Luke R. Iwanowicz, Celeste A. Journey, Heather L. Walsh, Vicki S. Blazer
Exposure to synthetic hydraulic fracturing waste influences the mucosal bacterial community structure of the brook trout (Salvelinus fontinalis) epidermis
Production of natural gas using unconventional technologies has risen as demand for alternative fuels has increased. Impacts on the environment from waste generated from these processes are largely unexplored. In particular, the outcomes of organismal exposure to hydraulic fracturing waste have not been rigorously evaluated. We evaluated the effects of exposure to surrogate hydraulic fracturing wa
Authors
Heather S. Galbraith, Deborah D. Iwanowicz, Daniel E. Spooner, Luke R. Iwanowicz, David Keller, Paula M. Zelanko, Cynthia R. Adams
Genomic evolution, recombination, and inter-strain diversity of chelonid alphaherpesvirus 5 from Florida and Hawaii green sea turtles with fibropapillomatosis
Chelonid alphaherpesvirus 5 (ChHV5) is a herpesvirus associated with fibropapillomatosis (FP) in sea turtles worldwide. Single-locus typing has previously shown differentiation between Atlantic and Pacific strains of this virus, with low variation within each geographic clade. However, a lack of multi-locus genomic sequence data hinders understanding of the rate and mechanisms of ChHV5 evolutionar
Authors
Cheryl L. Morrison, Luke R. Iwanowicz, Thierry M. Work, Elizabeth Fahsbender, Mya Breitbart, Cynthia R. Adams, Deborah D. Iwanowicz, Lakyn Sanders, Mathias Ackermann, Robert S. Cornman
Contaminant-associated health effects in fishes from the Ottawa and Ashtabula Rivers, Ohio
The health of resident fishes serves as a biologically relevant barometer of aquatic ecosystem integrity. Here, the health of the Ottawa River and Ashtabula River (both within the Lake Erie Basin) were assessed using morphological and immunological biomarkers in brown bullheads (Ameiurus nebulosus) and largemouth bass (Micropterus salmoides). Biomarker metrics were compared to fish collected from
Authors
Luke R. Iwanowicz, Vicki S. Blazer, Heather L. Walsh, Cassidy H. Shaw, David S. DeVault, Jo A. Banda
Investigating the influence of nitrate nitrogen on post-smolt Atlantic salmon Salmo salar reproductive physiology in water recirculation aquaculture systems
A major issue affecting land-based, closed containment Atlantic salmon Salmo salar growout production in water recirculation aquaculture systems (RAS) is precocious male maturation, which can negatively impact factors such as feed conversion, fillet yield, and product quality. Along with other water quality parameters, elevated nitrate nitrogen (NO3-N) has been shown to influence the reproductive
Authors
Christopher Good, John Davidson, Luke R. Iwanowicz, Michael T. Meyer, Julie E. Dietze, Dana W. Kolpin, David Marancik, Jill Birkett, Christina Williams, Steven T. Summerfelt
Draft genome sequence of a picorna-like virus associated with gill tissue in clinically normal brook trout, Salvelinus fontinalis
Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.
Authors
Luke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Heather S. Galbraith, Aaron Aunins, Robert S. Cornman
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government