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Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples
We developed a primer and probe based quantitative PCR assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The assay targets a region in the mitochondrial DNA (mtDNA) D-loop gene. Tests of the assay were performed in silico (using the NCBI BLAST tool), in vitro (using DNA extracted from
Authors
Marshal Hoy, Carl Ostberg
Human and infrastructure exposure to large wildfires in the United States
An increasing number of wildfire disasters have occurred in recent years in the United States. Here we demonstrate that cumulative primary human exposure—the population residing within the perimeters of large wildfires—was 594,850 people from 2000 to 2019 across the contiguous United States (CONUS), 82% of which occurred in the western United States. Primary population exposure increased by 125% i
Authors
Arash Modaresi Rad, John T. Abatzoglou, Jason R. Kreitler, Mohammad Reza Alizadeh, Amir AghaKouchak, Nicholas Hudyma, NIcholas Nauslar, Mojtaba Sadegh
Earthquake scenarios for Quito, Ecuador; Cali, Colombia; and Santiago De Los Caballeros, Dominican Republic
Earthquake risk associated with Quito, Ecuador; Cali, Colombia; and Santiago de los Caballeros, Dominican Republic is examined by generating a set of hypothetical earthquake scenarios considering seismic sources, recent seismicity, and major historical earthquakes recorded in the vicinity. In this study, particular focus is given to the development of earthquake scenarios for use in emergency plan
Authors
Robert Edward Chase, Kishor S. Jaiswal, Alejandro Calderon, Hugo Yepes, Loren Goddard, Catalina Yepes-Estrada
Validation and standardization of SPE and HPLC-UV methods for simultaneous determination of legacy and insensitive munitions
There have been few attempts to consolidate legacy and insensitive munitions analyses. Furthermore, there are no standard methods for insensitive munitions (IM) in tissues, resulting in overlapping methods and supplementary analyses. The goal of the present study was to validate extraction and instrumental methods previously developed and address analytical methodology gaps (missing tissue matrice
Authors
Austin Scircle, Ashley Kimble, Jared C. Smith, Bobbi Stromer, Samuel Beal, Jay Clausen, Thomas Georgian, Adam Mumford, Geoffrey Giarmo, Martin Peterson, Hart Hedgpeth, Rebecca Crouch, Anthony J. Bednar
2023 Coastal master plan: ICM-wetlands – Submerged aquatic vegetation (SAV) updates
Submerged aquatic vegetation (SAV) provides critical structural habitat for valuable nekton and wildlife species across coastal ecosystems and can buffer the negative effects of land loss. Landscape change and restoration efforts across coastal Louisiana can impact the occurrence, coverage, and species assemblages of SAV, and changes to these foundational species can have cascading impacts across
Authors
Kristin DeMarco, Donald Schoolmaster, Brady Couvillion
2023 Coastal master plan: Landscape input data
Coastal Louisiana is a complex landscape. The composition of the landscape, as well as the processes which influence said landscape, vary in both space and time. The models used in the 2023 Coastal Master Plan must attempt to reflect that spatial and temporal variability. It is therefore of the utmost importance that the spatial data sets upon which the models are initialized are of the highest qu
Authors
Brady Couvillion
Vortex trapping of sand grains over ripples under oscillatory flow
Sand ripples significantly impact morphodynamics in the nearshore by generating coherent vortices, which can transport suspended sediment to greater heights in the water column than above flat beds. Coherent vortices can trap sediment grains if the settling velocity of the grain is smaller than the maximum vertical fluid velocity in the vortex (Nielsen 1992). Particle image and tracking velocimetr
Authors
Donya P. Frank-Gilchrist, Allison Penko, Margaret Louise Palmsten, Joseph Calantoni
Automated georeferencing and feature extraction of geologic maps and mineral sites
The predictive power of mineral prospectivity analysis depends on high quality, spatially accurate, analysis-ready datasets. Of paramount importance are geologic maps and mineral site data, but the state of readiness for utilizing these datasets remains sub-optimal for advanced computational techniques. As the U.S. Geological Survey (USGS) fulfils its mission to map the distribution of critical mi
Authors
Graham W. Lederer, Joshua Mark Rosera, Margaret A. Goldman, Garth E. Graham, Asitang Mishra, Amanda Towler, Brian Wilson, Dustin Graf, Michael Milano, Elizabeth Roberts, Gabrielle Hedrick, Carsten Oertel, Anastassios Dardas, Thomas McEntee
The "H," "A," and "B" of a HAB: A definitional framework
The use of the phrase “harmful algal bloom” and the acronym HAB originated in the marine science world, and referred to blooms also known as red tides, which can kill fish and sea life. The organisms that make up marine HABs generally do not thrive in lakes. In freshwater, HABs are most often associated with blooms of toxin-producing cyanobacteria. The term HAB started to be used broadly in the ea
Authors
Rebecca Michelle Gorney, Jennifer L. Graham, Jennifer C. Murphy
2023 PyLith Hackathon report
The 3rd Pylith Hackathon was held June 12–17, 2023, at the Colorado School of Mines in Golden, Colorado with funding from the Computational Infrastructure for Geodynamics (CIG). The hackathon involved 17 participants working on 5 different projects to implement new features and create new examples for the PyLith crustal deformation modeling software. The projects included (1) spontaneous rupture u
Authors
Brad T. Aagaard
Hydrologic framework and characterization of the Little Colorado River alluvial aquifer near Leupp, Arizona
The Little Colorado River alluvial aquifer near Leupp, Arizona, was investigated as a possible source of irrigation water for the Leupp and Birdsprings Chapters of the Navajo Nation. The physical, chemical, and hydraulic characteristics of the alluvial aquifer were studied using geophysical surveys, installation of observation wells, water-level measurements, chemical analyses, groundwater pumping
Authors
Jon P. Mason, Jeffrey R. Kennedy, Jamie P. Macy, Bruce Gungle
Winners and losers over a ½ century of change in crayfish assemblages of Wyoming, USA
Crayfish have experienced extensive assemblage reorganization as a result of global change, with some species becoming globally invasive and others becoming rare or extinct. We combined historical and contemporary sampling data to determine temporal trends of crayfish assemblages of Wyoming, USA, identifying winners and losers over a ½ century of change (1969–2020). We first documented range expan
Authors
Braxton Newkirk, Eric R. Larson, Andrew D. Walker, Annika W. Walters