Infographic depicts multiple ways 6PPD can enter the environment, as well as scientists taking water samples.
Rachael F Lane, Ph.D.
Dr. Rachael Lane is an analytical chemist whose research focuses on the identification, fate, and transport of emerging organic contaminants.
EDUCATION
- Ph.D. 2015, with Honors, Analytical Chemistry, University of Kansas
- B.S. 2009, cum laude, Chemistry, Emporia State University (minor: Geospatial Analysis)
PUBLICATIONS
- Lane, R.F., Adams, C.D., Randtke, S.J., Carter Jr., R.E. 2015 Chlorination and chloramination of bisphenol A, bisphenol F, and bisphenol A diglycidyl ether in drinking water. Water Research, 79: 68-78. https://doi.org/10.1016/j.watres.2015.04.014
- Lane, R.F., Adams, C.D., Randtke, S.J., Carter, Jr., R.E. 2014. Bisphenol diglycidyl ethers and bisphenol A and their hydrolysis in drinking water. Water Research, 75: 331-339. https://doi.org/10.1016/j.watres.2014.09.043
- Roach, J.D., Lane, R.F., Hussain, Y. 2011. Comparative study of the uses of poly(4-vinylpyridine) and poly(diallyldimethylammonium) chloride for the removal of perchlorate from aqueous solution by polyelectrolyte-enhanced ultrafiltration. Water Research, 45(3): 1387-1393. https://doi.org/10.1016/j.watres.2010.10.027
- Rosen, B.H., Loftin, K.A., Smith, C.E., Lane, R.F., Keydel, S.P. 2010. Microphotographs of cyanobacteria documenting the effects of various cell-lysis techniques. U.S. Geological Survey Open-File Report, 2010–1289, 1-203. https://pubs.usgs.gov/of/2010/1289/
Science and Products
Filter Total Items: 13
Preliminary Research Sheds Light on Proper Analysis and Sample Handling for the Tire-Derived Contaminants 6PPD and 6PPD-quinone
Tire and road wear particles have been shown to cause acute effects to sensitive aquatic animals and degrade their habitats. U.S. Geological Survey (USGS) scientists developed methods to accurately identify aquatic compounds, such as 6PPD and 6PPD-quinone, that can cause acute mortality events in coho salmon (Oncorhynchus kisutch).
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Central Midwest Water Science Center, Eastern Ecological Science Center, Kansas Water Science Center, New Jersey Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Washington Water Science Center, Western Fisheries Research Center
FY25 Capabilities and Services
The U.S. Geological Survey (USGS) Kansas Water Science Center Organic Geochemistry Research Laboratory (OGRL) was established in 1987. The OGRL is a multidisciplinary program that contributes knowledge about the distribution, fate, transport, and effects of new and understudied organic compounds that may affect human health and (or) ecosystems. The OGRL consists of two units: Algal and Other...
From Tread to Watershed: How Tire Wear Particle Chemicals 6PPD and 6PPD-quinone Are Impacting Waterways
Tire and road wear particles (TRWPs) are generated during normal driving conditions and contain both rubber and roadway materials. These particles travel from the roadways and urban environments into surrounding ecosystems where there are deleterious impacts from both the TRWPs and leached rubber chemicals. Recent studies have demonstrated the widespread occurrence and toxicity of TRWPs and their...
Organic Geochemistry Research Core Technology Team
About the Research The Organic Geochemistry Research Laboratory Core Technology Team (CTT) as part of the Environmental Health Program works to develop targeted and non-targeted analytical methods for the identification and quantitation of chemicals that can impact the health of humans and other organisms, and uses bioassays to screen for receptor inhibition.
6PPD-quinone
6PPD-quinone is a compound used to make tires more durable and is also linked to toxicity for Coho Salmon and other aquatic species.
Gestodene Affects Fish Reproductive Behavior in Laboratory Exposure Study
Short-term laboratory exposure of adult fathead minnows to the human contraceptive progestin, gestodene (GES), at environmentally relevant concentrations induced rapid and negative effects on reproductive health and suggests that wild fish may be similarly affected.
OGRL Algal Toxins Methods of Analysis
Algal toxins are a group of toxic compounds produced by a range of photosynthetic freshwater and marine plankton. These toxins have the ability to cause sickness in animals and humans and in severe cases lead to death. OGRL has the ability to evaluate and conduct occurrence, fate, transport, effects, and treatability studies methods.
Glyphosate, AMPA, and Glufosinate Method of Analysis: OGRL LCGY
Glyphosate and glufosinate are herbicides used for weed and vegetation control. AMPA, aminomethylphosphonic acid, is a degradation product of glyphosate. Since the introduction of glyphosate resistant (round-up ready) corn and soybeans glyphosate has become the most widely used herbicide in the world.
Antibiotic Methods of Analysis: OGRL LCAB
Antibiotics are used for human health and livestock management and health. The increase of antibiotic resistant infections has resulted in concern that sub-therapeutic use of antibiotics for weight gain in livestock and the release of low levels of antibiotics into surface and groundwater from urban and agricultural sources may be contributing to this problem.
Parents and Degradation Products of Analysis: OGRL LCEA
Triazines and phenylureas are commonly used herbicides for controlling weeds in row crops. These herbicides and their degradation products have been found in samples collected from surface water, groundwater, and precipitation throughout the United States. Atrazine until recently was the most widely applied herbicide in the U.S. and is still a widely applied preplanting corn herbicide.
Acetamide Parents and Degradation Products of Analysis: OGRL LCPD
Acetamide herbicides are used to control weeds in row crops such as corn and soybeans. Acetamide herbicides and their degradates are common contaminants in surface and groundwater. Some of the widely detected acid degradates are on the USEPA contaminant candidate list (CCL).
OGRL Enzyme Linked-Imunnosorbent Assay Methods of Analysis
Immunoassays are rapid screening techinques that can be used to provide data on a specific compound or class of compunds on a large number of samples. We have used these methods to conduct large scale reconnaissance studies of atrazine in rainfall and cyanobacterial toxins in the USEPA national lake and reservoir reconnaissance study.
Evaluation of Lethal and Sublethal Effects of 6PPD-Q on Coastal Cutthroat Trout (Oncorhynchus clarkii clarkii)
6PPD-Quinone (6PPD-Q) was recently implicated in inducing mass pre-spawn mortality events in adult coho salmon (Oncorhynchus kisutch) in the Pacific Northwest. Since then, studies have reported highly variable toxicity among fish species, including within the salmonids. This dataset explores the sensitivity of coastal cutthroat trout (Oncorhynchus clarkii clarkii) at three different life stages: a
Concentrations of 6PPD and 6PPD-Quinone in a United States reconnaissance of stormwater, surface water, and groundwater, 2018-24
From 2018 to 2024 surface waters (fresh and brackish), groundwater, and stormwater (runoff, re-use) samples were collected from sites with varying landuse (agricultural, forested, and urban) and streamflow conditions and analyzed at the Organic Geochemistry Research Laboratory in Lawrence, Kansas for the tire antioxidant 6PPD (6-p-phenylenediamine; N-(1,3-Dimethylbutyl)-N’-phenyl-p-phenylenediamin
State-Wide Reconnaissance of Per- and Polyfluoroalkyl Substances, N-(1,3-Dimethylbutyl)-N-phenyl-p-phenylenediamine (6PPD), and 6PPD-Quinone in Iowa Streams, 2022-2023
Water-quality samples were collected at 49 surface water sites covering a range of land uses across the state of Iowa starting the summer of 2022 and continuing into the spring of 2023 by staff from the U.S. Geological Survey Central Midwest Water Science Center. Samples were submitted to the National Water Quality Laboratory in Denver, Colorado, and were analyzed for 32 per- and polyfluoroalkyl s
Antibiotic and Antibiotic Resistance Signatures in Iowa Streams, 2019
Chemical and microbiological results, quality assurance and quality control, site location, and method information for surface water, bed sediment, and wastewater effluent collected from 34 stream locations across Iowa (United States). Environmental samples were analyzed for a suite of 29 antibiotics, plated on selective media for 15 types of bacteria growth, and DNA was extracted from culture gro
High-Resolution Mass Spectrometry Data for Wastewater Samples Collected at an on-Site Separator and Storage Tank at the Marcellus Shale Energy and Environment Laboratory (MSEEL) 2015-2019, Morgantown Industrial Park (MIP), West Virginia
The Marcellus Shale Energy and Environment Laboratory (MSEEL) is part of the Northeast Natural Energy LLC (NNE) production facility on the Monongahela River in Monongalia County, West Virginia, USA. Natural gas extraction in the area creates large volumes of wastewater that may contain chemical compounds that pose risks to humans, animals, and the environment. The U. S. Geological Survey (USGS) ha
Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021
In the spring of 2021, six households with private wells were selected throughout Graton, California, based on proximity to agriculture (within one mile of agriculture), well depth (i.e., less than 150 feet in depth), and well type (i.e., dug versus drilled). Silicone bands were deployed for 30 days at outdoor locations at each household to passively sample pesticides in ambient air. On May 3, 202
Assessing cell line models for species differences in 6PPD-quinone sensitivity
Coho salmon have been shown to be highly sensitive to the tire transformation chemical 6PPD-quinone, with concentrations contained in stormwater sufficient to induce mortality in up to 90% of the entire fall coho run. Other salmonid species (chinook, sockeye, chum) are much less sensitive to 6PPD-quinone induced mortality. This data examines the varying species sensitivities across salmoids using
Organic and inorganic constituents in surface water and native and non-native fish and frog health data collected from streams, impoundments and wetlands in the New Jersey Pinelands, 2017-2020 (ver. 1.1, August 2022)
The data presented include concentrations of 122 organic, 23 inorganic constituents, and estrogenicity in water collected from 13 off-stream non-point source sites (natural and created wetlands) and eight on-stream point source sites (streams and impoundments) in the New Jersey Pinelands, 2017-2020. Native and non-native fish health data collected from the on-stream sites as well as native frog da
Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019
Oklahoma State University South-Central Research Station (SCRS) was used to conduct research to understand the chemical composition of various water types and their potential environmental and human health effects. The study area provided the opportunity to study five water types: (1) receiving surface water (Washita River), (2) urban stormwater, (3) wastewater treatment plant effluent used for ir
Water-quality data for a pharmaceutical study at Muddy Creek in North Liberty and Coralville, Iowa, 2017-2018
Samples were collected, processed, and analyzed for organics, estrogen equivalents, and fecal indicator bacteria. Filtered organic samples were sent to the National Water Quality Laboratory in Denver, Colorado. Unfiltered estrogen equivalent samples were sent to the Organic Geochemistry Research Lab in Lawrence, Kansas, for extraction, after which they were sent to the National Fish Health Resear
Infographic depicts multiple ways 6PPD can enter the environment, as well as scientists taking water samples.
Filter Total Items: 14
Tire-derived contaminants 6PPD and 6PPD-Q: Analysis, sample handling, and reconnaissance of United States stream exposures
The environmental ubiquity of tire and road wear particles (TRWP) underscores the need to understand the occurrence, persistence, and environmental effects of tire-related chemicals in aquatic ecosystems. One such chemical is 6PPD-quinone (6PPD-Q), a transformation product of the tire antioxidant 6PPD. In urban stormwater runoff 6PPD-Q can exceed acute toxicity thresholds for several salmonid spec
Authors
Rachael F. Lane, Kelly Smalling, Paul M. Bradley, Justin Blaine Greer, Stephanie Gordon, John Hansen, Dana W. Kolpin, Andrew R. Spanjer, Jason R. Masoner
By
Water Resources Mission Area, Ecosystems Mission Area, Central Midwest Water Science Center, Kansas Water Science Center, New Jersey Water Science Center, Oklahoma-Texas Water Science Center, Washington Water Science Center, Western Fisheries Research Center, South Atlantic Water Science Center (SAWSC)
Integrated science for the study of microplastics in the environment—A strategic science vision for the U.S. Geological Survey
Executive SummaryEvidence of the widespread occurrence of microplastics throughout our environment and exposure to humans and other organisms over the past decade has led to questions about the possibility of health hazards and mitigation of exposures. This document discusses nanoplastics as well as microplastics (referred to solely as microplastics); the microplastics have a range from 1 micromet
Authors
Deborah D. Iwanowicz, Austin K. Baldwin, Larry B. Barber, Vicki S. Blazer, Steven R. Corsi, Joseph W. Duris, Shawn C. Fisher, Michael Focazio, Sarah E. Janssen, Jeramy Roland Jasmann, Dana W. Kolpin, Johanna M. Kraus, Rachael F. Lane, Mari E. Lee, Kristen B. McSwain, Timothy D. Oden, Timothy J. Reilly, Andrew R. Spanjer
Simultaneous stream assessment of antibiotics, bacteria, antibiotic resistant bacteria, and antibiotic resistant genes in an agricultural region of the United States
Antimicrobial resistance is now recognized as a leading global threat to human health. Nevertheless, there currently is a limited understanding of the environment's role in the spread of AMR and antibiotic resistant genes (ARG). In 2019, the U.S. Geological Survey conducted the first statewide assessment of antibiotic resistant bacteria (ARB) and ARGs in surface water and bed sediment collected fr
Authors
Carrie E Givens, Dana W. Kolpin, Laura E. Hubbard, Shannon M. Meppelink, David M. Cwiertny, Darrin A. Thompson, Rachael F. Lane, Michaelah C. Wilson
Tire-derived transformation product 6PPD-quinone induces mortality and transcriptionally disrupts vascular permeability pathways in developing coho salmon
Urban stormwater runoff frequently contains the car tire transformation product 6PPD-quinone, which is highly toxic to juvenile and adult coho salmon (Onchorychus kisutch). However, it is currently unclear if embryonic stages are impacted. We addressed this by exposing developing coho salmon embryos starting at the eyed stage to three concentrations of 6PPD-quinone twice weekly until hatch. Impact
Authors
Justin Blaine Greer, Ellie Maureen Dalsky, Rachael F. Lane, John Hansen
Establishing an in vitro model to assess the toxicity of 6PPD-quinone and other tire wear transformation products
The tire wear transformation product 6PPD-quinone (6PPDQ) has been implicated as the causative factor for broad scale mortality events for coho salmon in the Pacific Northwest. Highly variable sensitivity to 6PPDQ in closely related salmonids complicates efforts to evaluate the broader toxicological impacts to aquatic ecosystems. Our goals were to (1) validate the large range of in vivo species se
Authors
Justin Blaine Greer, Ellie Maureen Dalsky, Rachael F. Lane, John Hansen
Juxtaposition of intensive agriculture, vulnerable aquifers, and mixed chemical/microbial exposures in private-well tapwater in northeast Iowa
In the United States and globally, contaminant exposure in unregulated private-well point-of-use tapwater (TW) is a recognized public-health data gap and an obstacle to both risk-management and homeowner decision making. To help address the lack of data on broad contaminant exposures in private-well TW from hydrologically-vulnerable (alluvial, karst) aquifers in agriculturally-intensive landscapes
Authors
Paul M. Bradley, Dana W. Kolpin, Darrin A. Thompson, Kristin M. Romanok, Kelly L. Smalling, Sara E. Breitmeyer, Mary C. Cardon, David M. Cwiertny, Nicola Evans, R. William Field, Michael J. Focazio, Laura E. Beane Freeman, Carrie E Givens, James L. Gray, Gordon L. Hager, Michelle Hladik, Jonathan N. Hoffman, Rena R. Jones, Leslie K. Kanagy, Rachael F. Lane, R. Blaine McCleskey, Danielle Medgyesi, Elizabeth Medlock-Kakaley, Shannon M. Meppelink, Michael T. Meyer, Diana A. Stavreva, Mary H. Ward
Contaminant exposure and transport from three potential reuse waters within a single watershed
Global demand for safe and sustainable water supplies necessitates a better understanding of contaminant exposures in potential reuse waters. In this study, we compared exposures and load contributions to surface water from the discharge of three reuse waters (wastewater effluent, urban stormwater, and agricultural runoff). Results document substantial and varying organic-chemical contribution to
Authors
Jason R. Masoner, Dana W. Kolpin, Isabelle M. Cozzarelli, Paul M. Bradley, Brian Arnall, Kenneth J. Forshay, James L. Gray, Justin F. Groves, Michelle Hladik, Laura E. Hubbard, Luke R. Iwanowicz, Jeanne B. Jaeschke, Rachael F. Lane, R. Blaine McCleskey, Bridgette F. Polite, David A. Roth, Michael Pettijohn, Michaelah C. Wilson
By
Water Resources Mission Area, Environmental Health Program, Eastern Ecological Science Center, Colorado Water Science Center, California Water Science Center, Central Midwest Water Science Center, Geology, Energy & Minerals Science Center, Kansas Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, Reston Biogeochemical Processes in Groundwater Laboratory
Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream
Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic and terrestrial organisms but also continually expose them to complex mixtures of pharmaceuticals that can potentially impair growth, behavior, and reproduction. Currently, few biomarkers are available that relate to pharmaceutical-specific mechanisms of action. In the experiment reported in this pape
Authors
Emma B. Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory H. LeFevre, Dana W. Kolpin, Hui Zhi, Shannon M. Meppelink, Rachael F. Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca D. Klaper
RNA-seq reveals potential gene biomarkers in fathead minnows (Pimephales promelas) for exposure to treated wastewater effluent
Discharged wastewater treatment plant (WWTP) effluent greatly contributes to the generation of complex mixtures of contaminants of emerging concern (CECs) in aquatic environments which often contain neuropharmaceuticals and other emerging contaminants that may impact neurological function. However, there is a paucity of knowledge on the neurological impacts of these exposures to aquatic organisms.
Authors
Peter Schumann, E. Meade, H. Zhi, G. H. LeFevre, Dana W. Kolpin, Shannon M. Meppelink, Luke R. Iwanowicz, Rachael F. Lane, A. Schmoldt, O. Mueller, R. D. Klaper
Detecting algal toxins and organic contaminants of concern in the environment
The U.S. Geological Survey (USGS) Kansas Water Science Center Organic Geochemistry Research Laboratory (OGRL) was established in 1987. The OGRL is a multidisciplinary program that contributes knowledge about the distribution, fate, transport, and effects of new and understudied organic compounds that may affect human health and (or) ecosystems. The OGRL consists of two units: Algal and Other Envir
Authors
Julie E. Dietze, Rachael F. Lane, Keith A. Loftin, Daniel L. Tush, Michaelah C. Wilson
Food, beverage, and feedstock processing facility wastewater: A unique and underappreciated source of contaminants to U.S. streams
Process wastewaters from food, beverage, and feedstock facilities, although regulated, are an under-investigated environmental contaminant source. Food process wastewaters (FPWWs) from 23 facilities in 17 U.S. states were sampled and documented for a plethora of chemical and microbial contaminants. Of the 576 analyzed organics, 184 (32%) were detected at least once, with concentrations as large as
Authors
Laura E. Hubbard, Dana W. Kolpin, Carrie E Givens, Bradley D. Blackwell, Paul M. Bradley, James L. Gray, Rachael F. Lane, Jason R. Masoner, R. Blaine McCleskey, Kristin M. Romanok, Mark W. Sandstrom, Kelly L. Smalling, Daniel L. Villeneuve
By
Ecosystems Mission Area, Water Resources Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Central Midwest Water Science Center, Kansas Water Science Center, New Jersey Water Science Center, Pennsylvania Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, National Water Quality Laboratory
Pilot-scale expanded assessment of inorganic and organic tapwater exposures and predicted effects in Puerto Rico, USA
A pilot-scale expanded target assessment of mixtures of inorganic and organic contaminants in point-of-consumption drinking water (tapwater, TW) was conducted in Puerto Rico (PR) to continue to inform TW exposures and corresponding estimations of cumulative human-health risks across the US. In August 2018, a spatial synoptic pilot assessment of than 524 organic, 37 inorganic, and select microbiolo
Authors
Paul M. Bradley, Ingrid Y. Padilla, Kristin M. Romanok, Kelly Smalling, Michael J. Focazio, Sara E. Breitmeyer, Mary C. Cardon, Justin M. Conley, Nicola Evans, Carrie E Givens, James L. Gray, L. Earl Gray, Phillip C. Hartig, Michelle Hladik, Christopher P. Higgins, Luke R. Iwanowicz, Rachael F. Lane, Keith Loftin, R. Blaine McCleskey, Carrie A. McDonough, Elizabeth Medlock-Kakaley, Shannon M. Meppelink, Christopher P. Weis, Vickie S. Wilson
Science and Products
Filter Total Items: 13
Preliminary Research Sheds Light on Proper Analysis and Sample Handling for the Tire-Derived Contaminants 6PPD and 6PPD-quinone
Tire and road wear particles have been shown to cause acute effects to sensitive aquatic animals and degrade their habitats. U.S. Geological Survey (USGS) scientists developed methods to accurately identify aquatic compounds, such as 6PPD and 6PPD-quinone, that can cause acute mortality events in coho salmon (Oncorhynchus kisutch).
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Central Midwest Water Science Center, Eastern Ecological Science Center, Kansas Water Science Center, New Jersey Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Washington Water Science Center, Western Fisheries Research Center
FY25 Capabilities and Services
The U.S. Geological Survey (USGS) Kansas Water Science Center Organic Geochemistry Research Laboratory (OGRL) was established in 1987. The OGRL is a multidisciplinary program that contributes knowledge about the distribution, fate, transport, and effects of new and understudied organic compounds that may affect human health and (or) ecosystems. The OGRL consists of two units: Algal and Other...
From Tread to Watershed: How Tire Wear Particle Chemicals 6PPD and 6PPD-quinone Are Impacting Waterways
Tire and road wear particles (TRWPs) are generated during normal driving conditions and contain both rubber and roadway materials. These particles travel from the roadways and urban environments into surrounding ecosystems where there are deleterious impacts from both the TRWPs and leached rubber chemicals. Recent studies have demonstrated the widespread occurrence and toxicity of TRWPs and their...
Organic Geochemistry Research Core Technology Team
About the Research The Organic Geochemistry Research Laboratory Core Technology Team (CTT) as part of the Environmental Health Program works to develop targeted and non-targeted analytical methods for the identification and quantitation of chemicals that can impact the health of humans and other organisms, and uses bioassays to screen for receptor inhibition.
6PPD-quinone
6PPD-quinone is a compound used to make tires more durable and is also linked to toxicity for Coho Salmon and other aquatic species.
Gestodene Affects Fish Reproductive Behavior in Laboratory Exposure Study
Short-term laboratory exposure of adult fathead minnows to the human contraceptive progestin, gestodene (GES), at environmentally relevant concentrations induced rapid and negative effects on reproductive health and suggests that wild fish may be similarly affected.
OGRL Algal Toxins Methods of Analysis
Algal toxins are a group of toxic compounds produced by a range of photosynthetic freshwater and marine plankton. These toxins have the ability to cause sickness in animals and humans and in severe cases lead to death. OGRL has the ability to evaluate and conduct occurrence, fate, transport, effects, and treatability studies methods.
Glyphosate, AMPA, and Glufosinate Method of Analysis: OGRL LCGY
Glyphosate and glufosinate are herbicides used for weed and vegetation control. AMPA, aminomethylphosphonic acid, is a degradation product of glyphosate. Since the introduction of glyphosate resistant (round-up ready) corn and soybeans glyphosate has become the most widely used herbicide in the world.
Antibiotic Methods of Analysis: OGRL LCAB
Antibiotics are used for human health and livestock management and health. The increase of antibiotic resistant infections has resulted in concern that sub-therapeutic use of antibiotics for weight gain in livestock and the release of low levels of antibiotics into surface and groundwater from urban and agricultural sources may be contributing to this problem.
Parents and Degradation Products of Analysis: OGRL LCEA
Triazines and phenylureas are commonly used herbicides for controlling weeds in row crops. These herbicides and their degradation products have been found in samples collected from surface water, groundwater, and precipitation throughout the United States. Atrazine until recently was the most widely applied herbicide in the U.S. and is still a widely applied preplanting corn herbicide.
Acetamide Parents and Degradation Products of Analysis: OGRL LCPD
Acetamide herbicides are used to control weeds in row crops such as corn and soybeans. Acetamide herbicides and their degradates are common contaminants in surface and groundwater. Some of the widely detected acid degradates are on the USEPA contaminant candidate list (CCL).
OGRL Enzyme Linked-Imunnosorbent Assay Methods of Analysis
Immunoassays are rapid screening techinques that can be used to provide data on a specific compound or class of compunds on a large number of samples. We have used these methods to conduct large scale reconnaissance studies of atrazine in rainfall and cyanobacterial toxins in the USEPA national lake and reservoir reconnaissance study.
Evaluation of Lethal and Sublethal Effects of 6PPD-Q on Coastal Cutthroat Trout (Oncorhynchus clarkii clarkii)
6PPD-Quinone (6PPD-Q) was recently implicated in inducing mass pre-spawn mortality events in adult coho salmon (Oncorhynchus kisutch) in the Pacific Northwest. Since then, studies have reported highly variable toxicity among fish species, including within the salmonids. This dataset explores the sensitivity of coastal cutthroat trout (Oncorhynchus clarkii clarkii) at three different life stages: a
Concentrations of 6PPD and 6PPD-Quinone in a United States reconnaissance of stormwater, surface water, and groundwater, 2018-24
From 2018 to 2024 surface waters (fresh and brackish), groundwater, and stormwater (runoff, re-use) samples were collected from sites with varying landuse (agricultural, forested, and urban) and streamflow conditions and analyzed at the Organic Geochemistry Research Laboratory in Lawrence, Kansas for the tire antioxidant 6PPD (6-p-phenylenediamine; N-(1,3-Dimethylbutyl)-N’-phenyl-p-phenylenediamin
State-Wide Reconnaissance of Per- and Polyfluoroalkyl Substances, N-(1,3-Dimethylbutyl)-N-phenyl-p-phenylenediamine (6PPD), and 6PPD-Quinone in Iowa Streams, 2022-2023
Water-quality samples were collected at 49 surface water sites covering a range of land uses across the state of Iowa starting the summer of 2022 and continuing into the spring of 2023 by staff from the U.S. Geological Survey Central Midwest Water Science Center. Samples were submitted to the National Water Quality Laboratory in Denver, Colorado, and were analyzed for 32 per- and polyfluoroalkyl s
Antibiotic and Antibiotic Resistance Signatures in Iowa Streams, 2019
Chemical and microbiological results, quality assurance and quality control, site location, and method information for surface water, bed sediment, and wastewater effluent collected from 34 stream locations across Iowa (United States). Environmental samples were analyzed for a suite of 29 antibiotics, plated on selective media for 15 types of bacteria growth, and DNA was extracted from culture gro
High-Resolution Mass Spectrometry Data for Wastewater Samples Collected at an on-Site Separator and Storage Tank at the Marcellus Shale Energy and Environment Laboratory (MSEEL) 2015-2019, Morgantown Industrial Park (MIP), West Virginia
The Marcellus Shale Energy and Environment Laboratory (MSEEL) is part of the Northeast Natural Energy LLC (NNE) production facility on the Monongahela River in Monongalia County, West Virginia, USA. Natural gas extraction in the area creates large volumes of wastewater that may contain chemical compounds that pose risks to humans, animals, and the environment. The U. S. Geological Survey (USGS) ha
Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021
In the spring of 2021, six households with private wells were selected throughout Graton, California, based on proximity to agriculture (within one mile of agriculture), well depth (i.e., less than 150 feet in depth), and well type (i.e., dug versus drilled). Silicone bands were deployed for 30 days at outdoor locations at each household to passively sample pesticides in ambient air. On May 3, 202
Assessing cell line models for species differences in 6PPD-quinone sensitivity
Coho salmon have been shown to be highly sensitive to the tire transformation chemical 6PPD-quinone, with concentrations contained in stormwater sufficient to induce mortality in up to 90% of the entire fall coho run. Other salmonid species (chinook, sockeye, chum) are much less sensitive to 6PPD-quinone induced mortality. This data examines the varying species sensitivities across salmoids using
Organic and inorganic constituents in surface water and native and non-native fish and frog health data collected from streams, impoundments and wetlands in the New Jersey Pinelands, 2017-2020 (ver. 1.1, August 2022)
The data presented include concentrations of 122 organic, 23 inorganic constituents, and estrogenicity in water collected from 13 off-stream non-point source sites (natural and created wetlands) and eight on-stream point source sites (streams and impoundments) in the New Jersey Pinelands, 2017-2020. Native and non-native fish health data collected from the on-stream sites as well as native frog da
Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019
Oklahoma State University South-Central Research Station (SCRS) was used to conduct research to understand the chemical composition of various water types and their potential environmental and human health effects. The study area provided the opportunity to study five water types: (1) receiving surface water (Washita River), (2) urban stormwater, (3) wastewater treatment plant effluent used for ir
Water-quality data for a pharmaceutical study at Muddy Creek in North Liberty and Coralville, Iowa, 2017-2018
Samples were collected, processed, and analyzed for organics, estrogen equivalents, and fecal indicator bacteria. Filtered organic samples were sent to the National Water Quality Laboratory in Denver, Colorado. Unfiltered estrogen equivalent samples were sent to the Organic Geochemistry Research Lab in Lawrence, Kansas, for extraction, after which they were sent to the National Fish Health Resear
6PPD-quinone infographic
Infographic depicts multiple ways 6PPD can enter the environment, as well as scientists taking water samples.
Infographic depicts multiple ways 6PPD can enter the environment, as well as scientists taking water samples.
Filter Total Items: 14
Tire-derived contaminants 6PPD and 6PPD-Q: Analysis, sample handling, and reconnaissance of United States stream exposures
The environmental ubiquity of tire and road wear particles (TRWP) underscores the need to understand the occurrence, persistence, and environmental effects of tire-related chemicals in aquatic ecosystems. One such chemical is 6PPD-quinone (6PPD-Q), a transformation product of the tire antioxidant 6PPD. In urban stormwater runoff 6PPD-Q can exceed acute toxicity thresholds for several salmonid spec
Authors
Rachael F. Lane, Kelly Smalling, Paul M. Bradley, Justin Blaine Greer, Stephanie Gordon, John Hansen, Dana W. Kolpin, Andrew R. Spanjer, Jason R. Masoner
By
Water Resources Mission Area, Ecosystems Mission Area, Central Midwest Water Science Center, Kansas Water Science Center, New Jersey Water Science Center, Oklahoma-Texas Water Science Center, Washington Water Science Center, Western Fisheries Research Center, South Atlantic Water Science Center (SAWSC)
Integrated science for the study of microplastics in the environment—A strategic science vision for the U.S. Geological Survey
Executive SummaryEvidence of the widespread occurrence of microplastics throughout our environment and exposure to humans and other organisms over the past decade has led to questions about the possibility of health hazards and mitigation of exposures. This document discusses nanoplastics as well as microplastics (referred to solely as microplastics); the microplastics have a range from 1 micromet
Authors
Deborah D. Iwanowicz, Austin K. Baldwin, Larry B. Barber, Vicki S. Blazer, Steven R. Corsi, Joseph W. Duris, Shawn C. Fisher, Michael Focazio, Sarah E. Janssen, Jeramy Roland Jasmann, Dana W. Kolpin, Johanna M. Kraus, Rachael F. Lane, Mari E. Lee, Kristen B. McSwain, Timothy D. Oden, Timothy J. Reilly, Andrew R. Spanjer
Simultaneous stream assessment of antibiotics, bacteria, antibiotic resistant bacteria, and antibiotic resistant genes in an agricultural region of the United States
Antimicrobial resistance is now recognized as a leading global threat to human health. Nevertheless, there currently is a limited understanding of the environment's role in the spread of AMR and antibiotic resistant genes (ARG). In 2019, the U.S. Geological Survey conducted the first statewide assessment of antibiotic resistant bacteria (ARB) and ARGs in surface water and bed sediment collected fr
Authors
Carrie E Givens, Dana W. Kolpin, Laura E. Hubbard, Shannon M. Meppelink, David M. Cwiertny, Darrin A. Thompson, Rachael F. Lane, Michaelah C. Wilson
Tire-derived transformation product 6PPD-quinone induces mortality and transcriptionally disrupts vascular permeability pathways in developing coho salmon
Urban stormwater runoff frequently contains the car tire transformation product 6PPD-quinone, which is highly toxic to juvenile and adult coho salmon (Onchorychus kisutch). However, it is currently unclear if embryonic stages are impacted. We addressed this by exposing developing coho salmon embryos starting at the eyed stage to three concentrations of 6PPD-quinone twice weekly until hatch. Impact
Authors
Justin Blaine Greer, Ellie Maureen Dalsky, Rachael F. Lane, John Hansen
Establishing an in vitro model to assess the toxicity of 6PPD-quinone and other tire wear transformation products
The tire wear transformation product 6PPD-quinone (6PPDQ) has been implicated as the causative factor for broad scale mortality events for coho salmon in the Pacific Northwest. Highly variable sensitivity to 6PPDQ in closely related salmonids complicates efforts to evaluate the broader toxicological impacts to aquatic ecosystems. Our goals were to (1) validate the large range of in vivo species se
Authors
Justin Blaine Greer, Ellie Maureen Dalsky, Rachael F. Lane, John Hansen
Juxtaposition of intensive agriculture, vulnerable aquifers, and mixed chemical/microbial exposures in private-well tapwater in northeast Iowa
In the United States and globally, contaminant exposure in unregulated private-well point-of-use tapwater (TW) is a recognized public-health data gap and an obstacle to both risk-management and homeowner decision making. To help address the lack of data on broad contaminant exposures in private-well TW from hydrologically-vulnerable (alluvial, karst) aquifers in agriculturally-intensive landscapes
Authors
Paul M. Bradley, Dana W. Kolpin, Darrin A. Thompson, Kristin M. Romanok, Kelly L. Smalling, Sara E. Breitmeyer, Mary C. Cardon, David M. Cwiertny, Nicola Evans, R. William Field, Michael J. Focazio, Laura E. Beane Freeman, Carrie E Givens, James L. Gray, Gordon L. Hager, Michelle Hladik, Jonathan N. Hoffman, Rena R. Jones, Leslie K. Kanagy, Rachael F. Lane, R. Blaine McCleskey, Danielle Medgyesi, Elizabeth Medlock-Kakaley, Shannon M. Meppelink, Michael T. Meyer, Diana A. Stavreva, Mary H. Ward
Contaminant exposure and transport from three potential reuse waters within a single watershed
Global demand for safe and sustainable water supplies necessitates a better understanding of contaminant exposures in potential reuse waters. In this study, we compared exposures and load contributions to surface water from the discharge of three reuse waters (wastewater effluent, urban stormwater, and agricultural runoff). Results document substantial and varying organic-chemical contribution to
Authors
Jason R. Masoner, Dana W. Kolpin, Isabelle M. Cozzarelli, Paul M. Bradley, Brian Arnall, Kenneth J. Forshay, James L. Gray, Justin F. Groves, Michelle Hladik, Laura E. Hubbard, Luke R. Iwanowicz, Jeanne B. Jaeschke, Rachael F. Lane, R. Blaine McCleskey, Bridgette F. Polite, David A. Roth, Michael Pettijohn, Michaelah C. Wilson
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Water Resources Mission Area, Environmental Health Program, Eastern Ecological Science Center, Colorado Water Science Center, California Water Science Center, Central Midwest Water Science Center, Geology, Energy & Minerals Science Center, Kansas Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, Reston Biogeochemical Processes in Groundwater Laboratory
Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream
Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic and terrestrial organisms but also continually expose them to complex mixtures of pharmaceuticals that can potentially impair growth, behavior, and reproduction. Currently, few biomarkers are available that relate to pharmaceutical-specific mechanisms of action. In the experiment reported in this pape
Authors
Emma B. Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory H. LeFevre, Dana W. Kolpin, Hui Zhi, Shannon M. Meppelink, Rachael F. Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca D. Klaper
RNA-seq reveals potential gene biomarkers in fathead minnows (Pimephales promelas) for exposure to treated wastewater effluent
Discharged wastewater treatment plant (WWTP) effluent greatly contributes to the generation of complex mixtures of contaminants of emerging concern (CECs) in aquatic environments which often contain neuropharmaceuticals and other emerging contaminants that may impact neurological function. However, there is a paucity of knowledge on the neurological impacts of these exposures to aquatic organisms.
Authors
Peter Schumann, E. Meade, H. Zhi, G. H. LeFevre, Dana W. Kolpin, Shannon M. Meppelink, Luke R. Iwanowicz, Rachael F. Lane, A. Schmoldt, O. Mueller, R. D. Klaper
Detecting algal toxins and organic contaminants of concern in the environment
The U.S. Geological Survey (USGS) Kansas Water Science Center Organic Geochemistry Research Laboratory (OGRL) was established in 1987. The OGRL is a multidisciplinary program that contributes knowledge about the distribution, fate, transport, and effects of new and understudied organic compounds that may affect human health and (or) ecosystems. The OGRL consists of two units: Algal and Other Envir
Authors
Julie E. Dietze, Rachael F. Lane, Keith A. Loftin, Daniel L. Tush, Michaelah C. Wilson
Food, beverage, and feedstock processing facility wastewater: A unique and underappreciated source of contaminants to U.S. streams
Process wastewaters from food, beverage, and feedstock facilities, although regulated, are an under-investigated environmental contaminant source. Food process wastewaters (FPWWs) from 23 facilities in 17 U.S. states were sampled and documented for a plethora of chemical and microbial contaminants. Of the 576 analyzed organics, 184 (32%) were detected at least once, with concentrations as large as
Authors
Laura E. Hubbard, Dana W. Kolpin, Carrie E Givens, Bradley D. Blackwell, Paul M. Bradley, James L. Gray, Rachael F. Lane, Jason R. Masoner, R. Blaine McCleskey, Kristin M. Romanok, Mark W. Sandstrom, Kelly L. Smalling, Daniel L. Villeneuve
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Ecosystems Mission Area, Water Resources Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Central Midwest Water Science Center, Kansas Water Science Center, New Jersey Water Science Center, Pennsylvania Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, National Water Quality Laboratory
Pilot-scale expanded assessment of inorganic and organic tapwater exposures and predicted effects in Puerto Rico, USA
A pilot-scale expanded target assessment of mixtures of inorganic and organic contaminants in point-of-consumption drinking water (tapwater, TW) was conducted in Puerto Rico (PR) to continue to inform TW exposures and corresponding estimations of cumulative human-health risks across the US. In August 2018, a spatial synoptic pilot assessment of than 524 organic, 37 inorganic, and select microbiolo
Authors
Paul M. Bradley, Ingrid Y. Padilla, Kristin M. Romanok, Kelly Smalling, Michael J. Focazio, Sara E. Breitmeyer, Mary C. Cardon, Justin M. Conley, Nicola Evans, Carrie E Givens, James L. Gray, L. Earl Gray, Phillip C. Hartig, Michelle Hladik, Christopher P. Higgins, Luke R. Iwanowicz, Rachael F. Lane, Keith Loftin, R. Blaine McCleskey, Carrie A. McDonough, Elizabeth Medlock-Kakaley, Shannon M. Meppelink, Christopher P. Weis, Vickie S. Wilson