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These publications showcase the significant science conducted in our Science Centers.

Filter Total Items: 16785

Heteropolaria colisarum Foissner and Schubert, 1977 (Protozoa: Epistylididae) or North American freshwater fishes

The peritrichous ciliate ‘Epistylis sp.’ involved in the‘red-sore’ disease of various North American freshwater fishes is identified as Heteropolaria colisarum Foissner & Schubert, 1977. Its morphology is redescribed from observations of living and silver-stained organisms and biometrically analysed individuals. Extended zooids can be easily identified by their highly characteristic (elongated) bo
Authors
W. Foissner, G. L. Hoffman, A.J. Mitchell

Histopathology associated with pyridoxine deficiency in Atlantic salmon (Salmo salar)

Fingerling Atlantic salmon (Salmo solar) fed a production diet deficient in pyridoxine showed several significant effects: increased mortality; behavioral changes; degenerative changes in kidneys, ovaries, and liver; a paucity of thyroid colloid; and hyperplasia of renal hematopoietic tissue. Changes in nerve tissue were equivocal.
Authors
Roger L. Herman

Temperature comparisons for antibody production in vitro by plaque-forming cells from trout Salmo gairdneri (Richardson), and mice

Anterior kidney and splenic cells were taken from rainbow trout and splenic cells from BALB/c mice immunized with a T-dependent (sheep red blood cells) or T-independent (DNP-Ficoll) antigen. The cells were incubated at different temperatures in Jerne plaque assays (direct or passive haemolytic plaque assays). The optimum numbers of in vitro plaque-forming cells (PFC) after incubation with homologo
Authors
F. W. van Ginkel, W. B. van Muiswinkel, B. Merchant, E. F. Lizzio, O. W. Dixon, D. P. Anderson

Rapid serological analysis of bacterial lipopolysaccharides by electrotransfer to nitrocellulose

Techniques are described for the rapid screening of proteinase K-treated bacterial lysates by electroblot and immunoenzymatic detection to assess O-specificity of antigens and antisera. Conditions are outlined which permit the use of a single polyacrylamide gel for both electrotransfer to nitrocellulose and silver staining. Immunodetection of transferred LPS bands was equally sensitive to silver s
Authors
S. W. Pyle, W. B. Schill

Growth, body composition and hepatic tyrosine aminotransferase activity in cortisol-fed channel catfish, Ictalurus punctatus Rafinesque

Metabolic consequences of chronic elevation of cortisol in the diet of yearling channel catfish, Ictalurus punctatus, were studied. Cortisol was incorporated into the diet in concentrations of 1, 10, 50 and 100 μg/g of food. This diet was offered at 3% of the body weight per day for 10 weeks. Fish were individually weighed and measured at 2-week intervals and feeding rates were adjusted. Body weig
Authors
K. B. Davis, P. Torrance, N. C. Parker, M. A. Suttle

Immune response of channel catfish, Ictalurus punctatus Rafinesque, to bacterial and protozoan antigens administered by three routes

Experiments were conducted to measure the agglutinating antibody response of channel catfish to a particulate and a soluble antigen administered simultaneously by one of three routes. Specific antibody production in response to administration of particulate brucella tube-test antigen via intramuscular injection, oral drench, and topical application peaked at 3 weeks in channel catfish held under i
Authors
S. W. Pyle, D. L. Dawe